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Insect bioprotein extract, and preparation method and application thereof

A biological protein and extraction method technology, applied in the field of natural active ingredient extraction, can solve the problems of increasing post-processing production costs, high requirements for reaction equipment, and increased non-protein content of extracts, so as to relieve and treat gout symptoms and reduce uric acid. Good, good effect of lowering uric acid

Inactive Publication Date: 2019-04-09
中商国能孵化器集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, although the extraction rate of alkaline extraction is high, the extraction is simple and easy, and the production cost is low, under the condition of high alkali, lys-alanine will be generated in the protein extraction solution, which is not only poisonous, but also Causes the loss of nutrients; and under high alkali conditions, it will also cause protein hydrolysis, and accelerate the Maillard reaction, resulting in dark brown substances, which will cause the color of the extract to deepen, the non-protein content in the extract will increase, and the separation effect will decrease.
In addition, the existing alkaline and salt extraction methods have high requirements for reaction equipment, and will cause serious environmental pollution and increase the production cost of post-processing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The insect biological protein extract described in this embodiment is extracted and prepared according to the following steps:

[0024] (1) Take gecko, scorpion and centipede as insect raw materials according to the mass ratio of 0.5:3:0.1, mix and pulverize after conventional sieving and dust removal, and set aside;

[0025] (2) Soak the above-mentioned mixed insect raw material in water accounting for 3 times the weight of the total insect raw material, and extract at 60°C for 3 hours. After the extract is separated from solid and liquid, collect the water extract and precipitate respectively thing;

[0026] (3) Take the collected precipitate and control the CO 2 The flow rate is 50L / min, and the ethanol solution with a mass concentration of 50wt% is used as an entrainer to carry out supercritical extraction for 2 hours. , to obtain the extract;

[0027] (4) Combine the above aqueous extracts and extracts, concentrate and freeze-dry for 4 hours to obtain the desire...

Embodiment 2

[0029] The insect biological protein extract described in this embodiment is extracted and prepared according to the following steps:

[0030] (1) Take gecko, scorpion and centipede as insect raw materials according to the mass ratio of 1.5:1:1, mix and pulverize after conventional sieving and dedusting, and set aside;

[0031] (2) Soak the above-mentioned mixed insect raw material in water accounting for 8 times the weight of the total insect raw material, and extract at 100°C for 2 hours. After the extract is separated from solid and liquid, collect the water extract and precipitate respectively thing;

[0032] (3) Take the collected precipitate and control the CO 2 The flow rate is 150L / min, and the ethanol solution with a mass concentration of 70wt% is used as an entrainer for supercritical extraction for 2 hours, and the amount of the entrainer is controlled to be 50wt% of the weight of the precipitate. After collecting the extract and recovering ethanol by distillation ...

Embodiment 3

[0035] The insect biological protein extract described in this embodiment is extracted and prepared according to the following steps:

[0036] (1) Take gecko, scorpion and centipede as insect raw materials according to the mass ratio of 1:2:0.5, mix and pulverize after conventional sieving and dust removal, and set aside;

[0037] (2) Soak the above-mentioned mixed insect raw material in water accounting for 5 times the weight of the total insect raw material, and extract at 80°C for 3 hours. After the extract is separated from solid and liquid, collect the water extract and precipitate respectively thing;

[0038] (3) Take the collected precipitate and control CO under the conditions of 25Mpa and 35°C 2 The flow rate is 100L / min, and the ethanol solution with a mass concentration of 95wt% is used as an entrainer for supercritical extraction for 2 hours. The amount of the entrainer is controlled to be 40wt% of the weight of the precipitate, and the extract is collected and th...

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PUM

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Abstract

The invention belongs to the technical field of natural active ingredient extraction, particularly relates to an insect bioprotein extract and further discloses a preparation method and an applicationof the insect bioprotein extract. The insect bioprotein extract takes a gecko, a scorpion and a centipede as insect raw materials; the obtained extract has a better uric acid reduction effect, can effectively alleviate and treat a gout symptom and can be used for preparing a pharmaceutical preparation for treating gout. According to the insect bioprotein extract, active ingredients of the insectprotein are extracted by a combined water extraction and CO2 supercritical extraction manner; compared with the conventional water extraction or alcohol extraction manner in the prior art, an active ingredient dissolution rate of the prepared extract is higher; a raw material utilization ratio is further increased; and the extract has the better uric acid reduction effect.

Description

technical field [0001] The invention belongs to the technical field of extracting natural active ingredients, in particular relates to an insect biological protein extract, and further discloses its preparation method and application. Background technique [0002] According to statistics, there are more than 1 million species of insects in the world, of which more than 3,650 species are known to be edible. Insects have the characteristics of many types, large numbers, wide distribution, and rapid reproduction. Insects contain high protein, low fat, and low cholesterol, and have the advantages of reasonable nutritional structure and less meat fiber. And attracted the attention of countries all over the world. Experts predict that by the 21st century, insects will become the third largest source of protein after microorganisms and cell organisms. [0003] According to reports, the protein content of insects is higher than that of beef, pork, chicken, and fish. For example, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14A61K38/02A61P19/06
CPCA61K38/02A61P19/06C07K1/145Y02P20/54
Inventor 王海平
Owner 中商国能孵化器集团有限公司
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