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Application of smxp-58 strain in reducing cellulose content of tobacco stem

A technology of SMXP-58 and cellulose content, applied in the fields of application, tobacco, and processed tobacco, etc.

Active Publication Date: 2021-11-19
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to some obvious differences in the specific material structure and material content between tobacco stems and tobacco leaves, there is a certain degree of uncertainty whether the degradation technology in tobacco leaves can be used in tobacco stems in the past, and what the technical effect will be after specific applications. of

Method used

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  • Application of smxp-58 strain in reducing cellulose content of tobacco stem
  • Application of smxp-58 strain in reducing cellulose content of tobacco stem
  • Application of smxp-58 strain in reducing cellulose content of tobacco stem

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In order to use the SMXP-58 strain to degrade cellulose in tobacco stems, it is first necessary to prepare the strain into an easy-to-use bacterial agent, and the specific preparation process is as follows.

[0022] (1) Activated strains: put the SMXP-58 strain on the LB plate, and activate it in a biochemical climate chamber at 37°C for 1 day;

[0023] (2) Preparation of seed liquid: Scrape the bacteria cultured in step (1) in the ultra-clean workbench, inoculate in a 18×180 test tube containing 5 mL of liquid LB medium, and place on a shaker at 30°C and 150 rpm Overnight culture;

[0024] (3) Expansion culture: Transfer the seed solution in step (2) to a sterile 250mL Erlenmeyer flask containing 100mL liquid LB medium at the ratio of 1 mL bacterial suspension / 100 ml LB medium (1% volume ratio) medium, 30°C, 150 rpm, cultured to OD 600 = about 2.0;

[0025] (4) Preparation of bacterial agent: Centrifuge the bacterial solution in step (3) at 3600 rpm for 10 minutes, ...

Embodiment 2

[0027] When utilizing the bacterial agent prepared in the embodiment 1 to process the tobacco stem, the concrete steps are:

[0028] Take the cut stems for testing on the silk production line, the parameters of the cut stem samples are: the average thickness is 0.16 mm, the average length is 3.5 mm, and then evenly divided into multiple parts, each 100 g;

[0029] Spray the microbial agent prepared in Example 1 evenly on the surface of the cut stems with a sprayer, and then adjust the moisture content of the cut stems to 25%.

[0030] In order to obtain the optimal action conditions of the bacterial preparation, the temperature, humidity, and time of the bacterial preparation were used as influencing factors respectively, and a 3-factor 3-level orthogonal experimental design was carried out (horizontal factor table (L9(3) 3 )), the specific design is shown in Table 1:

[0031] Table 1 Orthogonal test factor level L 9 (3 3 )

[0032]

[0033] Note: A is the action temper...

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Abstract

The invention belongs to the technical field of cigarette raw material production and processing, and specifically relates to a patent application for the application of a specific strain of Bacillus subtilis SMXP‑58 in degrading cellulose in tobacco stems. In application, the Bacillus subtilis SMXP‑58 strain is prepared into a microbial agent containing live bacteria, which is mixed with tobacco stems and then fermented to degrade the cellulose in the tobacco stems, thereby reducing the cellulose content in the tobacco stems. This application belongs to the new use development of existing known bacterial strains. Preliminary research results show that Bacillus subtilis SMXP‑58 can not only reduce the pectin content in tobacco stems, but also reduce the cellulose content in tobacco stems in a short period of time, and the degradation rate of cellulose in tobacco stems It can reach more than 30%, showing a better technical effect of reducing the cellulose content in tobacco stems. At the same time, this application also provides good reference and application demonstration for the development of new uses of existing microbial strains, and has good practical value.

Description

technical field [0001] The invention belongs to the technical field of production and processing of cigarette raw materials, and specifically relates to a patent application for the application of a specific strain of Bacillus subtilis SMXP-58 in degrading cellulose in tobacco stems. Background technique [0002] Tobacco stems are the thick and hard veins of tobacco leaves, accounting for about 25% of tobacco leaves, and are the main raw materials for making shredded stems that play a supporting role in cigarettes. In the early days of cigarette production, it was generally believed that tobacco stems had more impurities and poor smokeability after burning, so they were only used in the production of low-grade cigarettes. However, with the update of cigarette preparation technology and health awareness, in the production of existing cigarettes, it is generally believed that the application of appropriate content of cut stems can not only improve the filling performance of sh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 杨宗灿张婷婷冯颖杰张展许衡李家美彭玉富申洪涛陈伟
Owner CHINA TOBACCO HENAN IND
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