A digestive system inflammation drug targeted delivery drug system
A technology of digestive system and inflammation, applied in the field of designing targeted drugs for inflammation, can solve the problems of drug internal organ toxicity, drug consumption, etc., and achieve the effect of strong targeting, improving therapeutic effect, and applying drugs accurately
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preparation example Construction
[0048] The preparation method of the system A is as follows:
[0049] Step A-1 Pretreatment of neutrophils: take the freshly prepared neutrophil suspension, incubate in the culture medium at 37°C for 1-5 hours, and remove the culture medium;
[0050] Add positively charged liposomes to the incubated neutrophils, add fresh culture medium to dilute, and incubate at 37°C for 1-2 hours;
[0051] Step A-2 Encapsulation of the first base sequence: Take the prepared first base sequence fragment, add fresh culture medium to dilute and negatively charged liposomes, ultrasonically disperse, and incubate at 37°C for 1-3 hours ;
[0052] Step A-3 connection of neutrophils and the first nucleotide sequence, the incubated neutrophils obtained in step A-1 and the incubated first nucleotide sequence fragments obtained in step A-2 are positively charged Liposomes and negatively charged liposomes are mixed at a mass ratio of 2-4:1, and incubated at 37° C. for 2 hours after mixing; neutrophils...
Embodiment 1
[0072] System A was prepared as follows:
[0073] Step A-1 Pretreatment of neutrophils: Take the freshly prepared neutrophil suspension, incubate in the culture medium at 37°C for 1 hour, and remove the culture medium;
[0074] Add positively charged liposomes to the incubated neutrophils, add fresh culture medium to dilute, and incubate at 37°C for 2 hours;
[0075] Step A-2 Encapsulation of the first base sequence: take the prepared first base sequence fragment, add fresh culture medium to dilute and negatively charged liposomes, ultrasonically disperse, and incubate at 37°C for 3 hours;
[0076] Step A-3 connection of neutrophils and the first nucleotide sequence, the incubated neutrophils obtained in step A-1 and the incubated first nucleotide sequence fragments obtained in step A-2 are positively charged Liposomes and negatively charged liposomes were mixed at a mass ratio of 2:1, and incubated at 37° C. for 2 hours after mixing; neutrophils connected with the first base...
Embodiment 2
[0092] System A was prepared as follows:
[0093] Step A-1 Pretreatment of neutrophils: take the freshly prepared neutrophil suspension, incubate in the culture medium at 37°C for 2 hours, and remove the culture medium;
[0094] Add positively charged liposomes to the incubated neutrophils, add fresh culture medium to dilute, and incubate at 37°C for 1 hour;
[0095] Step A-2 Encapsulation of the first base sequence: take the prepared first base sequence fragment, add fresh culture medium to dilute and negatively charged liposomes, ultrasonically disperse, and incubate at 37°C for 2 hours;
[0096] Step A-3 connection of neutrophils and the first nucleotide sequence, the incubated neutrophils obtained in step A-1 and the incubated first nucleotide sequence fragments obtained in step A-2 are positively charged Liposomes and negatively charged liposomes were mixed at a mass ratio of 2:1, and incubated at 37° C. for 2 hours after mixing; neutrophils connected with the first base...
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