Kit for preparing disease-treating agent, disease-treating agent and method for preparing disease-treating agent

A disease treatment and kit technology, applied in biochemical equipment and methods, chemical instruments and methods, sensory diseases, etc., can solve the problems of not being used to treat heart failure, unable to cope with emergency surgery, cost and time, etc. Not easy to reject, stable and constant effect, easy to commercialize effect

Pending Publication Date: 2019-05-24
ROHTO PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are disadvantages such as the cost and time required for preparation, making it impossible to cope with emergency surgery, and the characteristics of the prepared cell sheet due to the difference of each patient due to the use of cells derived from one's own tissue, and the obtained effect cannot be said to be constant. of; etc.
However, this fibrin gel is a substance that forms a coating on the surface of the affected part by spraying fibrinogen and thrombin as precursors on the surface of the affected part, and although it can suppress leakage and bleeding of the infiltrate from the affected part, it can be expected to be used Tissue damage healing effect of the active ingredient, but there is no example of use in the treatment of severe heart failure caused by ischemic cardiomyopathy

Method used

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  • Kit for preparing disease-treating agent, disease-treating agent and method for preparing disease-treating agent
  • Kit for preparing disease-treating agent, disease-treating agent and method for preparing disease-treating agent
  • Kit for preparing disease-treating agent, disease-treating agent and method for preparing disease-treating agent

Examples

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preparation example Construction

[0060] (Method for preparing mesenchymal stem cells)

[0061] The mesenchymal stem cells can be prepared by a method known to those skilled in the art. The method for preparing adipose tissue-derived mesenchymal stem cells will be described below as an example. Adipose tissue-derived mesenchymal stem cells can be obtained by, for example, the production method described in US Patent No. 6,777,231, for example, can be produced by a method including the following steps (i) to (iii):

[0062] (i) a process of obtaining a cell suspension by enzymatically digesting adipose tissue;

[0063] (ii) a process of pelleting the cells and resuspending the cells in a suitable medium; and

[0064] (iii) A step of culturing cells on a solid surface and removing cells that do not appear to be bound to the solid surface.

[0065] The adipose tissue used in the step (i) is preferably washed adipose tissue. Washing can be performed by using a physiologically acceptable saline solution (such a...

Embodiment

[0108] The present invention will be specifically described by the following examples, but the present invention is not limited to the examples.

[0109] [1] Preparation of the disease therapeutic agent of the present invention

[0110] Preparation of adipose tissue-derived mesenchymal stem cells

[0111] After obtaining the consent of the human donor, the subcutaneous fat tissue obtained by liposuction was washed with physiological saline. In order to destroy the extracellular matrix and separate the cells, collagenase (Roche) was added (the solvent was physiological saline), and the mixture was dispersed by shaking at 37° C. for 90 minutes. Next, the suspension was centrifuged at 800 g for 5 minutes to obtain a precipitate of interstitial vascular cell population. Serum-free medium for mesenchymal stem cells (Rohto Co.) was added to the above cell pellet, the cell suspension was centrifuged at 400 g for 5 minutes, the supernatant was removed, and then suspended in serum-...

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Abstract

The present invention addresses the problem of providing a disease-treating agent which exerts an excellent effect in treating diseases requiring an emergency surgery such as heart failure and which is efficacious at a certain level for a large number of patients. The present invention pertains to a kit for preparing a disease-treating agent, said kit comprising a) a fibrinogen solution, b) a thrombin solution and c) mesenchymal stem cells, in separate forms respectively. It is preferred that the mesenchymal stem cells c) are allogeneic to a subject to be treated. Also, the present invention pertains to a kit for preparing a disease-treating agent, said kit being to be used by, before using, suspending the mesenchymal stem cells c) in either the fibrinogen solution a) or the thrombin solution b), and then spraying the cell suspension thus obtained directly to a disease site substantially simultaneously with the thrombin solution b) or fibrinogen solution a) that is not used in the suspending step.

Description

technical field [0001] The invention relates to a kit for preparing a disease treatment agent, a disease treatment agent and a preparation method of the disease treatment agent. Background technique [0002] For the treatment of severe heart failure caused by ischemic cardiomyopathy, heart transplantation is considered to be the most effective method, but the problem of shortage of donors is very serious. In addition, although the use of an artificial heart is also an option, it has been pointed out that there are complications such as infection and cerebral thrombosis. On the other hand, myocardial regeneration therapy using skeletal muscle myoblast sheet using own cells is attracting attention as a new therapeutic method at present (see Patent Document 1). Cardiac regeneration therapy using this skeletal muscle myoblast sheet is to prepare a sheet of myoblasts collected from the patient's own skeletal muscle and paste it on the surface of the heart suffering from heart fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/10A61K9/12A61K38/00A61K38/48A61P9/00A61P27/02A61P43/00
CPCA61K9/10A61K9/12A61K35/28A61K38/48A61P9/00A61P27/02A61P43/00C07K14/75A61K38/363A61K38/4833C12N5/0667C12N9/6429C12Y304/21005A61K2300/00A61P9/10A61K9/06A61K9/08A61K38/00
Inventor 泽芳树宫川繁梶田大辅玉田琴绘
Owner ROHTO PHARM CO LTD
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