Fluorescent microsphere joint examination device for five analytes of thyroid and preparation method thereof

A fluorescent microsphere and combined detection technology, which is applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of easy quenching of fluorescent antibodies, insufficient amount of protein adsorbed by NC membrane, and weak binding force, and achieve good biological performance. Compatibility, improved coating efficiency, high mechanical strength effect

Inactive Publication Date: 2019-05-24
吉林双正生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a fluorescent microsphere joint detection device for five items of methazine and a preparation method thereof, so as to solve the problems in the prior art that the fluorescent antibody is easily quenched, the amount of protein adsorbed by the NC membrane is insufficient, and the binding force is not strong

Method used

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  • Fluorescent microsphere joint examination device for five analytes of thyroid and preparation method thereof
  • Fluorescent microsphere joint examination device for five analytes of thyroid and preparation method thereof
  • Fluorescent microsphere joint examination device for five analytes of thyroid and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A combined detection device for fluorescent microspheres with five items of methazine, which is composed of the following five detection devices:

[0050] Detection device one 100: sample pad one 101, immunofluorescence cross-linked thyroxine (T4) antibody glass fiber membrane 102, nitrocellulose membrane 103, and absorption pad 104 are respectively pasted on a plastic plate 105, and the two sides of the nitrocellulose membrane 103 The other end of the immunofluorescent cross-linked thyroxine (T4) antibody glass fiber membrane 102 is overlapped with the sample pad one 101 respectively. ; The detection line T is set on the nitrocellulose membrane 103 T4 and quality control line C, the test line T T4 Mark thyroxine (T4) antibody; Spray point goat anti-mouse IgG polyclonal antibody on the described quality control line C;

[0051] Detection device two 200: sample pad one 201, immunofluorescence cross-linked triiodothyronine (T3) antibody glass fiber membrane 202, nitroce...

Embodiment 2

[0077] A combined detection device for fluorescent microspheres with five items of methazine, which is composed of the following five detection devices:

[0078] Detection device one 100: sample pad one 101, immunofluorescence cross-linked thyroxine (T4) antibody glass fiber membrane 102, nitrocellulose membrane 103, and absorption pad 104 are respectively pasted on a plastic plate 105, and the two sides of the nitrocellulose membrane 103 The other end of the immunofluorescent cross-linked thyroxine (T4) antibody glass fiber membrane 102 is overlapped with the sample pad one 101 respectively. ; The detection line T is set on the nitrocellulose membrane 103 T4 and quality control line C, the test line T T4 Mark thyroxine (T4) antibody; Spray point goat anti-mouse IgG polyclonal antibody on the described quality control line C;

[0079] Detection device two 200: sample pad one 201, immunofluorescence cross-linked triiodothyronine (T3) antibody glass fiber membrane 202, nitroce...

Embodiment 3

[0105] A combined detection device for fluorescent microspheres with five items of methazine, which is composed of the following five detection devices:

[0106] Detection device one 100: sample pad one 101, immunofluorescence cross-linked thyroxine (T4) antibody glass fiber membrane 102, nitrocellulose membrane 103, and absorption pad 104 are respectively pasted on a plastic plate 105, and the two sides of the nitrocellulose membrane 103 The other end of the immunofluorescent cross-linked thyroxine (T4) antibody glass fiber membrane 102 is overlapped with the sample pad one 101 respectively. ; The detection line T is set on the nitrocellulose membrane 103 T4 and quality control line C, the test line T T4 Mark thyroxine (T4) antibody; Spray point goat anti-mouse IgG polyclonal antibody on the described quality control line C;

[0107]Detection device two 200: sample pad one 201, immunofluorescence cross-linked triiodothyronine (T3) antibody glass fiber membrane 202, nitrocel...

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Abstract

The invention relates to a fluorescent microsphere joint examination device for five analytes of thyroid and a preparation method thereof and belongs to the field of medical examination equipment. A sample pad, an immunofluorescence crosslinked antibody glass fiber membrane, a nitrocellulose membrane and an absorbing pad are bonded to a plastic board; two ends of the nitrocellulose membrane are inlap joint respectively with the absorbing pad and the immunofluorescence crosslinked antibody glass fiber membrane; the other end of the immunofluorescence crosslinked antibody glass fiber membrane is in lap joint with the sample pad; the nitrocellulose membrane is provided with a detection line T and a quality control line C; thyroxine antibody, thyronine antibody and hormothyrin antibody are marked on the detection line; goat anti-rat IgG polyclonal antibody is spotted on the quality control line C. The fluorescent microsphere joint examination device for five analytes of thyroid and the preparation method thereof have the advantages that thyroxine, thyronine and hormothyrin antibodies are applied to coat the nitrocellulose membrane, high specificity is achieved, T3 antibody, T4 antibody, TSH antibody, free T3 antibody and free T4 antibody can be simultaneously detected in a specimen, and the device herein is simple to operate and highly practical.

Description

technical field [0001] The present invention relates to the field of medical detection equipment, in particular to a combined detection device for the five thyroid functions and a preparation method thereof, which uses fluorescence immunochromatography technology to quantitatively detect human thyroxine (T4) in clinical specimens (whole blood / serum / plasma) , triiodothyronine (T3), thyroid-stimulating hormone (TSH), free T3, free T4 detection device and preparation method thereof, which can realize sensitive, specific and rapid detection of thyroid function markers. Background technique [0002] Thyroid disease is a common clinical disease. Such diseases mainly include hypothyroidism and hyperthyroidism. Hypothyroidism is also known as "hypothyroidism". This disease is mostly caused by too little thyroid hormone secreted by the thyroid gland. The main clinical manifestations of this disease are fear of cold, loss of memory and appetite, and mild anemia. Hyperthyroidism is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/68G01N33/533
Inventor 李志兴赵旻李欣
Owner 吉林双正生物工程有限公司
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