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Cover slip preservation solution and preparation method thereof

A technology of preservation solution and coverslip, applied in the preparation of test samples, etc., can solve the problem of mold growth on the surface, and achieve the effect of solving mildew, strong practicability and obvious effect.

Inactive Publication Date: 2019-05-03
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a glass cover slip preservation solution and its preparation method in view of the defects that the existing conventional glass cover glass preservation method is very easy to cause surface mold growth and the like. The preservation solution has strong bactericidal ability, fast action and good effect At the same time, the preservation solution has stable properties, no failure, and no special irritating odor. It is an ideal preservation solution for glass coverslips.

Method used

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  • Cover slip preservation solution and preparation method thereof
  • Cover slip preservation solution and preparation method thereof

Examples

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Embodiment 1

[0025] The cover slip preservation solution comprises absolute ethanol, potassium dichromate, concentrated nitric acid, concentrated hydrochloric acid, Tris saturated phenol; wherein the mass percent concentration of potassium dichromate in the cover slip preservation solution is 0.25‰, concentrated nitric acid, The volume percent concentration of concentrated hydrochloric acid in the coverslip preservation solution is 0.5%, the volume percent concentration of Tris saturated phenol solution in the coverslip preservation solution is 1.5%, and the balance is absolute ethanol.

[0026] In order to realize the technical purpose of the present invention, taking 500 mL of coverslip preservation solution and its preferred mass / volume fraction as an example, the method for preparing the coverslip preservation solution provided by the present invention comprises the following steps:

[0027] (1) Add 0.125g potassium dichromate to a 500mL beaker;

[0028] (2) Add 450mL of absolute ethan...

Embodiment 2

[0033] Example 2 Experiment

[0034] (1) Take the coverslips of the same specification and place them in the preservation solution one by one to prevent the adhesion of the slides and cause uneven penetration of the preservation solution;

[0035] (2) Tighten the transparent screw-top glass bottle, turn it upside down slowly, and remove some air bubbles between the coverslips;

[0036] (3) Take out the coverslip when necessary (or rinse with distilled water or ultrapure water for 3-5 minutes);

[0037] (4) Dry naturally in a fume hood or dry in an oven at 37°C for 5 minutes before use;

[0038] (5) Or organize them into boxes for long-term storage and use as you go;

[0039] (6) with attached figure 1 For example, the coverslips preserved in the preservative solution are as clean as new, and there is no mold, grease and other attachments with the naked eye; the coverslips preserved in the conventional way are blurred and mildewed;

[0040] (7) to attach figure 2 For exam...

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Abstract

The invention discloses a cover slip preservation solution and a preparation method thereof. The cover slip preservation solution contains the following components of potassium dichromate, concentrated nitric acid, concentrated hydrochloric acid, Tris saturated phenol solution and absolute ethanol, wherein the mass percentage concentration of the potassium dichromate in the cover glass preservation solution is 0.02-0.1%, the volume percentage concentration of the concentrated nitric acid and the concentrated hydrochloric acid in the cover slip preservation solution is 0.5-2%, the volume percentage concentration of the Tris saturated phenol solution in the cover slip preservation solution is 1-10%, and the balance is anhydrous ethanol. The method is advantaged in that the cover slip preservation solution has advantages of simple preparation, economical property, strong antibacterial ability, quick action and good effect, moreover, the preservation solution is stable in nature, is not easy to fail and has no special irritating odor, and the cover glass preservation solution is the ideal glass cover slip preservation solution.

Description

technical field [0001] The invention relates to a cover glass preservation solution and a preparation method thereof. Background technique [0002] Glass coverslips are commonly used consumables in the process of histopathological film production. Two types of coverslips (one is no-cleaning and the other is cleaning before use) are easily affected by moisture during long-term storage, causing The growth of mold on the surface leads to a blurred view of the microscope after the tissue section is mounted, which seriously reduces the clarity of the tissue section and directly affects the diagnosis and analysis results. [0003] At present, the mold and grease on the surface of glass coverslips are usually soaked in chemical cleaning solution. After soaking the long-term preserved coverslips in chemical cleaning solution, they are rinsed with running water, wiped with clean white cotton gauze, and then used after drying. The steps are cumbersome, and the soaking time is mostly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 雷光华何可丁翔刘之晨邓鑫佳陈曼莉曾超李辉
Owner XIANGYA HOSPITAL CENT SOUTH UNIV