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Application of wedelolide in preparation of medicine for treating fungal keratitis

A technology of fungal keratitis and wecarolide, which is applied in the pharmaceutical field, can solve the problems of unsatisfactory effects of drugs and surgical treatment of fungal keratitis, etc.

Active Publication Date: 2022-02-22
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the effect of drug and surgical treatment of fungal keratitis is not very satisfactory

Method used

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  • Application of wedelolide in preparation of medicine for treating fungal keratitis
  • Application of wedelolide in preparation of medicine for treating fungal keratitis
  • Application of wedelolide in preparation of medicine for treating fungal keratitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 蟛蜞蟛蜞 内 内 inhibitory human macrophane secretion IL-1β

[0027] Experimental material

[0028] 1.1 Experimental Drugs: 蟛蜞蟛蜞 内 (purchased from SELLECK)

[0029] 1.2 Experimental cells: human THP-1 cells (China Typical Casting Center)

[0030] 1.3 Experimental Fungi: 3.0,772 Tobacco Mildew (China Ordinary Microbial Square Savings Center)

[0031] 2. Experimental method

[0032] 2.1 Fungi Preparation

[0033] Tobacco mold was seeded on sandbar culture medium by scratching method, and the incubator was cultured for 2-3 days at 28 ° C. 5 ml of sterile PBS was placed in a petri dish, scraping the femoralira and scratch the cycles and spores, collecting mixed liquid, sterile gauze filtrate removal mycelium. At 4 ° C, 4500 rpm was centrifuged for 10 minutes, discarded, washed, and 5 ml of sterile PBS was added to the precipitate to form a spore suspension. Counting the blood cell count plate and adjusts the concentration of the spore to 5 × 10 with sterile PBS 4 Splitospor ...

Embodiment 2

[0039] Example 2 蟛蜞 Juku - Influence of Corneal Clinical Score, Pathological Change, Caspase-1 Signal, Interleukin 1β (IL-1β) secretion in fungal keratitis model mouse The role of treatment of fungal keratitis model mice.

[0040] Experimental material

[0041] 1.1 Experimental Pharmaceuticals: 蟛蜞蟛蜞 内 (Purchased from Selleck Company), Naomycin Drops (purchased from AlCon)

[0042] 1.2 Experimental Animals: C57BL / 6 Mice (Jiangsu Changzhou K Wers Experimental Animal Co., Ltd.)

[0043] 1.3 Experimental Fungi: 3.0,772 Tobacco Mildew (China Ordinary Microbial Square Savings Center)

[0044] 2. Experimental method

[0045] 2.1 model mouse

[0046] 8 weeks old health cleaning grade female C57BL / 6 mice systemic condition, experimental preparation crack lamp microscope examination excludes eye disease, random choice is experimentally. All animals are in line with the humanitarian treatments of the Chinese Science and Technology Experimental animals (VGKFCZ-2006-398) and meet the Ameri...

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Abstract

The invention belongs to the technical field of pharmacy, and discloses the application of wedelolide in the preparation of medicine for treating fungal keratitis. The present invention proposes for the first time that wedelolide has a therapeutic effect on fungal keratitis under in vivo and in vitro conditions. Under in vitro conditions, the human macrophage model stimulated by Aspergillus fumigatus was used to study the effect of wedelolide on the secretion of IL‑1β; Effects of esters on corneal clinical scores, pathological changes, Caspase-1 signaling pathway, and interleukin-1β secretion in model mice. In addition, the present invention also proposes the application of wedelia lactone combined with natamycin in the preparation of medicaments for treating fungal keratitis.

Description

Technical field [0001] The present invention is a pharmaceutical field, and more particularly to the use of a drug in the preparation of a drug for the treatment of fungal keratitis. Background technique [0002] Fungal Keratitis (FK) is a hyperlighted infectious corneal disease caused by fungi, and patients are mainly farmers in developing countries. In recent years, due to the excessive application of hormones and antibiotics, the increase in corneal traumatic contact mirror and the chance of corneal trauma have risen to the first place in infectious corneal disease, and patients are working in front. Young and middle-aged labor force. The treatment principle of fungal keratitis is early drug treatment, and drugs cannot control the disease in the final need for surgery. Since the conscious symptoms of the initial period of fungal corneal inflammation are not obvious, the patient has often taken a more severe corneal ulcer in the first time, and the common antifungal drug is nar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/37A61P27/02A61P31/10
Inventor 车成业张倩
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV