Extraction technology of podocarpus macrophyllus fruit flavones, optimized by response surface method

A technology of response surface optimization and Podocarpus, applied in the direction of medical preparations containing active ingredients, anti-toxins, and medical raw materials derived from gymnosperms, can solve the problems of few reports on the extraction of Podocarpus flavonoids, and achieve drug saving Environment, simple extraction process, high yield of flavonoids

Inactive Publication Date: 2019-07-16
JIAXING VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are few reports about the extraction of Podocarpus flavonoids

Method used

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  • Extraction technology of podocarpus macrophyllus fruit flavones, optimized by response surface method
  • Extraction technology of podocarpus macrophyllus fruit flavones, optimized by response surface method
  • Extraction technology of podocarpus macrophyllus fruit flavones, optimized by response surface method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Response Surface Optimization Spectral Scanning Assisted Extraction of Podocarpus Flavonoids

[0061] 1. Extraction of Podocarpus flavonoids

[0062] Take Podocarpus fruit powder 2.500g, use different solvents according to a certain ratio of solid to liquid, extract for a certain period of time at an ultrasonic frequency of 40KHz, an ultrasonic power of 200W and a certain extraction temperature, and centrifuge (3000r / min, 10min) to obtain The supernatant was stored in a refrigerator at 4°C for later use. The optimal ratio was determined by single factor test and response surface method.

[0063] 2. Determination of antioxidant activity

[0064] ① Determination of DPPH clearance rate

[0065] The antioxidant activity of the samples was measured by the DPPH free radical scavenging method. Determination of DPPH free radical scavenging of Podocarpus pine fruit extract with reference to Qin Manman et al. Pharmacology, 2014, 25(2):185-188), with modifications....

Embodiment 2

[0114] A response surface optimization spectral scanning assisted extraction process of podocarpus flavonoids, the process steps are:

[0115] (1) Raw material pretreatment: wash the Podocarpus fruit, fix it for 5-6 minutes, dry it at 55-60°C, and pulverize it to obtain the Podocarpus fruit powder;

[0116] (2) Extraction of flavonoids: add ultrapure water to Podocarpus powder, the ratio of solid to liquid is 1:30 (g / mL), the ultrasonic temperature is 69.2°C, the ultrasonic time is 2.97h, the ultrasonic frequency is 40KHz, and the ultrasonic power is 200W. The flavonoid concentration (by absorbance) was 3.661A.

Embodiment 3

[0117] Example 3: Response Surface Surface Optimization Ultrasonic Assisted Extraction of Podocarpus flavonoids

[0118] 1 Test method

[0119] 1.1 Extraction of antioxidant components

[0120] Take by weighing 2.500g of Podocarpus pine fruit sample, use different solvents to press certain solid-liquid ratio, in ultrasonic frequency 40KHz, under ultrasonic power 200W and certain extraction temperature, leaching certain hour, after centrifugation, the gained solution is diluted to constant volume to A certain volume, shake well to obtain the sample solution.

[0121] 1.2 Response surface experimental design

[0122] On the basis of the single factor experiment, three factors that have a significant impact on the antioxidant activity were selected as the investigation objects, that is, the amount of enzyme added (A), the time of enzymatic hydrolysis (B), and the temperature of enzymatic hydrolysis (C) as independent variables. Concentration (Y 1 ) and clearance of DPPH (Y 2...

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Abstract

The invention relates to a plant flavone extraction technology, in particular to an extraction technology of podocarpus macrophyllus fruit flavone, optimized by a response surface method, and belongsto the technical field of food science and engineering. Based on a single-factor experiment, Box-Behnken response surface experiment design is adopted, analysis is performed according to Design-Expert8.0.6 software, optimization of the response surface method is performed on the extraction technology of the podocarpus macrophyllus fruit flavone, and a regression model of the extracted podocarpusmacrophyllus fruit flavone is established, so that the optimal ultrasonic extraction technology of the podocarpus macrophyllus fruit flavone is determined. The method has the advantage of being high in flavone yield, the extraction technology is succinct, the extraction condition is mild, and the method is more precise, time-saving, lower in the quantity of consumed medicines and environmentally-friendly than a colorimetric method.

Description

technical field [0001] The invention relates to an extraction process of plant flavonoids, in particular to an extraction process of podocarpus flavonoids optimized by response surface method, and belongs to the technical field of food science and engineering. Background technique [0002] Podocarpus, also known as Podocarpus, soil fir, scientific name Podocarpus macrophyllus (Thumb.) D.Don, a genus of gymnosperm Podocarpus family, is an evergreen tree. In recent years, scholars at home and abroad have conducted many studies on the chemical components and pharmacological effects of Podocarpus medicinal materials. The biflavone components contained in Podocarpus plants have anti-tumor, anti-virus, anti-oxidation, anti-inflammatory and effects on the blood system and nervous system. and so on showed better activity. Podocarpus seed extract has obvious inhibitory effect on human gastric cancer cell line MGC-803 in vitro. The alcoholic extract of Podocarpus fruit has the funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/15A61P39/06A61K131/00
CPCA61K36/15A61K2236/15A61K2236/331A61P39/06
Inventor 沈杰高诗佳张瑞
Owner JIAXING VOCATIONAL TECHN COLLEGE
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