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Method for simply and rapidly producing PHB/bacterial cellulose composite material

A technology of bacterial cellulose and composite materials, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as single method, achieve simple operation, high tensile strength and elastic modulus, mild conditions Effect

Active Publication Date: 2019-05-10
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the combination of bacterial cellulose and PHB. At present, the method used for combining the two is relatively simple, that is, soaking the purchased bacterial cellulose in a solution of dissolving PHB in chloroform to obtain a composite of PHB / bacterial cellulose Material

Method used

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  • Method for simply and rapidly producing PHB/bacterial cellulose composite material
  • Method for simply and rapidly producing PHB/bacterial cellulose composite material
  • Method for simply and rapidly producing PHB/bacterial cellulose composite material

Examples

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Comparison scheme
Effect test

Embodiment 1

[0026] A kind of method for producing PHB / bacterial cellulose composite material simply and quickly, comprises the steps:

[0027] (1) Microbial culture: Activate the Ralstonia eutropha H16 (DSN428) strain stored in a freeze-drying tube in the seed medium, and after 24 hours of growth, inoculate the obtained bacterial solution into the basal medium Ⅰ with an inoculation amount of 5%. Expand culture to OD 600 When the value is 1, the bacterial solution was inoculated again into the basal medium I with the same inoculum amount for repeated expansion culture to its OD 600 value is 1;

[0028] (2) Activate the Gluconacetobacter xylinus ATCC700178 bacterial strain stored in the freeze-drying tube in basal medium II, and after 48 hours of growth, inoculate the obtained bacterial liquid into basal medium II for expanded culture for 24 hours, the inoculum size is 5%, Take the same inoculated amount of bacterial liquid and inoculate it into the basic medium II again for repeated expans...

Embodiment 2

[0035] A kind of method for producing PHB / bacterial cellulose composite material simply and quickly, comprises the steps:

[0036] (1) Microbial culture: activate the Ralstonia eutropha H16 (DSM428) strain stored in a freeze-drying tube in the seed medium, and after 24 hours of growth, inoculate the obtained bacterial solution into the basal medium Ⅰ with an inoculation amount of 5%. Expand culture to OD 600 When the value is 1, the bacterial solution was inoculated again into the basal medium I with the same inoculum amount for repeated expansion culture to its OD 600 value is 1;

[0037] (2) Activate the Gluconacetobacter xylinus ATCC700178 bacterial strain stored in the freeze-drying tube in basal medium II, and after 48 hours of growth, inoculate the obtained bacterial liquid into basal medium II for expanded culture for 24 hours, the inoculum size is 5%, Take the same inoculated amount of bacterial liquid and inoculate it into the basic medium II again for repeated expa...

Embodiment 3

[0045] A kind of method for producing PHB / bacterial cellulose composite material simply and quickly, comprises the steps:

[0046] (1) Microbial culture: activate the Ralstonia eutropha H16 (DSM428) strain stored in a freeze-drying tube in the seed medium, and after 24 hours of growth, inoculate the obtained bacterial solution into the basal medium Ⅰ with an inoculation amount of 5%. Expand culture to OD 600 When the value is 1, the bacterial solution was inoculated again into the basal medium I with the same inoculum amount for repeated expansion culture to its OD 600 value is 1;

[0047] (2) Activate the Gluconacetobacter xylinus ATCC700178 bacterial strain stored in the freeze-drying tube in basal medium II, and after 48 hours of growth, inoculate the obtained bacterial liquid into basal medium II for expanded culture for 24 hours, the inoculum size is 5%, Take the same inoculated amount of bacterial liquid and inoculate it into the basic medium II again for repeated expa...

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Abstract

The invention belongs to the field of biological materials, and particularly relates to a method for simply and rapidly producing a PHB / bacterial cellulose composite material. The method comprises thesteps of 1) culturing a Ralstonia eutropha bacterial solution; 2) culturing a Gluconacetobacter xylinus bacterial solution; 3) taking and adding the Ralstonia eutropha bacterial solution into a basicculture solution containing Gluconacetobacter xylinus thalli for dynamic culture and static culture, collecting a bacterial solution, and extracting the bacterial cellulose / PHB composite product. According to the method, an appropriate amount of Ralstonia eutropha bacterial solution is directly added in the Gluconacetobacter xylinus culture process so that the required composite material can be produced; the method has the advantages of being simple in operation, low in cost, mild in condition, environmentally friendly and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a simple and fast method for producing PHB / bacterial cellulose composite materials. Background technique [0002] The development of new materials is a priority scientific field in the 21st century. Bacterial cellulose (BC) is one of the research hotspots of new biomaterials nowadays. The use of microbial fermentation to produce bacterial cellulose and its application in medicine has broad application prospects in medicine, papermaking, biomedical engineering and food industry. Among them, the bacterial cellulose produced by Acetobacter is different from the cellulose that exists widely in nature, and it has the following unique properties: (1) The cellulose has high purity, high crystallinity and high degree of overlap, and exists as a single fiber, so It is very beneficial in the preparation of some microfiber products. The preparation of traditional microfibe...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12P7/62C12R1/01
Inventor 张甜胡胜军皮埃尔鲁克·川柏雷许梦莹
Owner WUHAN UNIV OF TECH