Composite plant extract, preparation method and application
A kind of plant extract, extract technology, applied in the direction of application, food science, food preservation, etc., to achieve the effect of prolonging the storage period, ensuring human health, and high-efficiency antibacterial effect
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Embodiment 1
[0020] Antibacterial test of compound plant extract:
[0021] (1) Experimental bacteria (bacteria name is italic)
[0022] Food-borne pathogens:
[0023] Pseudomonadaceae Fluorescens ATCC 13525;
[0024] Salmonella enteritidis (Salmonella enteritidis CMCC(B)50335);
[0025] Proteus species (proteus species CMCC(B)49027);
[0026] Escherichia coli (E.coli k-12IFO 3301);
[0027] Listeria monocytogenes (Listeria monocytogenes ATCC 19115);
[0028] Staphylococcus aureus (Staphylococcus aureus CMCC(B)26003)
[0029] (2) Experimental method
[0030] 2.1 Compound plant extracts
[0031] Prepare clove, rosemary, licorice, and oregano, crush the prepared cloves, rosemary, licorice, and oregano separately, soak them in 99.5% absolute ethanol, and extract the quality of each plant The volume ratio is 1:9 (m / v), soaking and shaking for 40-52h, preferably, under the condition of 130-170rpm / min, soaking and shaking for 40-52h, specifically in this embodiment, at 150rpm / Under the condition of min, soak ...
Embodiment 2
[0052] Antibacterial test of a single plant extract:
[0053] (1) Experimental bacteria (bacteria name is italic)
[0054] Food-borne pathogens:
[0055] Pseudomonadaceae Fluorescens ATCC 13525;
[0056] Salmonella enteritidis (Salmonella enteritidis CMCC(B)50335);
[0057] Proteus species (proteus species CMCC(B)49027);
[0058] Escherichia coli (E.coli k-12 IFO 3301);
[0059] Listeria monocytogenes (Listeria monocytogenes ATCC 19115);
[0060] Staphylococcus aureus (Staphylococcus aureus CMCC(B)26003)
[0061] (2) Experimental method
[0062] 2.1 Single plant extract
[0063] Prepare cinnamon, cloves, sage, rosemary, licorice, thyme, star anise, oregano. Combine the prepared cinnamon, cloves, sage, rosemary, licorice, thyme, star anise, oregano. After pulverization, they were soaked and extracted with 99.5% absolute ethanol. The ratio of the mass of each plant to the volume of ethanol was 1:9 (m / v). Under the condition of 150rpm / min, soak and shake for 48h, then filter Obtain the filtrat...
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