PUFA soft capsule containing sialic acid and preparation method thereof
A technology of sialic acid and soft capsule, applied in the field of PUFA soft capsule containing sialic acid and its preparation, can solve the problems such as no DHA soft capsule, and achieve the effects of being suitable for popularization and use, portable products and easy-to-obtain materials
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0058] The present embodiment provides a PUFA oil suspension containing sialic acid and a preparation method thereof, wherein the PUFA oil is DHA oil; the specific steps are as follows:
[0059] 1) Get 20g of sialic acid powder, mix with 160g of DHA oil, and shear to fully disperse under 17000rpm conditions;
[0060] After dispersion, take different parts of the oil suspension for measurement, and the difference in sialic acid content is less than 3%;
[0061] 2) Take the dispersed sialic acid and DHA oil mixture in step 1) for high-speed grinding, under nitrogen protection, at a speed of 2000 r / min, with a screen gap of 0.4 mm, and grind for 30 mins.
[0062] In this example, the sialic acid powder and DHA oil are all commercially available; the sialic acid content in the sialic acid powder is >98%, and the DHA content in the DHA oil is ≥35%.
Embodiment 2
[0064] The present embodiment provides a PUFA oil suspension containing sialic acid and a preparation method thereof, and the PUFA oil is DHA oil; the difference from Example 1 is only:
[0065] The sialic acid powder adopted in this embodiment is prepared by the following method:
[0066] Taking Escherichia coli as the fermentation cell, the preservation number CCTCC NO: M 201810, the seed liquid was added to the fermenter containing the sterilized fermentation medium at an inoculum of 1%, and the LB medium contained: peptone 10g / L, yeast Extract powder 5g / L, sodium chloride 10g / L, pH 7.0, fermented at 37°C, supplemented with glucose at a rate of 2.5g / (L*h) by means of stream feeding, continuous After 75 hours of fermentation, put the tank in a tank to obtain a fermentation liquid containing sialic acid. The fermentation liquid is separated from the bacterial residue by membrane filtration, and the liquid obtained by filtration is then subjected to sterilization, decolorizati...
Embodiment 3
[0070] The present embodiment provides a PUFA oil suspension containing sialic acid and a preparation method thereof, and the PUFA oil is DHA oil; the difference from Example 2 is only:
[0071] The DHA oil adopted in the present embodiment is prepared by the following method:
[0072] Taking Schizochytrium as the fermentation cell, the seed liquid is added to the fermentation tank containing the fermentation medium after steam sterilization, and the formula of the fermentation medium is as follows: glucose 100g / L, yeast extract 6g / L, glutamate Sodium 20g / L, Sodium Chloride 3g / L, Potassium Dihydrogen Phosphate 2g / L, Magnesium Sulfate 5g / L, Calcium Chloride 1g / L, Sodium Bicarbonate 0.5g / L, Sodium Sulfate 7g / L, Ammonium Sulfate 8g / L, potassium chloride 0.6g / L, natural pH; control the ratio of seed liquid to fermentation medium to 5% and the ratio of dissolved oxygen to 8% during the fermentation process, put it in a tank after 100h fermentation at 28°C, centrifuge or filter, and...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More