Method for detecting polysaccharides in dendrobium officinale shoots
A detection method, the technology of Dendrobium officinale, applied in the field of analytical chemical detection, to achieve the effect of shortening the extraction time, improving the quality and yield, and improving the extraction and separation rate
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Embodiment 1
[0063] Take 0.5g of powdered sample, put it in 20mL of water, ultrasonic at 40°C for 25min at 350W, then extract in a boiling water bath for 40min, cool the extract to room temperature, set the volume to 250mL, take the supernatant and refrigerate at 4°C for 2-4 hours for later use.
[0064] Take 1mL of the supernatant, add water to 2mL, add 5mL DNS, and boil in water bath for 15min, and finally put in ice water to cool for 15min, replenish water to 25mL, measure A520, and calculate the reducing sugar content of the sample according to the standard curve.
[0065] Take 1 mL of the above supernatant, add water to 2 mL, add 1 mL of 8M concentrated sulfuric acid, put in a boiling water bath for 10 min, and let cool to room temperature. Add 1 drop of phenolphthalein, adjust the pH to neutral with 40% sodium hydroxide solution, add water to 10mL, shake well, and refrigerate at 4°C for 2-4 hours. Take 2.0 mL of the mixture, add 5 mL of DNS solution, boil in water bath for 15 minutes...
Embodiment 2
[0067] Take 0.5g of powdered sample, put it in 20mL of water, ultrasonic at 50°C for 25min at 350W, then extract it in a boiling water bath for 50min, cool the extract to room temperature, and dilute to 250mL, take the supernatant and refrigerate at 4°C for 2-4 hours for later use
[0068] Take 1mL of the supernatant, add water to 2mL, add 5mL DNS, and boil in water bath for 15min, and finally put in ice water to cool for 15min, replenish water to 25mL, measure A520, and calculate the reducing sugar content of the sample according to the standard curve.
[0069] Take 1 mL of the above supernatant, add water to 2 mL, add 1 mL of 8M concentrated sulfuric acid, put in a boiling water bath for 10 min, and let cool to room temperature. Add 1 drop of phenolphthalein, adjust the pH to neutral with 40% sodium hydroxide solution, add water to 10mL, shake well, and refrigerate at 4°C for 2-4 hours. Take 2.0 mL of the mixture, add 5 mL of DNS solution, boil in water bath for 15 minutes, ...
Embodiment 3
[0071] Take 0.5g of powdered sample, put it in 20mL of water, ultrasonic at 40°C for 25min at 450W, then extract it in a boiling water bath for 60min, cool the extract to room temperature, dilute to 250mL, take the supernatant and refrigerate at 4°C for 2-4 hours, and set aside
[0072] Take 1mL of the supernatant, add water to 2mL, add 5mL DNS, and boil in water bath for 15min, and finally put in ice water to cool for 15min, replenish water to 25mL, measure A520, and calculate the reducing sugar content of the sample according to the standard curve.
[0073] Take 1 mL of the above supernatant, add water to 2 mL, add 1 mL of 8M concentrated sulfuric acid, put in a boiling water bath for 10 min, and let cool to room temperature. Add 1 drop of phenolphthalein, adjust the pH to neutral with 40% sodium hydroxide solution, add water to 10mL, shake well, and refrigerate at 4°C for 2-4 hours. Take 2.0 mL of the mixture, add 5 mL of DNS solution, boil in water bath for 15 minutes, coo...
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