Application of enterobacter hormaechei K20 to degrading agricultural wastes

A technology for Enterobacter horii and agricultural waste, applied in the field of Enterobacter horii K20 and its degradation of agricultural waste, can solve the problem of low enzyme activity, achieve stable enzyme activity, strong adaptability, and significant lignin degradation ability Effect

Active Publication Date: 2019-04-02
HAILIN ZHONGNONG GUOTAI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • Application of enterobacter hormaechei K20 to degrading agricultural wastes
  • Application of enterobacter hormaechei K20 to degrading agricultural wastes
  • Application of enterobacter hormaechei K20 to degrading agricultural wastes

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specific Embodiment approach 1

[0025] Specific embodiment 1: A lignocellulosic substance degrading bacterium K20 of this embodiment, it is Enterobacter hormaechei (Enterobacter hormaechei) K20, preserved in the general microorganism center of China Microorganism Culture Collection Management Committee, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, with a preservation date of December 12, 2014, and a preservation number of CGMCC No.10163.

[0026] Enterobacter hormaechei (Enterobacter hormaechei) K20 of the present embodiment is a Gram-negative bacterium, and the bacterial cell shape of this strain is rod-shaped, and the bacterial cell size is 0.51~0.57×1.2~1.5 μm, does not form spores, and has no flagella and pods Membrane; forms round, opaque, milky-white colonies with smooth protrusions and neat edges on LB medium (such as figure 1 shown).

[0027] The results of physiological and biochemical reactions of the strain consisted of 18 items a...

specific Embodiment approach 2

[0086] Specific implementation mode two: the application of a lignocellulosic substance-degrading bacteria K20 in this embodiment, which is used for degrading agricultural waste, especially for degrading lignocellulosic substances in bagged fungus fertilizer and corn stalks .

[0087] Bacterial strain of the present invention is carried out following functional detection:

[0088] Enterobacter hormaechei (Enterobacter hormaechei) K20 of the specific embodiment was carried out to measure the weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0089] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0090] 1.1 Corn stalk powder

[0091] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh ...

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Abstract

The invention relates to an application of enterobacter hormaechei K20 to degrading agricultural wastes. The enterobacter hormaechei ( Enterobacter hormaechei ) K20 is preserved in China General Microbiological Culture Collection Center, at 12 December 2014, wherein the preservation number is CGMCC No.10163. Bacterial strains are proliferated, the adaptability is high, and the application is broad. The weight loss rate of bagged fungus waste after fermentation treatment with the enterobacter hormaechei ( Enterobacter hormaechei ) K20 is 23% or above, and the weight loss rate of corn straw after fermentation treatment with the enterobacter hormaechei ( Enterobacter hormaechei ) K20 is 27% or above.

Description

[0001] This application is a divisional application of the following patent application, the application number of the original application: 201510861983.2, the application date: 2015-12-01, the name of the invention: a strain of highly efficient degrading lignocellulosic substances K20 and its application. technical field [0002] The invention relates to a strain of Enterobacter hallii K20 and its use for degrading agricultural waste. Background technique [0003] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become the largest in China. source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01
Inventor 丁仕波王鲲鹏杨峰山孙永帅吴奇杨微刘春光王庆华魏才强曲金玲张慧于文全
Owner HAILIN ZHONGNONG GUOTAI BIOTECHNOLOGY CO LTD
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