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An automated genotyping device and method

A genotyping and equipment technology, applied in biochemical equipment and methods, biochemical instruments, biochemical cleaning devices, etc., can solve the problems of inability to do ultra-micro-pipetting, low precision and stability, and affecting experimental results, etc. , to achieve the effect of rapid change and precise control, fewer experimental operators, and improved amplification efficiency

Active Publication Date: 2022-06-28
成都瀚辰光翼科技有限责任公司
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AI Technical Summary

Problems solved by technology

However, the existing genotyping system has the disadvantages of simple device structure, single function, low precision and stability, high single detection cost, low throughput or low degree of automation, and it is impossible to achieve ultra-micro transfer in the working process. During the pipetting process, it may come into contact with the reagents to cause pollution and affect the final experimental results.

Method used

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  • An automated genotyping device and method
  • An automated genotyping device and method

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Embodiment Construction

[0038] The present invention will be further described below in conjunction with the accompanying drawings:

[0039] like figure 2 , image 3 , Figure 4 As shown, an automated genotyping equipment includes a sample transfer device for moving reagents or DNA samples, a water bath device for amplifying PCR, a data acquisition device for collecting various data, a sample transfer PLC module, and a water bath PLC module. , Acquisition PLC module and data analysis module;

[0040] The sample pipetting device includes a non-contact pipetting device 1 for moving reagents, a contact pipetting device 14 for moving DNA samples, a reagent tank containing reagents, a well plate containing DNA samples, and a heat-sealing device. The contact pipetting device 1 includes a reaction plate, the non-contact pipetting device 1 is used for moving the reagent in the reagent tank or the DNA sample in the well plate, the heat sealing device is used for heat sealing the reaction plate with the reag...

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Abstract

The invention discloses an automatic genotyping device and method, and relates to the field of genotyping. The genotyping device includes a sample transfer device, a water bath device, a data collection device, a sample transfer PLC module, a water bath PLC module, a collection PLC module and data The analysis module; the genotyping method includes: setting the pipetting program; configuring reagents; adding DNA samples to the well plate; placing the reaction plate at the pipetting position; The sealed reaction plate is moved into the water bath box; the data of the reaction plate after the water bath is collected by using the data acquisition device; High usage requirements, the use of this equipment can save a lot of manpower and improve the accuracy of experimental results.

Description

technical field [0001] The invention relates to the field of genotyping, in particular to an automatic genotyping device and method. Background technique [0002] Genotyping technology has been widely used in the identification of animal and plant species, molecular typing of pathogenic bacteria, human identification (forensic identification, paternity testing, etc.) analysis, etc. However, the existing genotyping systems have the disadvantages of simple device structure, single function, low accuracy and stability, high single detection cost, low throughput or low degree of automation, and cannot achieve ultra-micro transfer in the working process. During the pipetting process, it may come into contact with the reagents, causing contamination and affecting the final experimental results. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to design an automatic genotyping device and method in order to solve the above problems. [0004] The present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/36C12M1/34
CPCC12Q1/686C12Q2537/165
Inventor 李杰贾洵李伟嘉向华林陈石
Owner 成都瀚辰光翼科技有限责任公司
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