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Method for preparing perilla peptide

The technology of perilla peptide and perilla meal is applied in the field of preparation of perilla peptide, which can solve the problems of waste of resources and low utilization rate of nutrients, and achieve the effects of reducing waste, being easy to absorb, and improving utilization rate.

Active Publication Date: 2019-05-17
湖北瑞邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Perilla meal is used for feed, fertilizer or directly discarded, and the utilization rate of nutrients in perilla meal is not high, resulting in a waste of resources

Method used

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  • Method for preparing perilla peptide

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[0015] The embodiment of the present invention provides a preparation method of perilla peptide, comprising the following steps:

[0016] (1) After the pulverized perilla meal is subjected to denaturation treatment, the perilla meal protein liquid is obtained;

[0017] (2) The perilla meal protein solution in step (1) is enzymolyzed by α-amylase, alkaline protease and compound protease in sequence to obtain an enzymatic hydrolysis product;

[0018] (3) The enzymatic hydrolysis product in step (2) is subjected to enzyme inactivation, decolorization, concentration and freeze-drying to obtain a finished product.

[0019] In the present invention, the denaturation treatment of perilla meal adopts the following method: after mixing the pulverized perilla meal with water, heat it at 80-85°C for 1-2 hours, wherein the mass ratio of perilla meal to water is 1:15- 20.

[0020] In the present invention, the perilla meal is defatted perilla meal, and the defatted perilla meal is crushe...

Embodiment 1

[0034] Embodiment 1 of the present invention provides a kind of preparation method of perilla peptide, comprises the following steps:

[0035] (1) Grinding the defatted perilla meal until the particle size is less than 100um, mixing the obtained perilla meal powder with distilled water at a mass ratio of 1:18, and heating the mixture in a water bath at 82°C for 1 hour to obtain a perilla meal protein liquid;

[0036] (2) Adjust the pH of the perilla meal protein liquid in step (1) to 4.5, raise the temperature to 70° C., add α-amylase with a dry weight of perilla meal protein in an amount of 2 wt%, stir and mix evenly, and perform enzymatic hydrolysis for 2 hours , to obtain α-amylase hydrolysis product;

[0037] (3) Adjust the pH of the α-amylase hydrolyzate in step (2) to 8.5, and the temperature rises to 55° C., add Alcalase 2.4L alkaline protease with 2wt% dry weight of the α-amylase hydrolyzate, and stir After mixing evenly, enzymolyze for 3 hours to obtain alkaline prot...

Embodiment 2

[0041] Embodiment 2 of the present invention provides a kind of preparation method of perilla peptide, comprises the following steps:

[0042] (1) Crush the defatted perilla meal to a particle size of less than 100um, mix the obtained perilla meal powder with distilled water at a mass ratio of 1:15, and heat the mixture in a water bath at 80°C for 1.5 hours to obtain a perilla meal protein solution ;

[0043] (2) Adjust the pH of the perilla meal protein solution to 4 in step (1), raise the temperature to 65° C., and add α-amylase with a dry weight of perilla meal protein of 2 wt%, stir and mix evenly, and enzymolyze 2.5 h, to obtain α-amylase hydrolysis product;

[0044] (3) The pH of the α-amylase hydrolyzate in step (2) is adjusted to 8, the temperature rises to 50° C., and Alcalase 2.4L alkaline protease with a dry weight of 1.5 wt% of the α-amylase hydrolyzate is added, After stirring and mixing evenly, enzymatic hydrolysis for 3 hours to obtain the hydrolyzate of alkal...

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Abstract

The invention discloses a method for preparing perilla peptide. The method includes step (1) of performing denaturating treatment on smashed perilla meal to obtain perilla meal protein liquid; step (2) of performing enzymolysis on the perilla meal protein liquid sequentially through alpha-amylase, alkaline protease and complex protease to obtain an enzymolysis product; and step (3) of subjecting the enzymolysis product to inactivation, decolorization, concentration and freeze-drying to obtain a finished product. The perilla meal is subjected to the denaturating treatment, enzymolysis through the different enzymes and freeze-drying and the like, so that high-quality protein in the perilla meal is converted into small molecule active peptide which is easily absorbed by the body. The method not only extracts nutrients in the perilla meal, but also improves the perilla utilization rate, and reduces the waste of resources.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method of perilla peptide. Background technique [0002] Perilla, also known as Guiren, Baisu, Chisu, is an annual herb of Lamiaceae, which has a specific aroma. Perilla leaves are a delicious condiment widely used in the Wuchuan area of ​​Zhanjiang in southern China. Perilla seed is the dry and mature fruit of perilla, which is widely used clinically and is also an important economic oil crop. Perilla seeds are warm in nature and non-toxic. Eating raw can quench thirst and nourish the lungs; cooked food can nourish the middle and nourish qi, eliminate phlegm and relieve cough, lower blood pressure, and calm asthma. [0003] At present, perilla seeds are widely used to extract oil to obtain perilla seed oil, and the by-product perilla meal after oil extraction is generally used for feed, fertilizer or directly discarded. However, perilla meal i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J3/34
Inventor 靳祯亮尹晓清王希搏吴海刘雅颀
Owner 湖北瑞邦生物科技有限公司