Low toxicity low irritation Commassie brilliant blue fast staining solution and dyeing and decoloring method

Abstract

The invention discloses a low toxicity low irritation Commassie brilliant blue fast staining solution and a dyeing and decoloring method. The low toxicity low irritation Commassie brilliant blue faststaining solution contains 0.005 percent-0.02 percent of Commassie brilliant blue, 1 percent -5 percent (v / v) of concentrated hydrochloric acid or 1 percent -5 percent (v / v) of 85% phosphate, 1 percent -5 percent (v / v) of glacial acetic acid, 1.5 per thousand-5 per thousand of anhydrous copper sulfate or chromium trichloride, and 3 percent -5 percent (v / v) of mixed water solution of absolute ethanol. The staining solution has low toxicity and low irritation, dyeing and decoloring time is short, and dyeing resolution and color fastness are high.

Description

technical field [0001] The invention relates to the technical fields of molecular biology technology and proteology, and mainly relates to a Coomassie brilliant blue rapid staining solution and a staining and decolorization method applied to protein polyacrylamide gel electrophoresis (SDS-PAGE). Background technique [0002] Polyacrylamide gel electrophoresis (sodium dodecyl sulfate polyacrylamide gelelectrophoresis, SDS-PAGE) has become an important technical means in modern biological research, and it is an economical, rapid and reproducible method for identifying the purity and content of proteins. SDS-PAGE electrophoresis is easy to operate and has a wide range of uses. It has become an important analytical technique in many research fields. SDS-PAGE includes four main steps, namely: gel preparation, electrophoresis, staining, and decolorization. Among them, gel preparation and electrophoresis have mature technology and special equipment. At present, the products commonl...

AI Technical Summary

Problems solved by technology

Among them, gel preparation and electrophoresis have mature technology and special equipment. At present, the products commonly used in the market for gel staining and decolorization mainly have the following defects: 1) the sensitivity and resolution are low, and the decolorization time is not easy to control. The results of gel staining after decolorization are not easy to preserve; 2) the silver nitrate staining method with higher sensitivity has complicated staining steps, the staining process is more polluted, and the cost of reagent raw materials used in staining is higher; 3) the fluorescent staining method and negative staining method The cost of dyeing reagents is high, and special instruments are required for detection and analysis, and the current popularity and utilization rate are low

Among them, Coomassie Brilliant Blue G250 is often used for protein quantitative detection due to its rapid binding reaction with proteins, and the formation of colored complexes is relatively stable and difficult to elute. Difficult to decolorize thoroughly, decolorization in water for a long time will easily cause the gel to absorb water and swell or even break; A large amount of volatilization of acetic acid will cause great harm to the human respiratory tract, etc.

Images