Detection method of 2-methyl isoborneol in aquatic products

A technology for methyl isocyanide and aquatic products, which is applied in the field of detection of 2-methylisocyanidol, a fishy substance in aquatic products, can solve the problems of high operator requirements, expensive instruments, large influence of subjective factors, etc. It is suitable for promotion, improving accuracy, and the effect of low detection limit

Active Publication Date: 2019-04-19
江西中德生物工程股份有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The sensory analysis method has the advantages of low cost and high efficiency, but the subjective factors of this method are too influential to carry out accurate quantitative analysis
The detection of odorous substances by instrumental analysis is mainly separated by gas chromatography and then detected by hydrogen flame ionization detector or mass spectrometer. The advantage of instrumental analysis is that the sensitivity and accuracy are very high. It is very critical and time-consuming, and the instruments used are very expensive and require a lot of operators

Method used

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  • Detection method of 2-methyl isoborneol in aquatic products
  • Detection method of 2-methyl isoborneol in aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Sample treatment: take 5 g of fish meat from the gills of shad fish, mash it, and extract it ultrasonically in 20 mL of methanol to obtain an extract. The ultrasonic power is 200W, the ultrasonic frequency is 20kHz, and the ultrasonic time is 2min.

[0039] (2) Preparation of nano-gold solution: get the leaves of Lycoris safflower, put into pulverizer and pulverize to obtain powder; Get 2g of Lycoris safflower leaf powder, drop into 100mL of chloroauric acid solution, and mix evenly at room temperature Reaction; react for a period of time to obtain gold nanoparticle aqueous solution, centrifuge and dry to obtain nanogold; transfer quantitative gold nanoparticles into a centrifuge tube, add methanol at the same time, cover the tube cap and place it in an ultrasonic machine, and obtain 1mg / mL of nano-gold methanol solution.

[0040] (3) Electrochemical detection: Use a pipette gun to drop 1 μL nano-gold methanol solution on the surface of the glassy carbon electrode...

Embodiment 2

[0044] (1) Sample processing: Take 10 g of meat from the gills of Eriocheir sinensis, mash it, and extract it ultrasonically in 10 mL of methanol to obtain an extract. The ultrasonic power is 200W, the ultrasonic frequency is 20kHz, and the ultrasonic time is 2min.

[0045](2) Preparation of nano-gold solution: get the leaves of Lycoris safflower, put into pulverizer and pulverize to obtain powder; Get 2g of Lycoris safflower leaf powder, drop into 100mL of chloroauric acid solution, and mix evenly at room temperature Reaction; react for a period of time to obtain gold nanoparticle aqueous solution, centrifuge and dry to obtain nanogold; transfer quantitative gold nanoparticles into a centrifuge tube, add methanol at the same time, cover the tube cap and place it in an ultrasonic machine, and obtain 1mg / mL of nano-gold methanol solution.

[0046] (3) Electrochemical detection: Use a pipette gun to drop 1 μL nano-gold methanol solution on the surface of the glassy carbon elec...

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Abstract

The invention discloses a detection method of 2-methyl isoborneol in aquatic products. The detection method includes: utilizing lycoris radiata to reduce a precursor-chloroauric acid to obtain nanogold particles, using the nanogold particles to modify a glassy carbon electrode, utilizing and observing electrochemical change of a to-be-detected sample on the glassy carbon electrode after being modified, comparing with a linear curve to obtain 2-methyl isoborneol concentration a, using a Ze-ta potentiometric analysis method to measure 2- methyl isoborneol concentration b, and taking an arithmetic average value of a and b as concentration of the to-be-detected sample. Instruments used in the detection method are economical, practical, suitable for popularization, little in sample demand and low in detection limit, and accuracy of objective evaluation is improved.

Description

technical field [0001] The invention relates to the technical field of food quality detection, in particular to a method for detecting fishy-causing substance 2-methylisobutyritol in aquatic products. Background technique [0002] Aquatic products are rich in various vitamins and minerals, and are a good source of various macro and trace elements required for a healthy diet. With the deepening of people's understanding of the nutritional value of aquatic products, the market and consumer groups of aquatic products are gradually expanding , the demand is increasing year by year. However, no matter whether it is naturally caught fish or artificially farmed aquatic products, there is an earthy smell to varying degrees. This earthy smell not only reduces the edible value and breeding benefits of aquatic products, but also has food safety problems. It affects the quality and taste of aquatic products, discourages some consumers, and has a certain impact on the sales and breeding...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/48
CPCG01N27/416G01N27/48
Inventor 傅力刘青华吴梦瑶
Owner 江西中德生物工程股份有限公司
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