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Method for extracting flavonoid pigments from water chestnut peels

A technology for water chestnut skin and flavonoids, which is applied in the field of extracting pigments from water chestnut skin, can solve the problems of low biological activity, low extraction rate, long time consumption and the like, and achieves the effects of simple operation, high flavonoid yield and short time consumption.

Inactive Publication Date: 2019-04-19
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore the purpose of the present invention is to improve the problems of low extraction rate, high energy consumption, poor effect, long time consumption and low biological activity in the extraction process of water chestnut skin pigment, and to provide a high extraction rate, mild and easy method from water chestnut skin. Method for extracting natural pigments from

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment provides a method for extracting flavonoid pigments from water chestnut skin, which includes the following steps: (1) crushing, cleaning, and grinding water chestnut skin; (2) performing enzymolysis and centrifugation after grinding to obtain a precipitate; wherein, the The enzymatic hydrolysis method mentioned is to digest with proteinase K and DTT. The digestion is carried out by using 1mg / ml proteinase K solution and 100mg / ml DTT solution, the digestion time is 7min, the water chestnut skin tissue after grinding is dissolved in PBS solution, DTT solution is added, after shaking well, protease is added K solution and DTT solution were used for digestion reaction, and the method of enzymatic hydrolysis was to digest twice with proteinase K and DTT.

[0027] Add the ground tissue solution to the PBS solution according to the mass volume ratio of 1:10g / μl, and then add the DTT solution. K solution and DTT solution, the addition amount of proteinase K sol...

Embodiment 2

[0030] This embodiment provides a method for extracting flavonoid pigments from water chestnut skin, which includes the following steps: (1) crushing, cleaning, and grinding water chestnut skin; (2) performing enzymolysis and centrifugation after grinding to obtain a precipitate; wherein, the The enzymatic hydrolysis method described is to digest with proteinase K and DTT, and digestion is to utilize the proteinase K solution of 2mg / ml and the DTT solution of 150mg / ml to digest, and the time of described digestion is 10min; In PBS solution, add DTT solution, shake well, add proteinase K solution and DTT solution to carry out digestion reaction; the method of enzymatic hydrolysis is to digest twice with proteinase K and DTT.

[0031] Add the ground tissue solution to the PBS solution according to the mass volume ratio of 1:20g / μl, and then add the DTT solution. K solution and DTT solution, the addition amount of proteinase K solution is 20:1g / μl according to the mass volume rat...

Embodiment 3

[0034]In this embodiment, a method for extracting flavonoid pigments from water chestnut skins comprises the following steps: (1) pulverizing, cleaning, and grinding water chestnut skins; (2) performing enzymolysis and centrifugation after grinding to obtain sediment; wherein, the The enzymatic hydrolysis method is to digest with proteinase K and DTT, and the digestion is to utilize the proteinase K solution of 5mg / ml and the DTT solution of 200mg / ml to digest, and the time of described digestion is 12min, the water chestnut skin tissue after grinding Dissolve in PBS solution, add DTT solution, shake well, add proteinase K solution and DTT solution to carry out digestion reaction, the method of enzymatic hydrolysis is to digest twice with proteinase K and DTT.

[0035] The method of enzymatic hydrolysis is: add the ground tissue solution to the PBS solution according to the mass volume ratio of 1:30g / μl, and then add the DTT solution. Add proteinase K solution and DTT solution...

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Abstract

The invention discloses a method for extracting flavonoid pigments from water chestnut peels, which comprises the following steps: (1) crushing, cleaning and grinding the water chestnut peels; (2) carrying out enzymolysis after grinding, and centrifuging to obtain a precipitate; wherein the enzymolysis method comprises the step of digesting with protease K and DTT. The method for extracting flavonoid pigments from water chestnut peels utilizes protease K and DTT to degrade protein, through Triton X-100 solution, through PBS solution and double distilled water to wash repeatedly, and releases the flavonoid pigments. The method for extracting flavonoid pigments from water chestnut peels has the advantages that the flavonoid yield is high, and the original biological activity can still be kept to the maximum extent; the extracted flavonoid pigment has good stability and high pigment quality. The method has the characteristics of mildness, high efficiency, simplicity, convenience, low energy consumption and the like, is applied to the field of natural pigment extraction, has greater advantages, and has wide development prospect.

Description

technical field [0001] The invention relates to the technical field of natural pigment extraction, in particular to a method for extracting pigment from water chestnut skin. Background technique [0002] China's horseshoe production accounts for more than 95% of the world's total. my country's annual total processing capacity of water chestnuts exceeds 1 million tons, and water chestnut peels account for about 20% of the total water chestnut quality. The water chestnut skin waste produced every year is more than 200,000 tons, and the resources are very large. Water chestnut skin is rich in natural flavonoid pigments, starch, cellulose, polyphenols, polysaccharides and other ingredients. But water chestnut skin has always been used as feed, or directly discarded, which has caused a waste of resources. Therefore, it is of great significance to extract natural pigments from water chestnut skin and develop and utilize waste resources. As a food additive, natural pigments have ...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
Inventor 常超李明董志文雷媛媛邢琛张挽挽李漫李评
Owner WUHAN POLYTECHNIC UNIVERSITY
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