Tissue culture propagation method for anthurium andreanum

A technology of anthurium group and anthurium, which is applied in the field of anthurium tissue culture and propagation, can solve the problems of high price and achieve the effects of low cost, shortened tissue culture time, and fast rooting

Inactive Publication Date: 2019-06-04
广西北流森艺瓷业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is loved by the majority of people, but its core cultivation technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Select Anthurium as explants, wash the explants with pure water for 2-3 times, then soak in 0.1% zinc blue powder solution with volume concentration for 30min, wash with clean water and rinse with 0.15% HgCl 2 Soak the explants in the solution for 15-20 minutes to sterilize the explants, and finally rinse the explants with sterile water, place them on sterile filter paper to absorb the water, and set aside.

[0021] Inoculate the stem segment of the treated Anthurium strain into the induction medium for culture, first culture in the dark for 3-4 days, and then culture in the light, the light time is 12h / d, the light intensity is 500lx, the culture temperature is 22-25°C, and the culture time is 20 -22d, Anthurium callus was cultured, and the callus induction rate reached 99%; wherein, the formula of the induction medium was: improved LS medium+6-BA6.0 mg / L+IBA 2.0 mg / L L +VC 15 mg / L +polyvinylpyrrolidone 50mg / L+VB 2 15 mg / +agar powder 3.6g / L+sucrose 20 g / L.

[0022] ...

Embodiment 2

[0026]Select Anthurium as explants, wash the explants with pure water for 2-3 times, then soak in 0.1% zinc blue powder solution for 35 minutes, wash with clean water and rinse with 0.15% HgCl 2 Soak the explants in the solution for 15-20 minutes to sterilize the explants, and finally rinse the explants with sterile water, place them on sterile filter paper to absorb the water, and set aside.

[0027] Inoculate the stems of the treated Anthurium strains into the induction medium for culture, first cultivate in the dark for 3-4 days, and then cultivate in the light, the light time is 12h / d, the light intensity is 1000lx, the culture temperature is 25-26°C, and the culture time is 20 -22d, Anthurium callus was cultured, and the callus induction rate reached 99%; wherein, the formula of the induction medium was: improved LS medium+6-BA7.5 mg / L+IBA 2.5 mg / L L +VC 15 mg / L +polyvinylpyrrolidone 50mg / L+VB 2 15 mg / +agar powder 3.6g / L+sucrose 20 g / L.

[0028] The obtained Anthurium ...

Embodiment 3

[0032] Select Anthurium as explants, wash the explants with pure water for 2-3 times, then soak in 0.1% zinc blue powder solution with volume concentration for 40min, wash with clean water and rinse with 0.15% HgCl 2 Soak the explants in the solution for 15-20 minutes to sterilize the explants, and finally rinse the explants with sterile water, place them on sterile filter paper to absorb the water, and set aside.

[0033] Inoculate the stems of the treated Anthurium strains into the induction medium for culture, first culture in the dark for 3-4 days, and then culture in the light, the light time is 12h / d, the light intensity is 1000lx, the culture temperature is 26-28°C, and the culture time is 20 -22d, Anthurium callus was cultured, and the callus induction rate reached 99%; wherein, the formula of the induction medium was: improved LS medium+6-BA8.0 mg / L+IBA 3.0 mg / L L +VC 15 mg / L +polyvinylpyrrolidone 50mg / L+VB 2 15 mg / +agar powder 3.6g / L+sucrose 20 g / L.

[0034] The o...

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PUM

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Abstract

The invention discloses a tissue culture propagation method for anthurium andreanum. The method includes explant treatment, induction culturing, proliferation culturing and rooting culturing; daughterplants of anthurium andreanum are selected as explants; the explants are inoculated into an induction medium to perform cultivation after being washed and treated through sterilization and disinfection, so that anthurium andreanum calluses can be obtained; then, the anthurium andreanum calluses are transferred to a proliferation medium to perform proliferation culturing; and differentiated multiple shoots with lengths of 1-2 cm are transferred to a rooting medium to perform cultivation, so that rooting anthurium andreanum can be obtained, and rooting rates can reach 100%. Through the induction, proliferation and rooting on the shoot tips of the anthurium andreanum, anthurium andreanum tissue culture seedlings can be rapidly obtained; the method is fast in rooting, so that the tissue culture time of the anthurium andreanum can be shortened, and therefore, low costs can be achieved; and the survival, differentiation and rooting rates of the anthurium andreanum can be enhanced, so that the method can have good economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, and relates to an anthurium tissue culture propagation method. Background technique [0002] Anthurium, also known as anthurium, anthurium, is a perennial epiphytic evergreen herb of the family Araceae Anthurium, native to the tropical rain forests of South America. Its flowers are unique, with Buddha flame inflorescences, bright and gorgeous, rich in color, each flower has a long flowering period, and the color of the flower changes greatly. Common bract colors are red, pink, white, etc., which have great ornamental value. In recent years, the quality of anthurium has been getting higher and higher, the domestic market is relatively stable, the market acceptance is getting better and better, the sales are stable, and the prospect is promising. Since 1980, tissue culture technology has developed rapidly. Since the introduction of anthurium in my country in the 1980s, due to its unique...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈向阳李海飞
Owner 广西北流森艺瓷业有限公司
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