Automatic genotyping equipment and method

Abstract

The invention discloses an automatic genotyping equipment and method, and relates to the field of genotyping. The genotyping equipment comprises a sample shifting device, a water bath device, a data acquisition device, a sample shifting PLC module, a water bath PLC module, a collection PLC module and a data analysis module; the genotyping method comprises the steps that a liquid shafting program is set; a reagent is prepared; a DNA sample is added into an orifice plate; a reaction plate is placed at the liquid shifting position; a liquid shifting scheme is selected, the liquid shifting programis carried out, and the reaction plate is encapsulated through an automatic heat sealing machine; the reaction plate obtained after heat sealing is moved into a water bath box; data of the reaction plate after the water bath is collected by means of the data acquisition device; the result of sample classification is read by means of the data analysis module. Accordingly, the use requirements thatthe sample volume is larger, fewer experimental operators are needed, and the requirement is high can be achieved; a lot of manpower can be saved, and the accuracy of the experimental results is improved.

Description

technical field [0001] The invention relates to the field of genotyping, in particular to an automatic genotyping device and method. Background technique [0002] Genotyping technology has been widely used in the identification of animal and plant species, molecular typing of pathogenic bacteria, human identification (forensic identification, paternity identification, etc.), disease-related genotypes due to its excellent biological recognition resolution and near-trace detection sensitivity. analysis etc. However, the existing genotyping system has the disadvantages of simple device structure, single function, low precision and stability, high single detection cost, low throughput or low degree of automation, and cannot achieve ultra-micro transfer in the working process. The liquid may come into contact with the reagent during the pipetting process, causing contamination and affecting the final experimental results. Contents of the invention [0003] The object of the p...

AI Technical Summary

Problems solved by technology

However, the existing genotyping system has the disadvantages of simple device structure, single function, low precision and stability, high single detection cost, low throughput or low degree of automation, and it is impossible to achieve ultra-micro transfer in the working process. During the pipetting process, it may come into contact with the reagents to cause pollution and affect the final experimental results.

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