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Disease-resistant solanum lycopersicum rootstock root exudate collect fluid and inhibition method thereof on ralstonia solanacearum

A root exudate and collection solution technology, applied in botany equipment and methods, chemicals for biological control, animal repellants, etc., can solve the problems of difficult promotion, complicated operation, small collection volume, etc., and achieve low cost Low cost, high collection efficiency, simple and convenient operation

Inactive Publication Date: 2019-05-07
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current collection methods of root exudates include soil culture method, matrix culture method and nutrient agar culture method, but these methods are complicated to operate, the collection volume is small, and it is difficult to popularize

Method used

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  • Disease-resistant solanum lycopersicum rootstock root exudate collect fluid and inhibition method thereof on ralstonia solanacearum
  • Disease-resistant solanum lycopersicum rootstock root exudate collect fluid and inhibition method thereof on ralstonia solanacearum
  • Disease-resistant solanum lycopersicum rootstock root exudate collect fluid and inhibition method thereof on ralstonia solanacearum

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A method for inhibiting Ralstonia solanacearum by root exudates of disease-resistant tomato stock, the preparation method is as follows:

[0027] (1) Collection of disease-resistant tomato rootstock root exudates:

[0028] 1) Use 50-hole plug trays to grow seedlings of different tomato rootstock varieties. The seedling substrate is prepared according to peat: coco peat: perlite = 2:1:1, and water and fertilizer are added regularly; the tomato rootstock variety is "Fanzhen No. 1" ( No.1), "Qizhen No. 21" (No.21), and the control is Fenbeibei (Fb), a tomato variety highly susceptible to bacterial wilt;

[0029] 2) After sowing, when the three leaves are one heart, transfer to the nutrient bowl for cultivation, the substrate is consistent with the step 1, and the water and fertilizer management is carried out uniformly;

[0030] 3) After the seedlings grow to the budding stage, collect root exudates, take 10 strong seedlings for each variety, carefully take out the seedli...

Embodiment 2

[0047] The effect of root exudates from disease-resistant rootstocks on improving the resistance of tomato to bacterial wilt, the implementation method is as follows:

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Abstract

The invention discloses a disease-resistant solanum lycopersicum rootstock root exudate collect fluid and an inhibition method thereof on ralstonia solanacearum. The inhibition method includes collection of disease-resistant solanum lycopersicum rootstock root exudates and inhibition of ralstonia solanacearum. According to the invention, the collection method of the disease-resistant solanum lycopersicum rootstock root exudate has the characteristics of high collection efficiency, simple operation, safety and environmental protection. The provided test method can visually reflect the inhibitory effect of the disease-resistant solanum lycopersicum rootstock root exudate on pathogens. The root exudate of disease-resistant rootstock can be applied in production to effectively inhibit the biological activity of ralstonia solanacearum in soil, thereby reducing or delaying the morbidity of solanum lycopersicum.

Description

technical field [0001] The invention relates to the technical field of extraction of vegetable metabolites and disease-resistant cultivation, in particular to a liquid for collecting root exudates from disease-resistant tomato rootstocks and a method for inhibiting R. solanacearum. Background technique [0002] Tomato (Solanum lycopersicum Mill.) is one of the fruits and vegetables widely cultivated in my country. Due to unreasonable tillage and water and fertilizer supply, soil continuous cropping obstacles have been aggravated, and soil-borne diseases have occurred seriously in recent years. Ralstonia solanacearum E.F.Smith is a soil-borne vascular disease caused by Ralstonia solanacearum E.F.Smith (hereinafter referred to as R. solanacearum), which often causes large-scale wilting and death of Solanaceae crops. Due to the unique living habits and propagation characteristics of R. solanacearum, it is difficult to control it with conventional chemicals, which poses a great ...

Claims

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Application Information

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IPC IPC(8): A01N65/38A01P1/00A01G31/00
Inventor 于文进赵文宗阳燕娟钟川
Owner GUANGXI UNIV