KASP (competitive allele specific PCR) molecular marker and method for detecting low-amylose content gene Wx<mq> of rice

Abstract

The invention discloses a KASP (competitive allele specific PCR) molecular marker and a method for detecting a low-amylose content gene Wx<mq> of rice. The molecular marker is designed according to mutation from G to A in 497 bp position of the fourth exon of the Wx<mq> gene and named Wx<mq>-KASP. KASP marker primers comprise two forward specific primers and one reverse universal primer. Genetic typing is performed according to the KASP marker, enzyme digestion and electrophoresis are not used after PCR amplification, high-throughput detection of multiple samples can be realized, the distribution condition of Wx<mq> alleles in rice segregation population can be detected quickly, the result accuracy is good, and compared with conventional molecular markers, the KASP molecular marker has theadvantages of being rapid, convenient, lower in cost and the like. The KASP molecular marker has great significance in molecular marker-assisted selection breeding of the low-amylose content gene Wx<mq> of rice.

Description

technical field [0001] The invention relates to a method for detecting rice low amylose content gene Wx mq The KASP molecular marker and method belong to the technical field of rice breeding. Background technique [0002] Rice is the main food crop in China. The annual planting area of ​​rice in China has remained at about 30 million hectares, and the total rice production ranks first in the world. China is also the world's largest rice consumer, with more than 60 percent of the population taking rice as a staple food. For a long time, high yield has been the primary indicator of rice breeding. With the improvement of people's living standards, the demand for high-quality rice is increasing day by day. Rice quality, especially eating quality, has become the primary consideration for consumers and breeders. 90% of rice is composed of starch, and starch is composed of amylose and amylopectin, so the amylose content is the most important factor affecting the eating quality of...

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Problems solved by technology

Wang et al (Wang C L, Zhang Y D, Zhu Z, Chen T, Zhao L, Lin J, Zhou L H.2009. Development of a new japonica rice variety Nan-jing 46 with good eating quality by marker assisted selection. Molecular Plant Breeding, 7( 6): 1070-1076) using Wx mq Gene developed a CAPS marker, which was successfully applied to molecular marker-assisted selection breeding. However, the use of CAPS markers requires enzyme digestion and electrophoresis of PCR amplification products. During the rice breeding process, breeders need to screen a large number of progeny materials, so the cost high and time consuming

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