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Quick environmental-friendly detection method of free fatty acid methyl ester and ethyl ester of olive oil

A technology of fatty acid methyl ester and detection method, which can be applied to measurement devices, material separation, instruments, etc., can solve problems such as infringing on consumers' rights and interests, affecting consumers' health, etc., and achieves saving in the use of organic solvents, high sensitivity, and processing process. easy effect

Inactive Publication Date: 2019-05-24
南京海关动植物与食品检测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This shoddy olive oil not only seriously violates the rights and interests of consumers, but may also affect the health of consumers

Method used

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  • Quick environmental-friendly detection method of free fatty acid methyl ester and ethyl ester of olive oil
  • Quick environmental-friendly detection method of free fatty acid methyl ester and ethyl ester of olive oil
  • Quick environmental-friendly detection method of free fatty acid methyl ester and ethyl ester of olive oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 establishes the detection method of free fatty acid methyl ester and ethyl ester in olive oil

[0027] 1. Preparation of standard stock solution

[0028] Accurately weigh methyl myristate (Methyl myristate, CAS No.: 124-10-7, denoted as C14:0M), methyl palmitate (Methyl palmitate, CAS No.: 112-39-0, denoted as C16:0M ), ethyl palmitate (Ethylpalmitate, CAS No.: 628-97-7, denoted as C16:0E), methyl heptadecanoate (Methyl heptadecanoate, CAS No.: 1731-92-6, denoted as C17:0M) , Methyl linoleate (Methyl linoleate, CAS No.: 112-63-0, denoted as C18:2M), Methyl oleate (Methyl oleate, CAS No.: 112-62-9, denoted as C18:1M), Methylstearate (Methylstearate, CAS No.: 112-61-8, denoted as C18:0M), ethyl linoleate (Ethyl linoleate, CAS No.: 544-35-4, denoted as C18:2E), oil Ethyl oleate (CAS No.: 111-62-6, denoted as C18:1E) and ethyl stearate (Ethylstearate, CAS No.: 111-61-5, denoted as C18:0E) 1mg each (accurate to 0.01mg), placed in a 10mL volumetric flask, diss...

Embodiment 2

[0055] Embodiment 2 adopts the inventive method to detect the recovery and precision of fatty acid methyl ester and ethyl ester content in the olive oil sample

[0056] 24 samples of 0.05 g each were taken from the same extra virgin olive oil sample and divided into 4 groups of 6 samples each. Group 1 was pretreated according to Title 3 of Example 1, and then tested according to the method of Title 4 of Example 1; Groups 2-4 were used for standard addition recovery test, and 25 μL of mixed standard was added to each sample of Group 2 Standard working solution I (Title 2 of Example 1, concentration is 10 μg / mL), add 50 μL mixed standard working solution I (Title 2 of Example 1, concentration is 10 μg / mL) to each sample of the third group, in Add 100 μL mixed standard working solution I (Title 2 of Example 1, concentration is 10 μg / mL) to each sample of Group 4, then use Title 3 of Example 1 for pretreatment, and test with the method in Title 4 of Example 1 .

[0057] Compare ...

Embodiment 3

[0065] Embodiment 3 adopts the inventive method to detect fatty acid methyl ester and ethyl ester content in the olive oil sample

[0066] The method in Title 7 of Example 1 was used to determine the content of free fatty acid methyl ester and ethyl ester in 4 kinds of olive oil samples in the laboratory edible vegetable oil sample bank. Two samples were taken from each kind of olive oil, and each sample was measured three times to obtain the average value. The specific measurement results are shown in Table 3 and Figure 2-5 .

[0067] from Figure 2-5 Visible, the olive oil sample exists with figure 1 C16:0M, C16:0E, C18:2M, C18:1M, C18:0M, C18:2E, C18:1E, and C18:0E have the same retention time, which can be qualitative. In addition, the olive oil sample also has substances with the same retention time as C14:0M and C17:0M in the mixed standard working solution II, which proves that the detection method has good stability and high reliability.

[0068] Table 3 The conte...

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Abstract

The invention provides a quick environmental-friendly detection method of free fatty acid methyl ester and ethyl ester of olive oil and belongs to the field of food detection. The quick environmental-friendly detection method includes the steps of (1) subjecting an olive oil sample to pretreatment by silicagel column Supelclean TMLC-Si SPE; (2), analyzing the pretreated olive oil by gas chromatography; (3) calculating content of free fatty acid methyl ester and ethyl ester of the olive oil according to working curves. According to the detection method, the pretreatment process is simple and quick, a great quantity of organic solvents harmful to human body can be saved, low cost and environmental friendliness ca be achieved; besides, the detection method needs short time for analysis, is accurate and reliable and has high flexibility.

Description

technical field [0001] The invention relates to a rapid and environment-friendly detection method for free fatty acid methyl ester and ethyl ester in olive oil, belonging to the field of food detection, in particular to the field of food adulteration detection. Background technique [0002] Olive oil is a fat made from the fruit of the olive tree. According to different processing techniques, it can be divided into virgin olive oil and pomace oil, and virgin olive oil can be divided into different grades, among which extra virgin olive oil has the highest nutritional value. my country is a large edible oil country. With economic development, my country's demand for olive oil continues to increase. In 2017 alone, the total consumption was about 600,000 tons, of which 80% depended on imports. [0003] However, Chinese consumers have limited knowledge of olive oil series products, and the production of extra virgin olive oil is small and the price is high. It is not uncommon ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 陆慧媛沈伟健吴斌姜珊王红胡国绅丁涛
Owner 南京海关动植物与食品检测中心
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