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Test strip for simultaneously detecting influenza A and B viruses and preparation method

A type of influenza B virus, type A technology, applied in the field of medical testing, can solve the problems of inability to guarantee the quality of the test unit, poor sensitivity and repeatability, and affect the test results, so as to facilitate clinical diagnosis, improve stability and sensitivity. , the effect of easy operation

Inactive Publication Date: 2019-04-05
SHENZHEN YILIFANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Each test unit of POCT is independent, so the quality of each test unit cannot be guaranteed to be the same
Based on immunochromatographic test strips and instruments, the test results will be affected by the difference in temperature, humidity and pH value
The methodological flaws of some POCT instruments, such as poor sensitivity and repeatability, and narrow linear range, are only used for reference in emergencies or emergencies, and need to be sent to the laboratory for re-examination if necessary.

Method used

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  • Test strip for simultaneously detecting influenza A and B viruses and preparation method
  • Test strip for simultaneously detecting influenza A and B viruses and preparation method
  • Test strip for simultaneously detecting influenza A and B viruses and preparation method

Examples

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preparation example Construction

[0047] A preparation method for preparing the time-resolved immunochromatographic test strips for the detection of influenza A and B viruses, comprising the following steps:

[0048] (1) respectively immobilizing the monoclonal capture antibody of type A and type B influenza virus and goat anti-mouse IgG antibody recognizing a single antigenic epitope on the reaction membrane 3, forming the first detection area, the second detection area and the control area;

[0049] (2) Prepare time-resolved fluorescent microsphere-labeled monoclonal detection antibodies for influenza A and B viruses, and use the prepared time-resolved fluorescent microsphere-labeled monoclonal detection antibodies for influenza A and B viruses in a ratio of 1:1 Mix and spray on bonding pad 2;

[0050] (3) Paste the sample pad 1, the binding pad 2, the reaction membrane 3 and the water absorption pad 4 on the bottom plate 5 in sequence to obtain the time-resolved fluorescence immunochromatography test strip....

Embodiment 1

[0075] Example 1 Time-resolved immunochromatographic test strip for simultaneous detection of influenza A and B viruses.

[0076] A time-resolved immunochromatographic test strip for detecting influenza A and B viruses in this embodiment includes a bottom plate, and a sample pad, a binding pad, a reaction membrane and a water absorption pad sequentially arranged on the bottom plate. The binding pad is coated with an equivalent number of fluorescent microsphere-labeled influenza A and B influenza virus monoclonal detection antibodies, and the reaction membrane includes a first detection zone T1 and a second detection zone T2 that are arranged in parallel and spaced 2mm and 4mm apart from each other. and the control area, the first detection area T1 and the second detection area T2 are close to the binding pad, the control area is close to the water-absorbing pad, and the first detection area T1 and the second detection area T2 are respectively coated with Influenza A and B viru...

Embodiment 2

[0086] Example 2 Time-resolved immunochromatography kit for simultaneous detection of influenza A and B viruses.

[0087] The time-resolved fluorescence immunochromatography kit for simultaneous detection of influenza A and B viruses in this embodiment includes: the test strip described in Embodiment 1, a plastic cartridge, and a sample buffer.

[0088] In this example, the sample buffer contains 0.5% BSA, 0.05% Tween-20 in PBS pH7.4 buffer solution.

[0089] When using the time-resolved fluorescence immunochromatography kit for simultaneous detection of influenza A and B viruses of the present invention, the throat swab sample to be tested is added to the sample buffer, 50 μL of sample diluent is dropped on the sample pad of Capillary action transports the sample to the binding pad. When the throat swab sample contains influenza A and B viruses, it forms an antigen-antibody complex with the monoclonal antibody on the fluorescent microspheres. With the chromatographic action,...

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Abstract

The invention discloses a time-resolved fluorescent immunochromatographic test strip for simultaneously detecting influenza A and B viruses and a preparation method. The test strip comprises a bottomplate, and a sample pad, a bonding pad, a reaction film and an absorbent pad which are sequentially adhered to the bottom plate. The binding pad is coated with influenza A and B viruses monoclonal detection antibodies marked with time-resolved fluorescent microspheres. The reaction film comprises a first detection zone, a second detection zone, and a control zone that are disposed in parallel andspaced apart from each other. The first detection zone is close to the bonding pad. The control area is close to the absorbent pad. The second detection zone is located in the middle of the first detection zone and the control zone. The first detection zone, the second detection zone, and the control zone are respectively coated with influenza A and B viruses monoclonal capture antibodies and goatanti mouse IgG antibodies that recognize a single antigenic epitope. The time-resolved fluorescent immunochromatographic test strip for simultaneously detecting influenza A and B viruses has high sensitivity, simple operation, low cost and short detection time, which can effectively meet the needs of clinical rapid inspection.

Description

technical field [0001] The invention relates to medical testing technology, in particular to a time-resolved fluorescent immunochromatographic test strip for simultaneous detection of influenza A and B viruses and a preparation method. Background technique [0002] Influenza (flu) is an acute respiratory infection caused by influenza virus, and is a highly contagious and fast-spreading disease. It is mainly spread through airborne droplets, person-to-person contact or contact with contaminated objects. Typical clinical symptoms are: sudden onset of high fever, general pain, significant fatigue and mild respiratory symptoms. Generally, the autumn and winter seasons are the high incidence period, and the resulting complications and death are very serious. The disease is caused by influenza virus, which can be divided into three types: A, B, and C. Among them, the influenza caused by type A is the most widespread and severe, type B often causes outbreaks, and type C mostly ca...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/577G01N33/533G01N21/64
CPCG01N21/6408G01N21/6428G01N33/533G01N33/558G01N33/56983G01N33/577
Inventor 胡涛廖生赟陈建东张贵
Owner SHENZHEN YILIFANG BIOTECH CO LTD
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