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A kind of plasma-activated lactic acid solution with high antibacterial performance and its preparation method and application

A technology of plasma and lactic acid liquid, which is applied in the application, preservation of meat/fish with chemicals, food processing, etc., can solve the problems of 30 to 60 minutes, small amount of liquid treatment, long treatment time, etc., and achieve improved antibacterial effect , stable antibacterial activity, and low energy consumption for preparation

Active Publication Date: 2022-01-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, plasma treatment liquids mainly include deionized water, phosphate buffer solution, hydrogen peroxide solution, etc. The main problems of these methods are: 1) The amount of liquid treatment is small, and generally 96-well plates, petri dishes or 50mL beakers are used to hold 2) The processing time is long, most of them need to be processed for about 10 minutes, and some processing time even reaches 30 to 60 minutes; 3) The processing efficiency is low, the ratio of plasma activation solution to bacterial solution needs to be 9:1, or even 99 :1

Method used

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  • A kind of plasma-activated lactic acid solution with high antibacterial performance and its preparation method and application
  • A kind of plasma-activated lactic acid solution with high antibacterial performance and its preparation method and application
  • A kind of plasma-activated lactic acid solution with high antibacterial performance and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The water of 300mL and the lactic acid solution (0.05%, 0.10%, 0.15% and 0.20%) of different mass concentrations were treated with a plasma spray gun for 0, 40, 60, and 80 s respectively, and stood still at room temperature for 20 min (the active solution after processing was The temperature reaches room temperature to avoid the influence of temperature on the antibacterial results). Combine the treated plasma activated water and activated lactic acid with 10 8 The CFU / mL Salmonella enteritidis suspension was reacted at a ratio of 1:1 for 10 minutes. The antibacterial properties of different plasma activation solutions were analyzed by plate counting, and the results were as follows: figure 2 shown, figure 2 The results showed that the antibacterial effect of plasma-activated lactic acid was significantly better than that of plasma-activated water. When the concentration of lactic acid solution is 0.05, 0.10, 0.15 and 0.20%, after plasma jet treatment for 100s, the...

Embodiment 2

[0029] Plasma-activated water and activated lactic acid (with different lactic acid concentrations) with the same plasma treatment time were placed at room temperature for 7 days, and the changes in antibacterial activity of different plasma-activated solutions during storage were studied. The results are as follows: image 3 shown, image 3 The results showed that the plasma-activated lactic acid solution still had a similar antibacterial effect to the freshly treated plasma-activated water after being left for 3 days, while the plasma-activated water lost its antibacterial effect after only one day of storage.

Embodiment 3

[0031] 300 mL of water and different concentrations of lactic acid solutions (0.05%, 0.10%, 0.15% and 0.20%) were treated with a plasma spray gun for 80 s, and then stood still at room temperature for 20 min. The chicken wings were immersed in different plasma activation solutions for 10 minutes, and water, 0.05% sodium hypochlorite solution and 2.0% lactic acid solution were used as control groups. After the treatment, the changes in color, pH, lipid oxidation and microorganisms of the samples were detected, and the results are shown in Table 1.

[0032] Table 1 Effects of plasma-activated lactic acid soaking treatment on the surface color value, lipid oxidation and antibacterial effect of chicken wings

[0033]

[0034] The data in Table 1 shows that after chicken wings are treated with plasma activation solution, compared with water treatment and existing process treatment, L * and b * There were no significant differences in lipid oxidation and lipid oxidation. a of ...

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Abstract

The invention relates to a plasma-activated lactic acid solution with high-efficiency antibacterial performance, a preparation method and application thereof, and belongs to the field of materials. In this method, the plasma jet is used as the generating device, and air is used as the working gas. After the air enters the generating device, it passes through the high-voltage electrode for treatment. After the treatment, the plasma flows out from the nozzle of the spray gun and enters the low-concentration lactic acid solution to complete the preparation of the activated lactic acid solution. The activated lactic acid solution prepared by the method has high-efficiency bactericidal performance, short preparation time, high efficiency, stable bactericidal performance, low energy consumption and no pollution, and is especially suitable for cleaning and disinfecting products in the slaughtering process of livestock and poultry and cleaning and sterilizing fruits and vegetables.

Description

technical field [0001] The invention relates to the field of food. In particular, it relates to a preparation method of a plasma-activated lactic acid liquid with high antibacterial performance, a preparation method and an application thereof. Background technique [0002] Foodborne pathogens are one of the main causes of food safety problems, not only causing serious harm to human health, but also causing huge economic losses to the meat industry. For poultry meat, sodium hypochlorite solution is currently a commonly used method in China, but the European Union believes that its by-products may cause cancer, which has a huge impact on the export of poultry meat in my country. For red meat such as cattle, sheep, and pigs, domestic slaughterhouses mostly use high-pressure water spraying or use 5%-2% high-concentration lactic acid solution. Among them, high-pressure water spray washing mainly uses the impact force of water to remove bacteria from the surface, but there will ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B4/20A23L3/015A23L3/3508
CPCY02P60/85
Inventor 章建浩严文静钱婧
Owner NANJING AGRICULTURAL UNIVERSITY
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