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Application of zinc-adsorbing bacterial strain and cultivation method of zinc-adsorbing bacterial strain

A culture method and strain technology, applied in the field of microorganisms, can solve the problems of large investment, difficulty in meeting water standards, and high operating costs, and achieve the effects of broad application prospects, short time-consuming, and convenient operation

Active Publication Date: 2019-04-19
北京本农科技发展有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with traditional physical methods, these methods have problems such as large investment, high operating costs, difficulty in reaching the standard after treatment, and large sludge production.
The microbial method requires less investment and no secondary pollution. However, the microbial method in the prior art generally has low treatment efficiency and low adsorption rate of zinc ions, which has limited its application and cannot expand the application range of the biological method market.

Method used

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  • Application of zinc-adsorbing bacterial strain and cultivation method of zinc-adsorbing bacterial strain
  • Application of zinc-adsorbing bacterial strain and cultivation method of zinc-adsorbing bacterial strain
  • Application of zinc-adsorbing bacterial strain and cultivation method of zinc-adsorbing bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The culture method of zinc adsorption bacterial strain specifically comprises the following steps:

[0064] 1) Preparation of zinc adsorption mother liquor: ZnSO 4 ·7H 2 Stir and mix O with water evenly, the concentration is 7g / L;

[0065] 2) Nutrient broth medium: peptone 12g / L, beef powder 1g / L, sodium chloride 6g / L, glucose 0.5g / L, solvent is water, prepare medium for zinc adsorption and enrichment, and absorb zinc according to the proportion The mother liquor is added to the nutrient broth medium, the concentration of zinc ions is 70mg / L, and it is sterilized at 110°C for 10 minutes for later use. Concentration of 60mg / L, sterilized at 120°C for 20min for later use;

[0066] 3) The black-stinky bottom mud sample (the nutrient broth culture medium obtained from the primary screening of the sea-smelly bottom mud) was subjected to tolerance and acclimatization in the above-mentioned enrichment medium, and each generation of acclimatization took 7 days, and a total o...

Embodiment 2

[0070] The culture method of zinc adsorption bacterial strain specifically comprises the following steps:

[0071] 1) Preparation of zinc adsorption mother liquor: ZnSO 4 ·7H 2 Stir and mix O with water evenly, the concentration is 3g / L;

[0072] 2) Nutrient broth medium: peptone 8g / L, beef powder 4g / L, sodium chloride 3g / L, glucose 3g / L, solvent is water, prepare medium for zinc adsorption and enrichment, and zinc adsorption mother liquor according to the ratio Add it to the nutrient broth medium, the concentration of zinc ions is 65mg / L, and sterilize it at 115°C for 15 minutes for later use. Concentration: 50mg / L, sterilized at 115°C for 15min and ready for use;

[0073] 3) The black-stinky bottom mud sample (the nutrient broth culture medium obtained from the primary screening of the sea-smelly bottom mud) was subjected to tolerance and acclimatization in the above-mentioned high-concentration enrichment medium, and each generation of acclimatization took 7 days. The m...

Embodiment 3

[0077] The culture method of zinc adsorption bacterial strain specifically comprises the following steps:

[0078] 1) Preparation of zinc adsorption mother liquor: ZnSO 4 ·7H 2 Stir and mix O with water evenly, the concentration is 5g / L;

[0079] 2) Nutrient broth medium: peptone 10g / L, beef powder 3g / L, sodium chloride 5g / L, glucose 1g / L, solvent is water, prepare medium for zinc adsorption and enrichment, and zinc adsorption mother liquor in proportion Add it to the nutrient broth medium, the concentration of zinc ions is 60mg / L, and sterilize it at 115°C for 15 minutes for later use. Concentration: 50mg / L, sterilized at 115°C for 15min and ready for use;

[0080] 3) The black-stinky bottom mud sample (the nutrient broth culture medium obtained from the primary screening of the sea-smelly bottom mud) was subjected to tolerance and acclimatization in the above-mentioned high-concentration enrichment medium, and each generation of acclimatization took 7 days. The mixed bac...

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Abstract

The invention relates to the field of microorganisms, in particular to specific application of a zinc-adsorbing bacterial strain. The bacterial strain is preserved in China General Microbiological Culture Collection Center, the preservation number is CGMCC No:15930, and the preservation time is June 11st, 2018. The bacterial strain has an excellent effect of adsorbing zinc ions, after 48 h, the zinc ion adsorbing and degrading rate can maximally reach 97.78%, and therefore, the bacterial strain has a huge potential in the aspect of water contamination treatment on heavy metal zinc ions, and has a very broad application prospect.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of a zinc-adsorbing strain and a method for cultivating the zinc-adsorbing strain. Background technique [0002] Zinc is a heavy metal resource that is abundant in the earth. my country's zinc ore resource reserves rank second in the world, and zinc resources are widely used in modern industrial production such as smelting, pharmaceutical and food industries. Zinc is an indispensable element for the human body. It exists widely in human muscles and bones, but the content is very small. If it is excessive, serious consequences will occur. The discharge of zinc-containing wastewater is a serious hazard to human health and industrial and agricultural activities. It is persistent, highly toxic, and seriously polluting. Once it enters the environment, it cannot be biodegraded. Most of it participates in the food chain cycle and eventually accumulates in organisms. Destr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/20
CPCC02F3/34C02F2101/20C12N1/20C12N1/205C12R2001/38
Inventor 王玉文韩建均赵川梁雪杰
Owner 北京本农科技发展有限公司
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