Agonist peptide for adiponectin receptor

一种序列、氨基酸的技术，应用在激素肽、肽、特定肽等方向，能够解决损伤或破坏、注射的不便或反感大等问题

Pending Publication Date: 2019-04-26
B B 金
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, in the case of existing oral diabetes therapeutic agents used clinically, in addition to the positive effect of maintaining normalization of blood sugar, when taken for a long time, not only induce hypoglycemia, diarrhea, abdominal distension, weight gain, blood Various side effects such as lactic acid, cardiotoxicity, and hepatotoxicity, and finally, the β cells of the pancreas, which function as insulin secretion, are irreversibly damaged or destroyed, resulting in insulin resistance, so the drug is ineffective and insulin injections are required
In addition, in the case of insulin, which is most used as a therapeutic agent for diabetes, it needs to be injected subcutaneously 2 to 3 times a day, so it is very inconvenient or disgusting to inject, and there is also a problem that the possibility of inducing hypoglycemia is very high.

Method used

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  • Agonist peptide for adiponectin receptor
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  • Agonist peptide for adiponectin receptor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Synthesis and separation and purification of ADIPOR1 recombinant protein

[0034] ADIPOR1 protein sequence Using the same N-terminal (N-terminal) excluding 88 amino acid sequence used in ADIPOR1 structural analysis, a gene (IDT) that can produce the protein sequence was synthesized through codon optimization (codon optimization) process. At this point, for good cloning and purification of expressed proteins, a BamHI restriction enzyme site, a flag tag (Flag tag) and a TEV protease recognition sequence (ENLYFQG) were inserted at the 5' end (end), 3 ' end into an EcoRI restriction enzyme site. The obtained gene was put into the pFASTbacl vector (pFASTbacl vector) required for the production of baculovirus (baculovirus) to perform cloning. The cloned vector was injected into DH10bac (Invitrogen) and recombined to obtain a bacmid containing the ADIPOR1 gene. In order to make baculovirus, the bacmid obtained by transfection (Transfectine) (Qiagen) was used to tr...

Embodiment 2

[0035] Example 2: Preserving cells

[0036] C2C12 and HepG2 cells were purchased from ATCC, 10% fetal bovine serum (FBS) (Hyclone) and 1% penicillin / streptomycin were added to DMEM (Hyclone) medium, and the cells were incubated at 37°C and 5% CO 2 The environment is cultivated in an incubator for use.

Embodiment 3

[0037] Example 3: C2C12 Myotube Differentiation

[0038] After C2C12 cells were seeded in 12 wells (well), when there was more than 80% confluency, they were washed once with phosphate buffered saline (PBS), and then 2.5% horse serum (Horse serum) and 1 % penicillin / streptomycin differentiation medium, after that, replace the medium with a new differentiation medium every day, and use it in the experiment after 5-7 days of differentiation.

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Abstract

An agonist peptide for an adiponectin receptor is provided according to an embodiment of the present invention, which allows increased affinity between an agonist peptide for an adiponectin receptor and the adiponectin receptor, thereby effectively activating AMPK phosphorylation. Also, a therapeutic agent is provided according to an embodiment of the present invention, which minimizes a side effect of a therapeutic agent for type 2 diabetes and simultaneously exhibits greater efficacy than the conventional therapeutic agents.

Description

technical field [0001] The present invention relates generally to the fields of pharmacy, cell biology and molecular biology, and more particularly to agonist peptides acting on the adiponectin receptor for the treatment of type 2 diabetes. Background technique [0002] Diabetes is a serious metabolic disease with symptoms of hyperglycemia, caused by insulin secretion problems, such as decreased insulin secretion and resistance due to genetic and environmental reasons, or abnormal insulin function, and the glucose in the blood cannot Transferred or stored in the cells and excessively increased in the blood, making the value of blood sugar much higher than normal. Diabetes is broadly classified into type 1 diabetes (Type 1 Diabetes: T1), type 2 diabetes (Type 2 Diabetes), and gestational diabetes (Gestational Diabetes). [0003] In particular, type 2 diabetes related to the present invention accounts for the majority of diabetic patients in Korea. Unlike type 1 diabetes, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10C12N15/11
CPCC07K7/08A61K38/10A61K47/60A61K47/64A61P3/10C07K14/5759
Inventor B·B·金
Owner B B 金
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