Reagent and method capable of identifying and detecting babesia sp. and babesia motasi of sheep

Abstract

The invention discloses a reagent and method capable of identifying and detecting babesia sp. and babesia motasi of sheep. The reagent disclosed by the invention comprises five artificial sequences ofSEQID No.1, SEQID No.2, SEQID No.3, SEQID No.4 and SEQID No.5. Nested PCRs are used for the first time, multiplex PCRs are combined, the advantages of two PCRs are concentrated in one method, and technique optimization is performed, so that shortcomings of two methods are overcome, and a reaction system in a PCR apparatus is completed by a one-step method and through two reaction courses. The shortcomings that by a traditional aetiology detection method, the kind of babesia is difficult to distinguish, the sensibility is low, the operation is tedious and the detection cost is high, and the reagent has the advantages of being high in detection speed, high in specificity, sensitivity and repeatability and simple in use steps, and is suitable for identification and detection of the babesia of the sheep in bodies of clinical animals or propagation mediums of ticks in production practice and large-scale molecular epidemiology investigation.

Description

technical field [0001] The invention relates to a reagent for differentiating and detecting Babesia sheep undetermined species and Babesia mosoni, and a method for differentiating and detecting Babesia sheep undetermined species and Babesia mosoni for non-disease detection purposes. Background technique [0002] Babesia is a kind of parasitic animal and human erythrocytes belonging to Apicomplexa (Apicomplexa), Sporozoea (Sporozoea), Piroplasmida (Piroplasmida), Babesiidae, Babesia ) tick-borne blood protozoa. So far, there are more than 100 species of Babesia reported in the world, and the currently reported and named Babesia infecting sheep is mainly Babesia mosoni ( B. motasi ), Babesia sheep ( B.ovis ), B. crassa, B. foliata and B. taylori. The vectors of Babesia ovis and Babesia mosenii are Rhizocephalus ticks ( Rhipicephalus ) and blood ticks ( Haemaphysalis ) species of ticks, the vectors of other Babesia are unknown. The biological characteristics of differ...

AI Technical Summary

Problems solved by technology

However, in the infestation period and early infection stage, due to the low infection rate, it is difficult to find the parasites in the blood smears. In addition, the shapes of various Babesia are relatively similar, and it is easy to cause parasites in the process of smear making and staining. deformation, so it is often difficult to identify the species of Babesia ovis, and it is difficult for those who have not been engaged in the research of the disease for a long time to confirm what kind of Babesia it is

Serological detection methods represented by indirect fluorescent antibody test (IFTA) and ELISA can only detect serum antibodies and cannot reflect the real-time infection status of the host, nor are they suitable for the detection of vector tick infection

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