Method for detecting polysaccharides in dendrobium officinale shoots

A detection method, the technology of Dendrobium officinale, applied in the field of analytical chemical detection, to achieve the effect of shortening the extraction time, improving the quality and yield, and improving the extraction and separation rate

Inactive Publication Date: 2019-05-10
苏州普朗特尔皮肤细胞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, there is no report about adopting 3,5-dinitrosalicylic acid method to determine the polysaccharide detection method of Dendrobium officinale buds

Method used

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  • Method for detecting polysaccharides in dendrobium officinale shoots
  • Method for detecting polysaccharides in dendrobium officinale shoots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Take 0.5g of powdered sample, put it in 20mL of water, ultrasonic at 40°C for 25min at 350W, then extract in a boiling water bath for 40min, cool the extract to room temperature, set the volume to 250mL, take the supernatant and refrigerate at 4°C for 2-4 hours for later use.

[0064] Take 1mL of the supernatant, add water to 2mL, add 5mL DNS, and boil in water bath for 15min, and finally put in ice water to cool for 15min, replenish water to 25mL, measure A520, and calculate the reducing sugar content of the sample according to the standard curve.

[0065] Take 1 mL of the above supernatant, add water to 2 mL, add 1 mL of 8M concentrated sulfuric acid, put in a boiling water bath for 10 min, and let cool to room temperature. Add 1 drop of phenolphthalein, adjust the pH to neutral with 40% sodium hydroxide solution, add water to 10mL, shake well, and refrigerate at 4°C for 2-4 hours. Take 2.0 mL of the mixture, add 5 mL of DNS solution, boil in water bath for 15 minutes...

Embodiment 2

[0067] Take 0.5g of powdered sample, put it in 20mL of water, ultrasonic at 50°C for 25min at 350W, then extract it in a boiling water bath for 50min, cool the extract to room temperature, and dilute to 250mL, take the supernatant and refrigerate at 4°C for 2-4 hours for later use

[0068] Take 1mL of the supernatant, add water to 2mL, add 5mL DNS, and boil in water bath for 15min, and finally put in ice water to cool for 15min, replenish water to 25mL, measure A520, and calculate the reducing sugar content of the sample according to the standard curve.

[0069] Take 1 mL of the above supernatant, add water to 2 mL, add 1 mL of 8M concentrated sulfuric acid, put in a boiling water bath for 10 min, and let cool to room temperature. Add 1 drop of phenolphthalein, adjust the pH to neutral with 40% sodium hydroxide solution, add water to 10mL, shake well, and refrigerate at 4°C for 2-4 hours. Take 2.0 mL of the mixture, add 5 mL of DNS solution, boil in water bath for 15 minutes, ...

Embodiment 3

[0071] Take 0.5g of powdered sample, put it in 20mL of water, ultrasonic at 40°C for 25min at 450W, then extract it in a boiling water bath for 60min, cool the extract to room temperature, dilute to 250mL, take the supernatant and refrigerate at 4°C for 2-4 hours, and set aside

[0072] Take 1mL of the supernatant, add water to 2mL, add 5mL DNS, and boil in water bath for 15min, and finally put in ice water to cool for 15min, replenish water to 25mL, measure A520, and calculate the reducing sugar content of the sample according to the standard curve.

[0073] Take 1 mL of the above supernatant, add water to 2 mL, add 1 mL of 8M concentrated sulfuric acid, put in a boiling water bath for 10 min, and let cool to room temperature. Add 1 drop of phenolphthalein, adjust the pH to neutral with 40% sodium hydroxide solution, add water to 10mL, shake well, and refrigerate at 4°C for 2-4 hours. Take 2.0 mL of the mixture, add 5 mL of DNS solution, boil in water bath for 15 minutes, coo...

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Abstract

The invention discloses a method for detecting polysaccharides in dendrobium officinale shoots. The method comprises the steps of making a standard curve; carrying out heat extraction, cooling and volume makeup on the dendrobium officinale shoots, and taking supernate for later use; mixing the supernate with water and DNS, carrying out heating, carrying out cooling, adding the water, measuring A520, and calculating the reducing sugar content of a sample according to the standard curve; mixing the supernate with the water and concentrated sulfuric acid, carrying out cooling after heating, adding 1 drop of phenolphthalein, adjusting the pH value to be neutral, adding the water, uniformly mixing, and carrying out refrigeration at 4 DEG C for 2 hours to obtain a mixed solution; mixing the mixed solution with the DNS solution, carrying out heating, carrying out cooling, adding the water, measuring A520, and calculating the total sugar content of the sample according to the standard curve; and obtaining the content of the polysaccharides by subtracting the reducing sugar content from the total sugar content of the sample. The detection method provided by the invention is small in error,relatively high in speed and convenient to operate.

Description

technical field [0001] The invention relates to the field of analytical chemistry detection, in particular to a detection method for polysaccharides in dendrobium officinale buds. Background technique [0002] Dendrobium officinale (Dendrobium officinale) is a perennial herbal Chinese herbal medicine plant. It is recorded in the Chinese Pharmacopoeia (2015 edition). , hot stranguria astringent pain and carbuncle swollen furunculosis and other effects. Similarly, modern medical research shows that Dendrobium candidum has the function of enhancing immune T cells, which can improve human immunity, remove aging and mutated cells, stabilize blood sugar, increase cell metabolism rate, and maintain the physiological stability of the body. [0003] Although Dendrobium candidum has been artificially planted on a large scale, its annual output can only meet the needs of one province due to its slow growth and small planting area. The use of plant tissue culture technology can not on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/34G01N1/38
Inventor 张杰王昕怡吴红升
Owner 苏州普朗特尔皮肤细胞科技有限公司
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