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4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress and preparation method thereof

A technology of endoplasmic reticulum stress and phenylbutyric acid, applied in the direction of tumor/cancer cells, vertebrate cells, animal cells, etc., can solve the problems of cell cytotoxicity and cell damage, achieve good repeatability, reduce Toxicity, destabilizing effects

Active Publication Date: 2019-05-21
AFFILIATED HOSPITAL OF ZUNYI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of endoplasmic reticulum inducer and PBA can explore the molecular mechanism of endoplasmic reticulum stress more fully, but the method for preparing PBA solution in the prior art is to adopt water bath to make 4-phenylbutyric acid obtain PBA solution, but this The solution is acidic and has obvious cytotoxic effect on cells. After adjusting the pH value to the optimum pH value for cell culture with sodium bicarbonate or sodium hydroxide, it still has obvious damage to cells.

Method used

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  • 4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress and preparation method thereof
  • 4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress and preparation method thereof
  • 4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress and preparation method thereof

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preparation example Construction

[0020] The invention provides a method for preparing a 4-phenylbutyric acid solution that relieves endoplasmic reticulum stress, comprising the following steps:

[0021] 1) Mix 4-phenylbutyric acid with serum-free DME / F-12 medium, and vortex the obtained blend in a water bath at 48-52°C for 8-12 minutes to obtain a pretreatment solution;

[0022] 2) Let the pretreatment solution in step 1) stand for 22-26 hours to obtain a 4-phenylbutyric acid solution that relieves endoplasmic reticulum stress.

[0023] In the present invention, 4-phenylbutyric acid is mixed with serum-free DME / F-12 medium, and the obtained blend is vortexed in a water bath at 48-52° C. for 8-12 minutes to obtain a pretreatment liquid. In the present invention, the mass volume ratio of the 4-phenylbutyric acid to the serum-free DME / F-12 medium is preferably 32.8mg:20mL. Serum-free DME / F-12 medium is the basal medium for HepG2 culture. In the present invention, the serum-free DME / F-12 medium can cultivate cel...

Embodiment 1

[0033] Weigh 32.8 mg of 4-phenylbutyric acid, add it to 20 mL of serum-free DME / F-12 medium, vortex in a water bath at 52°C for 8 min, and turn orange to obtain a pretreatment solution. The pretreatment solution was left standing for 22 hours under ultraviolet irradiation on a clean bench, and it turned red, and then sterilized with a filter with a filter membrane pore size of 0.22 μm to obtain a 4-phenylbutyric acid solution that relieves endoplasmic reticulum stress.

Embodiment 2

[0035] Weigh 32.8 mg of 4-phenylbutyric acid, add it to 20 mL of serum-free DME / F-12 medium, vortex in a water bath at 48°C for 12 min, and turn orange to obtain a pretreatment solution. The pretreatment solution was left to stand for 26 hours under ultraviolet irradiation on a clean bench, and it turned red, and then sterilized with a filter with a filter membrane pore size of 0.22 μm to obtain a 4-phenylbutyric acid solution that relieves endoplasmic reticulum stress.

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Abstract

The invention provides a 4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress and a preparation method thereof, and belongs to the technical field of solvent preparation. Themethod comprises the following steps that 1), 4-phenylbutyric acid is mixed with a serum-free DMF / F-12 culturing medium, an obtained mixture eddies for 8-12 min in water bath at the temperature of 48-52 DEG C, and pretreatment liquid is obtained; 2), the pretreatment liquid in the step 1) is subjected to standing for 22-26 h, and the 4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress is obtained. The DMF / F-12 culturing medium containing the 4-phenylbutyric acid is placed in air to alkalify the solution, damage of acidic substances to cells is balanced, and therefore the prepared 4-phenylbutyric acid solution capable of reducing endoplasmic reticulum stress can reduce toxicity to the cells.

Description

technical field [0001] The invention belongs to the technical field of solvent preparation, and in particular relates to a 4-phenylbutyric acid solution for alleviating endoplasmic reticulum stress and a preparation method thereof. Background technique [0002] The endoplasmic reticulum is an important membranous reticular organelle in cells, which is mainly involved in the synthesis and metabolism of proteins, lipids and sugars, and regulates the storage and release of calcium ions. Among the cells of various tissues and organs in the human body, the endoplasmic reticulum in liver cells is the most abundant. When the body has physiological or pathological factors (nutrition deficiency, acidosis, virus infection, etc.), the homeostasis of the endoplasmic reticulum can be disrupted. Once the homeostasis of the endoplasmic reticulum is disrupted, sudden changes in the calcium ion level in the endoplasmic reticulum and accumulation of misfolded and unfolded proteins can induce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
Inventor 何毅怀陈欢唐永静汪杰沈访
Owner AFFILIATED HOSPITAL OF ZUNYI UNIV
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