A screening method for germplasm of spring rapeseed with high light efficiency
A screening method, high light efficiency technology, applied in horticultural methods, botanical equipment and methods, complex mathematical operations, etc.
Active Publication Date: 2022-02-11
陕西省杂交油菜研究中心
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Problems solved by technology
It is becoming more and more difficult to greatly increase the yield level of rapeseed varieties simply by using heterosis; various ways to increase crop yield in production have played a role, but this yield "bottleneck" still exists
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Embodiment 1
[0058] The instruments used in this embodiment include: LI-6400XT photosynthesis measurement system; the net photosynthetic rate, Changes in photosynthetic parameters such as stomatal conductance and environmental factors such as photosynthetically active radiation and atmospheric temperature; measure the light response curve of rapeseed, CO 2 response curve;
[0059] 1. Determination of photosynthetic gas exchange parameters:
[0060] (1) Blades:
[0061] ① Sampling location: select long-stalked leaves at the 6th-8th leaf position at the seedling stage, short-stalked leaves at the 1st and 2nd leaf positions and sessile leaves at the 2nd leaf position at the stalk and flowering stages;
[0062] ② Determination of the number of samples: select 12 leaves for each material;
[0063] ③ Sampling method: Cut the leaves from the petiole near the main stem, wrap the petiole with wet cotton wool immediately, and quickly test it on the machine (from taking leaves to measuring complet...
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The invention discloses a method for screening spring rapeseed germplasm with high light efficiency. Including: Determination of net photosynthetic rate of leaves and siliques, stomatal conductance, intercellular CO 2 Concentration, transpiration rate, stomatal limit value and water use efficiency; determination of photosynthetic area of rapeseed silique peel; determination of light response curve, CO 2 Response curve; 1) The net photosynthetic rate of the long-stalked leaves, short-stalked leaves, sessile leaves and siliques of the candidate material and the control variety "Qingza 7" were compared at the seedling stage, stalk stage, flowering stage and silique stage. Calculate the average value after comparison, and the ones that are 5% higher than the control are primary high-light-efficiency materials; 2) measure the light response curve and CO 2 curve; use the light response curve to calculate the light saturation point, light compensation point and light quantum efficiency, and use the CO 2 The curve calculates the carboxylation efficiency, CO 2 Compensation point and CO 2 saturation point; will have at least a high light saturation point or low CO 2 The germplasm of the compensation point is used as the final high-efficiency material.
Description
technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for screening spring rape germplasm with high light efficiency. Background technique [0002] High photoefficiency breeding is a method of screening low photorespiration type plants to breed crop varieties. The strength of photorespiration is often expressed by the level of carbon dioxide compensation point. The higher the intensity of photorespiration, the higher the carbon dioxide compensation point, the lower the intensity of photosynthesis, and the lower the photosynthetic efficiency; otherwise, the higher the photosynthetic efficiency. Low-light efficiency plants can be transformed into high-light-efficiency plants by hybridization, radiation induction, etc.; or individual plants or lines with low or low compensation points can be selected from low-light-efficiency plants to breed new varieties with high light-efficiency. [0003] Photosynthesis is t...
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IPC IPC(8): A01G22/15A01G7/02A01H1/04G06F17/18
CPCA01G7/02A01G22/15A01H1/04G06F17/18Y02A10/40
Inventor 赵小光张耀文陈文杰赵兴忠王竹云侯君利王丽萍张新
Owner 陕西省杂交油菜研究中心