Sodium alginate-dopamine/polyvinyl alcohol hydrogel, and preparation method and application thereof

A sodium alginate and polyvinyl alcohol technology, applied in medical science, bandages, etc., can solve the problems of poor wound healing, complicated preparation process, and high reaction conditions, and achieve broad biomedical application prospects, improve mechanical properties, and absorb water. The effect of sexual comfort

Active Publication Date: 2019-05-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing hydrocolloid dressings only consider biocompatibility and mechanical properties when designing, and do not introduce a substance that can promote wound healing. This type of dressing has obvious disadvantages: such as high preparation cost, complicated preparation process and Difficult to remove impurities, high reaction conditions and poor wound healing effects, etc., cannot comprehensively solve the post-care problems of pressure sores

Method used

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  • Sodium alginate-dopamine/polyvinyl alcohol hydrogel, and preparation method and application thereof
  • Sodium alginate-dopamine/polyvinyl alcohol hydrogel, and preparation method and application thereof
  • Sodium alginate-dopamine/polyvinyl alcohol hydrogel, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Sterilization of experimental raw materials and instruments

[0041] The experimental raw materials were sterilized by irradiating the raw materials with ultra-clean bench for 1 hour, and the glass instruments were sterilized at 121°C for 20 minutes under high-pressure conditions.

[0042] (2) Preparation of sodium alginate-dopamine

[0043] Take 2g of sodium alginate (SA) and add it into 100ml of distilled water, stir and dissolve at 800rpm for 12h, and prepare an SA solution for later use. Add 0.5755g N-hydroxysuccinimide (NHS) and 0.9585g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the SA solution according to the molar ratio of 1:1 and add Stir at 800 rpm for 20 min, then add 1.5473 g of dopamine (DA) at room temperature and stir at 800 rpm for 24 hours. After the reaction is completed, the prepared sodium alginate-dopamine (SA-DA) solution is sealed and stored at 2-8°C.

[0044] (3) Preparation of sodium alginate-dopamine / polyvinyl alcohol...

Embodiment 2

[0060] In Vitro Cell Experiment of Sodium Alginate-Dopamine / Polyvinyl Alcohol Hydrogel

[0061] Before the in vitro cytotoxicity test, the freeze-dried hydrogel sample prepared in Example 1 was sterilized by Co60 γ-ray radiation.

[0062] (1) Take out NIH 3T3 cells (purchased from Wuhan Punuosai Life Technology Co., Ltd.) in liquid nitrogen and resuscitate them with DMEM culture medium.

[0063] (2) Subculture several times with DMEM culture medium, take the cells in the logarithmic growth phase and count the NIH3T3 cells with a cell counting plate, adjust the cell density to 5×10 4 individual / mL.

[0064] (3) Take a 96-well plate and add 100 μL to 30 wells of it. Step (2) The cell density is 5×10 4 cells / mL of DMEM culture solution was used as the experimental group; at the same time, another 3 wells were taken and set as a blank control group, that is, only 100 μL of DMEM culture solution (without cells) was added, and placed at 37°C, 5% CO 2 Incubate for 24 hours in a ce...

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Abstract

The invention discloses a sodium alginate-dopamine/polyvinyl alcohol hydrogel, and a preparation method and application thereof. The preparation method comprises the following steps: grafting dopamine(DA) on sodium alginate (SA) through chemical cross-linking to introduce nerve conduction substances, communicate cells and convey chemical substances, so that a wound healing effect is promoted, physical adhesion capability is improved, and biocompatibility of the material is improved; then combination is carried out with medicine-grade polyvinyl alcohol (PVA) which has good biocompatibility, ischeap and is easy to obtain, so that physical performance of hydrogel is improved, and therefore, the hydrogel with relatively high mechanical strength is prepared. The hydrogel provided by the invention has good biocompatibility and excellent physical performance, the reaction raw materials are cheap and are easy to obtain, reaction conditions are mild, and operation steps are simple and convenient. The hydrogel provided by the invention has a wide application prospect in bedsores wound care and biomedicine.

Description

technical field [0001] The invention belongs to the field of biopolymer materials, and in particular relates to a sodium alginate-dopamine / polyvinyl alcohol hydrogel and a preparation method and application thereof. Background technique [0002] Bed sores, also known as pressure sores, are a common clinical complication. The main external causes of pressure sores are the pressure factors: vertical pressure (local tissue is subject to continuous vertical pressure, such as body bone tuberosity), friction (skin friction), shear force (a force applied to an object After getting up, it will lead to a plane sliding in parallel and opposite direction. For example, when the body slides down when the head of the bed is raised, the parallel friction between the skin and the bed, coupled with the gravity in the vertical direction of the skin, will lead to the generation of shear force and cause local skin blood. circulatory disturbances leading to pressure sores). At present, clinica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L29/04C08L5/04C08B37/04A61L26/00
Inventor 敖宁建赖学旭
Owner JINAN UNIVERSITY
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