Method for extracting and purifying fusidic acid

A fusidic acid and extraction technology, which is applied in the field of extracting and purifying fusidic acid, can solve problems such as complex and difficult processes, and achieve the effects of stable process, simple operation, and reduced dosage

Inactive Publication Date: 2019-03-29
宣城菁科生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patents 200680049547.4, 200810045003.1, 201010122886.9 and foreign patents RU1499922C and GB2224649A respectively disclose some production methods for the

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for extracting and purifying fusidic acid, comprising the steps of:

[0046] S1. Prepare dry mycelium: separate the fusidic acid fermentation liquid, take the mycelium, and dry to obtain dry mycelium;

[0047] S2. Preparing a concentrated solution: soaking the dried mycelia obtained in S1 with an organic solvent, and then concentrating the organic solvent to obtain a concentrated solution;

[0048] S3. Preparation of fusidic acid: extracting, decolorizing, concentrating, crystallizing, and drying the concentrated solution obtained in S2 to obtain crude fusidic acid; recrystallizing and drying the crude fusidic acid to obtain fusidic acid.

Embodiment 2

[0050] A method for extracting and purifying fusidic acid, comprising the steps of:

[0051] S1. Preparation of dry mycelium: separate the fusidic acid fermentation broth with pH adjusted to 3, take the mycelium, wash the mycelium with water, and dry the mycelium at a temperature not higher than 60°C to obtain dried bacteria Mycelia, wherein the water content of the dried mycelium is not higher than 15wt%, and the volume ratio of the fusidic acid fermentation broth to water is 1:0.4;

[0052]S2. Preparation of concentrated solution: Soak the dried mycelium obtained in S1 with methanol with a water content not greater than 10wt%, soak for 8 hours, take methanol, adjust the pressure not higher than -0.08MPa, adjust the temperature not higher than 60°C, and depressurize Evaporate to obtain a concentrated solution, wherein the concentration of fusidic acid in the concentrated solution is 40000 μg / ml, and the volume ratio of methanol to fusidic acid fermentation broth is 0.4:1;

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Embodiment 3

[0055] A method for extracting and purifying fusidic acid, comprising the steps of:

[0056] S1. Preparation of dry mycelium: separate the fusidic acid fermentation broth with pH adjusted to 4.5, take the mycelium, wash the mycelium with water, and dry the mycelium at a temperature not higher than 60°C to obtain dried bacteria Mycelium, wherein the water content of the dried mycelium is not higher than 15wt%, and the volume ratio of the fusidic acid fermentation broth to water is 1:0.3;

[0057] S2. Preparation of concentrated solution: Soak the dried mycelium obtained in S1 with ethanol with a water content not greater than 10 wt%, soak for 9 hours, take ethanol, adjust the pressure not higher than -0.08MPa, adjust the temperature not higher than 60°C, and depressurize Evaporate to obtain a concentrated solution, wherein the concentration of fusidic acid in the concentrated solution is 50000 μg / ml, and the volume ratio of ethanol to fusidic acid fermentation broth is 0.8:1; ...

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Abstract

The invention discloses a method for extracting and purifying fusidic acid. The method comprises the following steps: S1) preparing dry mycelia: separating fusidic acid fermentation liquor, taking mycelia and drying, thereby acquiring dry mycelia; S2) preparing a concentrated solution using an organic solvent for soaking the dry mycelia acquired in the step S1), taking an organic solvent and concentrating, thereby acquiring the concentrated solution; S3) preparing fusidic acid: extracting, decolorizing, concentrating for crystallizing and drying the concentrated solution acquired in the step S2), thereby acquiring a crude product of fusidic acid; re-crystallizing and drying the crude product of fusidic acid, thereby acquiring fusidic acid. According to the invention, the dosage of organicsolvent and extraction agent can be greatly reduced and the cost for extracting and purifying fusidic acid can be lowered; the organic solvent and extraction agent can be recycled, so as to protect environment; the method disclosed by the invention is stable in process, simple and convenient in operation and suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of chemical substance extraction, in particular to a method for extracting and purifying fusidic acid. Background technique [0002] Fusidic acid (Fusidic Acid, CAS6990-06-3) is a white crystal (powder), almost insoluble in water, soluble in organic solvents such as benzene, methanol, ethanol, ethyl acetate, etc. It is extracted and separated from the mycelia (intracellular) of the fermentation broth of the fungus Estercocci. This substance is the compound with the most antibacterial activity among the fusidanes, and it is mainly effective against Gram-positive bacteria, especially Staphylococcus. Antibacterial activity, including high sensitivity to Staphylococcus aureus, coagulase-negative bacteria, Staphylococcus aureus, Clostridium, Corynebacterium, etc. [Collignon P, TurndgeJ. International journal of antimicrobial agents, 1999,12 :45-48]. Since the end of the 1980s, staphylococcus infections have inc...

Claims

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Application Information

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IPC IPC(8): C07J13/00
CPCC07J13/007
Inventor 攸德伟徐海
Owner 宣城菁科生物科技有限公司
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