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A method for establishing a diagnostic model of acute myeloid leukemia and its application

A technology for acute myeloid cells and diagnostic models, applied in the field of establishing diagnostic models of acute myeloid leukemia, can solve the problems of lack of circulating cells, patient invasiveness, missed diagnosis, misdiagnosis, etc., to improve accuracy and atopy, avoid aggressive effect

Active Publication Date: 2021-08-27
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A major limitation of peripheral blood cell testing is that many patients have no detectable circulating cells at presentation, especially in the early stages of disease
As the "gold standard" for the diagnosis of acute myeloid leukemia, bone marrow biopsy is obviously invasive to patients, and the judgment of the results largely depends on the subjective experience of the examiner, which easily leads to missed diagnosis and misdiagnosis

Method used

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  • A method for establishing a diagnostic model of acute myeloid leukemia and its application
  • A method for establishing a diagnostic model of acute myeloid leukemia and its application
  • A method for establishing a diagnostic model of acute myeloid leukemia and its application

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Embodiment 1

[0032] The establishment method of the acute myeloid leukemia diagnostic model of the present embodiment comprises the following steps:

[0033] Step 1. Collection of serum samples: Collect serum samples from 40 cases of acute myeloid leukemia patients and 30 cases of healthy controls to obtain the serum samples of the marker discovery set, and then store them in a freezer at -80°C; The general information and clinicopathological characteristics of myeloid leukemia patients and healthy controls are shown in Table 1 below;

[0034] Table 1 General information and clinicopathological characteristics of acute myeloid leukemia patients and healthy controls in the marker discovery set

[0035]

[0036] *Indicates that the measurement data is described in the form of median (minimum value-maximum value)

[0037] Step 2. Analysis of serum samples: Take 100 μL of the serum samples of the marker discovery set frozen and thawed in step 1, and add 400 μL of L-2-chloroalanine containi...

Embodiment 2

[0049] The specific process of the method for diagnosis using the acute myeloid leukemia diagnostic model in this embodiment includes the following steps:

[0050] Step 1. Collection of serum samples: 15 patients with acute myeloid leukemia and 15 healthy controls were collected by venous blood collection to obtain the serum samples of the marker discovery set, and then stored at -80°C; The general information and clinicopathological characteristics of acute myeloid leukemia patients and healthy controls in the marker discovery set are shown in Table 3 below;

[0051] Table 3 General information and clinicopathological characteristics of acute myeloid leukemia patients and healthy controls in the marker validation set

[0052]

[0053] *Indicates that the measurement data is described in the form of median (minimum value-maximum value)

[0054] Step 2. Analysis of serum samples: Take 100 μL of the serum samples of the marker verification set frozen and thawed in step 1, an...

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Abstract

The invention discloses a method for establishing a diagnostic model of acute myeloid leukemia. The method comprises: 1. collecting serum; 2. analyzing and detecting the serum by ultra-high performance liquid chromatography and mass spectrometry; Serum spectra of patients and healthy controls to determine biomarkers; 4. Confirm the chemical information of biomarkers; 5. Confirm that glutamic acid, kynurenine, and oleic acid are the optimal biomarkers and establish a diagnostic model; The invention also discloses an application method of a diagnostic model of acute myeloid leukemia, in which the serum of a subject is brought into the diagnostic model of acute myeloid leukemia, and whether the subject is ill is judged through calculation. The diagnostic model obtained by the present invention is accurate and efficient, and avoids invasiveness to patients; the application method of the present invention is simple and easy to obtain, has high sensitivity, can non-invasively, efficiently and accurately distinguish acute myeloid leukemia patients from healthy persons, and is suitable for popularization use.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for establishing a diagnosis model of acute myeloid leukemia and its application. Background technique [0002] Acute myeloid leukemia (also known as acute myeloid leukemia or acute myeloid leukemia) is a hematological malignancy characterized by abnormal proliferation and differentiation of bone marrow cells, and it is also the main pathological type of adult acute leukemia (accounting for about 80% -90%), the clinical manifestations are complicated, most of the cases are acute and severe, and the prognosis is dangerous, if not treated in time, it can often be life-threatening. At present, the diagnosis of acute myeloid leukemia mainly relies on peripheral blood cell examination and bone marrow biopsy, both of which have certain limitations. A major limitation of peripheral blood cell testing is that many patients do not have detectable circulating cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 陈鹏谭光国张雅吴剑维张峰王超丽雷杰赵广义王雪娇
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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