Novel sperm quality evaluation method

Abstract

The invention discloses a novel sperm quality evaluation method. According to the method, large-visual-field multi-target tracking positioning detection on a living sperm sample is realized by means of a low-magnifying-power large-visual-field microscopic imaging system; high-resolution form imaging to the living sperm sample is realized by means of a small-visual-field high-resolution microscopicimaging system. In combination of two microscopic imaging systems, through quick scanning of an imaging area of a small-visual-field high-resolution imaging system in a large-visual-field imaging area, high-resolution form imaging is performed on total target living sperms which are selected in the large-visual-field imaging area, and activity detection and high-resolution form detection on the living sperm sample in a large-visual-field are finally realized. The novel sperm quality evaluation method is particularly suitable for high-resolution form analysis of a large number of moving spermsin a large visual field in human sperm inspection and can simultaneously detect the indexes such as sperm activity and form of the no-dyeing no-mark seminal fluid sample.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging for semen examination, and in particular relates to a novel sperm quality evaluation method. Background technique [0002] As a key part of human semen examination, sperm motility and morphology are of great significance to reproductive and developmental research, especially to clinical reproductive medicine. Existing medical clinical testing uses different sample preparation and microscopic detection methods to detect the concentration, vitality and morphology of sperm respectively. [0003] Sperm motility can be done manually or computer-aided, and computer-aided sperm analysis technology can provide quantitative parameters of sperm motility with high accuracy, which is conducive to the standardization and standardization of semen examination. Computer-aided sperm analysis technology uses low-magnification microscopic imaging system, image acquisition, processing and analysis system...

AI Technical Summary

Problems solved by technology

In addition, since the sperm motility detection and morphological analysis are performed separately, it is impossible to obtain the corresponding relationship between sperm motility and morphology, which are crucial for sperm quality analysis

[0004] The movement speed of sperm is extremely fast, the speed is tens of microns per second. To ensure the continuous observation of moving sperm and the tracking of hundreds of targets, large-field imaging is required; while the head size of sperm is 3 to 5 microns, and the morphological structures such as vesicles are in the number On the order of hundreds of nanometers, while the limit resolution of low-magnification imaging is at the micron level, clear imaging cannot be achieved, and the shape of the head cannot be accurately measured and analyzed

[0005] Limited by the existing optical imaging design, the field of view of imaging is inversely proportional to the magnification of the objective lens. Low magnification imaging can achieve a large field of view but cannot achieve high resolution. High magnification imaging can achieve high resolution and cannot directly achieve a large field of view.

Although for non-moving samples, large field of view and high resolution imaging can be performed by methods such as imaging field of view stitching, but for fast moving objects such as sperm, simple field of view stitching is not suitable, which will cause the movement of sperm. omission or duplication

The effective imaging area is limited by the microscopic imaging detection element and optical design at the same time, and the effective imaging area of ​​the microscopic image acquisition element is limited by technology and cost

Images