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A pretreatment method for rapid detection of nitrofuran metabolites and its detection method

A nitrofuran and detection method technology, applied in the field of nitrofuran metabolite detection, can solve the problems of time-consuming and laborious, unsuitable for carrying the instrument, unsuitable for on-site operation, etc., so as to improve the operability, save the cost of the instrument, and shorten the time. Effect

Active Publication Date: 2021-02-09
广州食源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] It can be seen from the above: in the pretreatment step of the existing nitrofuran metabolite detection, after adding the extractant (ethyl acetate) for extraction, it needs to be equipped with nitrogen blowing instrument or solid phase extraction to remove ethyl acetate. These instruments are not suitable for Carry it with you, it is not suitable for on-site operation, and when there is too much oil in the sample, you need to add an organic solvent to remove the oil, which is time-consuming and laborious. Therefore, how to improve the pretreatment steps of nitrofuran metabolites to make it easier Fast, meeting the standards of on-site rapid detection is still challenging

Method used

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  • A pretreatment method for rapid detection of nitrofuran metabolites and its detection method
  • A pretreatment method for rapid detection of nitrofuran metabolites and its detection method
  • A pretreatment method for rapid detection of nitrofuran metabolites and its detection method

Examples

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Effect test

Embodiment 1

[0041] A pretreatment method for rapid detection of nitrofuran metabolites, comprising the steps of:

[0042] 1) Weigh 3.0 g of crushed fish meat in a centrifuge tube, add 5 mL of 5% trichloroacetic acid and 0.6 mL of 0.5% 2-nitrobenzaldehyde to obtain solution A, so that the pH of solution A=1.5, 2 -The mass percent concentration of nitrobenzaldehyde is 1‰;

[0043] 2) Heat solution A in a water bath at 80°C for 15 minutes, add 5 mL of ethyl acetate, mix thoroughly, and centrifuge at 4000 r / min for 5 minutes to obtain a supernatant;

[0044] 3) Take 5mL of supernatant, add 5mL of n-hexane and 0.5mL of phosphate buffer solution with pH=7.2 in sequence, shake up and down 50 times, let stand to separate layers, and the lower layer solution is the solution to be tested.

Embodiment 2

[0046] A pretreatment method for rapid detection of nitrofuran metabolites, comprising the steps of:

[0047] 1) Weigh 5.0 g of minced fish in a centrifuge tube, add 5 mL of 10% trichloroacetic acid and 0.6 mL of 2% 2-nitrobenzaldehyde in sequence to obtain solution A, so that the pH of solution A=0.8, 2 -The mass percent concentration of nitrobenzaldehyde is 2.4‰;

[0048] 2) Heat solution A in a water bath at 80°C for 20 minutes, add 3 mL of ethyl acetate, mix thoroughly, and centrifuge at 4000 r / min for 5 minutes to obtain a supernatant;

[0049] 3) Take 3mL of supernatant, add 8mL of n-hexane and 0.3mL of phosphate buffer solution with pH=7.2 in sequence, shake up and down 50 times, let stand to separate layers, and the lower layer solution is the solution to be tested.

Embodiment 3

[0051] A pretreatment method for rapid detection of nitrofuran metabolites, comprising the steps of:

[0052] 1) Weigh 10.0 g of crushed fish meat in a centrifuge tube, add 5 mL of 20% trichloroacetic acid and 0.6 mL of 3% 2-nitrobenzaldehyde in turn to obtain solution A, so that the pH of solution A=0.5, The mass percent concentration of 2-nitrobenzaldehyde is 1.8‰;

[0053] 2) Heat solution A in a water bath at 80°C for 10 minutes, add 10 mL of ethyl acetate, mix thoroughly, and centrifuge at 4000 r / min for 5 minutes to obtain a supernatant;

[0054] 3) Take 1mL of supernatant, add 10mL of n-hexane and 0.2mL of phosphate buffer solution with pH=7.2 in sequence, shake up and down 50 times, let stand to separate layers, and the lower layer solution is the solution to be tested.

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Abstract

The invention discloses a pretreatment method for rapid detection of nitrofuran metabolites and a detection method thereof. The method comprises the following steps: 1) sequentially adding an acid solution and a derivative reagent to the crushed animal tissue to obtain a solution A; 2) heating the solution A and adding an extractant for extraction to obtain a supernatant; 3) adding an extraction agent to the supernatant An organic solvent with a polarity ranging from 0 to 3.5 and a buffer solution are sequentially added into the liquid, and the detection liquid of the lower layer is taken for detection. After adding the extractant for extraction, the present invention can not only effectively remove ethyl acetate, but also remove excess oil in the sample, so that the substance to be detected is directly dissolved in the buffer solution, which is simple and efficient , without the need for additional instruments, greatly simplifies the operation steps, saves the cost of instruments, improves the operability of on-site detection, and also significantly shortens the pretreatment time, realizing the rapid on-site detection of nitrofuran metabolites.

Description

technical field [0001] The invention relates to the field of detection of nitrofuran metabolites, in particular to a pretreatment method for rapid detection of nitrofuran metabolites and a detection method thereof. Background technique [0002] Nitrofuran drugs are a class of synthetic broad-spectrum antibiotics with a 5-nitrofuran ring. There are four common types: furazolidone (AOZ), furaltadone (AMOZ), nitrofurazone (SEM) and nitrofurantoin (AHD ). my country's Ministry of Agriculture Announcement No. 235 "Maximum Residue Limits of Veterinary Drugs in Animal-derived Foods" stipulates that nitrofuran metabolites shall not be detected in animal-derived foods. The United States, Japan, South Korea and the European Union also stipulate that animal-derived It shall not be detected in food. But so far, there are still reports of nitrofurans and their metabolites detected in aquatic products. This not only endangers human health, but also affects the healthy development of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N33/558
CPCG01N1/28G01N33/558
Inventor 谢俊平李涛伍志权李慧琴彭程
Owner 广州食源生物科技有限公司
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