Penicillium bilaiae capable of preventing sesame blight and promoting and guiding resistance and screening method and application thereof

A technology of sesame fusarium wilt and Penicillium bairui, applied in the field of microorganisms

Active Publication Date: 2019-04-16
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Penicillium bilaiae capable of preventing sesame blight and promoting and guiding resistance and screening method and application thereof
  • Penicillium bilaiae capable of preventing sesame blight and promoting and guiding resistance and screening method and application thereof
  • Penicillium bilaiae capable of preventing sesame blight and promoting and guiding resistance and screening method and application thereof

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Embodiment 1

[0039] Embodiment 1, the separation and the screening of soil microorganism

[0040] From the soil sample in Shigou Village, Xicun Township, Mianchi County, Sanmenxia City, weigh 1g of soil that was dried at room temperature, grind and sieve, add 10mL of sterile water to fully shake to obtain a soil suspension, and then shake the soil suspension to make a gradient For dilution, take 100 μL of different gradient dilutions and spread them on the Montjara red medium, and incubate them upside down in a constant temperature incubator at 28°C for 48 hours, until the colonies grow, and pick colonies with different sizes, colors or shapes on the PDA medium separation and purification.

[0041] Screening of bio-control bacteria by plate confrontation method: inoculate Fusarium oxysporum on PDA medium, culture at 28°C for 5-10 days, use a sterile puncher to punch out a circular mycelium block with a diameter of 5 mm, and inoculate it on the PDA medium. In the center of the new PDA medi...

Embodiment 2

[0046] Embodiment 2, the inhibitory effect of bacterial strain 47M-1 to other main disease pathogenic bacteria on sesame

[0047] According to the method described in embodiment 1, measure the inhibitory action of bacterial strain 47M-1 to the pathogenic bacterium of other main diseases on sesame, the result is as table 2, in flat plate confrontation test, bacterial strain 47M-1 is to Sesame bean shell coccus (Macrophominaphaseolina) , Fusarium solani (Fusarium solani), Bipolaris sorokiniana (Bipolaris sorokiniana), Corynespora cassiicola (Corynespora cassiicola) and Rhizoctonia solani (Rhizoctonia solani) all have a diameter of inhibition zone above 20 mm; In the drug-containing plate test, except for Corynesium polybasicum, other pathogenic bacteria were strongly inhibited. It shows that the isolate 47M-1 has a broad-spectrum antibacterial activity against the pathogens of sesame diseases.

[0048] Table 2 Inhibitory effect of strain 47M-1 and its fermentation filtrate on o...

Embodiment 3

[0051] Embodiment 3, identification of bacterial strain 47M-1

[0052] The activated strain 47M-1 was transferred to the morphological identification medium, placed in an incubator at 28°C, and cultured in the dark for 7 days, and the culture characteristics of the strain 47M-1 on the four morphological mediums were observed and recorded. Extract the genomic DNA of 47M-1, use universal primers to amplify the sequences of its ITS and Calmodulin (calmodulin) genes, compare the sequencing results on NCBI, and select the sequences of the model strains with high similarity to establish a phylogenetic tree .

[0053] Morphological characteristics of 47M-1: Colony morphology of strain 47M-1 on G25N: the colony has radiation grooves, mild sporulation, gray-green colony, velvet, white edge of 3-4mm, no secretion droplets, no soluble pigment , flesh pink on the back, light pink on the edge. Colony morphology of strain 47M-1 on CYA: the colony has radiation grooves, moderate sporulatio...

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Abstract

The invention discloses a penicillium bilaiae capable of preventing sesame blight and promoting and guiding resistance and a screening method and application thereof. The penicillium bilaiae is penicillium bilaiae 47M-1 (Penicillium bilaiae) which is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University center for Collection, the preservation codeis CTCC M: 2018899, and the preservation data is December 17 , 2018. a strain has strong inhibition effect on sesame fusarium oxysporum, broad-spectrum bacterial inhibiting activity on pathogenicbacteria of other primary diseases of sesame is achieved, the growth of crops can be promoted to guide the crops to generate resistance, the penicillium bilaiae has no pathogenicity on the crops, common problems of chemical pesticides of the resistance to drugs, pesticide residues and environmental pollution are prevented, the penicillium bilaiae is healthy towards human and livestock and friendlyto the environment, and the demands on ecological agriculture of people are conformed.

Description

technical field [0001] The invention relates to a Penicillium belaii strain capable of preventing and controlling sesame wilt and having the effects of promoting growth and inducing resistance, a screening method and application thereof, and belongs to the technical field of microorganisms. Background technique [0002] Fusarium wilt caused by Fusarium is an important soil-borne disease distributed worldwide, which harms a variety of food crops and economic crops, and is called a cancer of plants. Fusarium oxysporum can infect the whole growth period of the plant, usually infecting the vascular system of the host plant transport tissue, causing various diseases such as root rot, stem rot, stem base rot, flower rot and ear rot of the plant , and produce toxins in the process of growth, development and metabolism to harm crops, cause crop wilting and death, and affect crop yield and quality. It is one of the most difficult and important diseases in production. [0003] At pre...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02A01N63/04A01P3/00A01P21/00C12R1/80
CPCC12N1/02C12N1/14A01N63/30C12R2001/80C12N1/145
Inventor 刘红彦赵新贝赵辉刘新涛倪云霞高素霞刘玉霞文艺王飞千慧敏何碧珀王婧
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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