Liquid culture medium suitable for tender stems of dendrobium officinale and preparation method of liquid culture medium

A technology of liquid culture medium and Dendrobium candidum, which is applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of unsatisfactory polysaccharide and extract content, etc.

Inactive Publication Date: 2019-04-23
苏州普朗特尔皮肤细胞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, first of all, these mediums are aimed at the growth of Dendrobium candidum, which belong to the soil culture medium, and there is no liquid medium specifically for the growth of the tender stems of Dendrobium candidum; secondly, organic additives are added to the cultivation of Dendrobium candidum relatively seldom Moreover, the content of polysaccharides and extracts in the current tender stems of Dendrobium officinale is not ideal

Method used

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  • Liquid culture medium suitable for tender stems of dendrobium officinale and preparation method of liquid culture medium

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preparation example Construction

[0093] The present invention also discloses a method for preparing a liquid culture medium suitable for the tender stems of Dendrobium officinale, comprising the following steps:

[0094] S1, add coconut milk, apple extract, potato extract, major elements including potassium, nitrogen, phosphorus, magnesium, trace elements including iodine, boron, molybdenum, iron salts and organic matter at room temperature for 5 - Mix with 7 times the amount of water to get product A;

[0095] S2, predissolve 2, 4-D and NAA in absolute ethanol to prepare a solution of 0.5-1.5 mg / ml to obtain product B;

[0096] S3, mix products A and B in tissue culture bottles, sterilize under high-temperature steam for 20-30 minutes, and cool to obtain.

[0097] Concrete formula embodiment is as follows:

Embodiment 1

[0099] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, potato extract Extract 10%, Apple Extract 5%, Coconut Juice 5%, 2,4-D 2mg / L, NAA 0.5mg / L.

Embodiment 2

[0101] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, potato extract Extract 5%, Apple Extract 5%, Coconut Juice 5%, 2,4-D 2mg / L, NAA 0.5mg / L.

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Abstract

The invention discloses a preparation method of a liquid culture medium suitable for tender stems of dendrobium officinale. The preparation method comprises the following steps of adding coconut juice, a potato extract, an apple extract and other materials into 5 to 7 times of water under room temperature, and uniformly mixing to obtain a product A; pre-dissolving 2,4-D and NAA (naphthylacetic acid) by anhydrous ethanol, and preparing into a solution with concentration of 0.5 to 1.5 mg/ml to obtain a product B; and loading the product A and the product B into a tissue culture bottle, uniformlymixing, sterilizing for 20 to 30 min by steam at high temperature, and cooling to obtain the liquid culture medium. The liquid culture medium disclosed by the invention has the beneficial effects that by adding and controlling the concentrations of the coconut juice, the apple extract and the potato extract, the contents of polysaccharide and extracts in the tender stems of the dendrobium officinale are increased.

Description

technical field [0001] The invention relates to the field of agricultural planting, in particular to a liquid culture medium suitable for the tender stems of Dendrobium officinale and a preparation method thereof. Background technique [0002] Dendrobium officinale (Dendrobium officinale) is a perennial herbaceous Chinese herbal plant belonging to Magnoliophyta, Liliopsida, Asparagales, Orchidaceae, and Dendrobium. It is recorded in the Chinese Pharmacopoeia (2015 edition) that Dendrobium candidum has the effects of clearing heat and purging fire, detoxifying, diuresis and swelling, treating fever and polydipsia, sore throat, edema, oliguria, hot stranguria and pain, and carbuncle. Similarly, modern medical research shows that Dendrobium candidum has the function of enhancing immune T cells, which can improve human immunity, remove aging and mutated cells, stabilize blood sugar, increase cell metabolism rate, and maintain the physiological stability of the body. [0003] Wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张杰王昕怡吴红升
Owner 苏州普朗特尔皮肤细胞科技有限公司
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