Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction

A technology of fingerprints and determination methods, which is applied in the field of determination of fingerprints of traditional Chinese medicine Shengxian Decoction, to achieve the effects of ensuring stability, good peak shape, and high technical content

Active Publication Date: 2021-05-04
合肥创新医药技术有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to control the quality of the traditional Chinese medicine compound Shengxian Decoction, analyze the types and content distribution information of each component in the traditional Chinese medicine Shengxian Decoction, the effective components of traditional Chinese medicine, the mechanism of action of traditional Chinese medicine, etc., it is necessary to establish a corresponding fingerprint control method, and in the existing In the technology, there is no fast, simple and accurate analysis method that can be used for the detection of the number, relative position, feature identification and content distribution of various chemical components of traditional Chinese medicine Shengxian Decoction.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction
  • A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction
  • A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction adopts high performance liquid chromatography, and its chromatographic conditions include: the chromatographic column is an octadecylsilane bonded silica gel chromatographic column (250×4.6mm, 5 μm), and acetonitrile is used as the mobile phase A, using phosphate buffered aqueous solution as mobile phase B, wherein the volume fraction of phosphoric acid is 0.1%, the concentration of potassium dihydrogen phosphate is 0.02mol / L, the detector is a DAD detector, the detection wavelength is 210nm, and the flow rate is 1.0ml / min, the column temperature is 35°C, and gradient elution is carried out;

[0068] The gradient elution program is: within 0-15min, the volume ratio of mobile phase A and mobile phase B changes gradually from 5:95 to 8:92 at a uniform speed; within 15-25min, the volume ratio of mobile phase A and mobile phase B changes from From 8:92 to 13:87 at a constant speed;...

Embodiment 2

[0087] A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction adopts high performance liquid chromatography, and its chromatographic conditions include: the chromatographic column is an octadecylsilane bonded silica gel chromatographic column (250×4.6mm, 5 μm), and acetonitrile is used as the mobile phase A, with the volume fraction being 0.2% phosphoric acid aqueous solution as mobile phase B, the detector is a DAD detector, the detection wavelength is 210nm, the flow velocity is 1.0ml / min, and the column temperature is 35°C, and gradient elution is carried out; the gradient elution Take off process with embodiment 1.

[0088] Solution preparation and test operation are the same as in Example 1.

[0089] For a typical spectrum see Figure 7 , Figure 7 It is the fingerprint spectrum of Shengchen Decoction measured in Example 2 of the present invention.

Embodiment 3

[0091] A method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction adopts high performance liquid chromatography, and its chromatographic conditions include: the chromatographic column is an octadecylsilane bonded silica gel chromatographic column (250×4.6mm, 5 μm), and acetonitrile is used as the mobile phase A, with a volume fraction of 0.1% phosphoric acid and a volume fraction of 1% tetrahydrofuran mixed aqueous solution as the mobile phase B, the detector is a DAD detector, the detection wavelength is 210nm, the flow rate is 1.0ml / min, and the column temperature is 35°C. Gradient elution is carried out; the gradient elution process is the same as in Example 1.

[0092] Solution preparation and test operation are the same as in Example 1.

[0093] For a typical spectrum see Figure 8 , Figure 8 It is the fingerprint spectrum of Shengchen Decoction measured in Example 3 of the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for determining the fingerprint of traditional Chinese medicine Shengxian Decoction, which adopts high-performance liquid chromatography, and the chromatographic conditions include: the chromatographic column is an octadecylsilane-bonded silica gel chromatographic column, and acetonitrile or methanol is used as the mobile phase A , with phosphate buffered saline solution as mobile phase B and DAD detector as the detector, gradient elution was carried out. The invention is fast, simple, comprehensive, accurate and reliable, and is suitable for detecting the fingerprint spectrum of Shengxiantang.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for determining fingerprints of traditional Chinese medicine Shengxian Decoction. Background technique [0002] The prescription of "Shengxian Decoction" comes from "Medical Zhongzhong Shenxilu" written by Zhang Xichun in the late Qing Dynasty, "to treat the depression of the air in the chest, shortness of breath and not enough to breathe...". [0003] The prescription composition of "Shengxian Decoction": raw astragalus 6 qian, Anemarrhena 3 qian, Bupleurum 1.5 fen, platycodon grandiflorum 1.5 fen, cohosh 1 qian. [0004] "Shengxian Decoction" Functions and Indications: To cure the depression of the air in the chest, shortness of breath, or breathing hard, it seems to be panting, or the breath will stop, and the danger is in an instant. Also see alternating cold and heat, or dry throat and thirst, or full of depression, or dizziness and forgetfuln...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/86G01N30/02G01N30/34G01N30/74G01N30/06G01N30/14
Inventor 曹明成董宠曹阳
Owner 合肥创新医药技术有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com