Micro-fluidic chip detecting system and method for sorting and focusing of integrated cells

Abstract

The invention discloses a micro-fluidic chip detecting system and method for sorting and focusing of integrated cells. The micro-fluidic chip detecting system comprises a viscoelastic fluid sampling device, a sample sampling device, a waste liquid collecting device, a high-speed image acquiring device, a micro-fluidic chip, a sample collecting device and a microcomputer, wherein the micro-fluidicchip is manufactured by encapsulating of a flow channel layer and a basal layer; and a spiral flow channel, a branch flow channel, a lower division flow channel, an upper division flow channel, a viscoelastic fluid sampling flow channel, a spiral mixing flow channel and a focusing main flow channel are arranged in the flow channel layer. Through the adoption of the micro-fluidic chip detecting system disclosed by the invention, cells are sorted according to the dimension characteristics, the viscoelastic bearing fluid is mixed and blended, and the sorted rare cells are focused in single equilibrium positions at the centers of the sections of the flow channels, so that the accuracy of counting and detection is improved.

Description

technical field [0001] The present invention relates to a biological particle control and detection technology based on a microfluidic chip, in particular to a microfluidic control chip integrating spiral channel inertial sorting technology, spiral channel mixing technology and viscoelastic inertial direct current channel focusing technology. Cell count detection system. Background technique [0002] There are rare but clinically important rare cells in body fluids such as blood, urine, and pleural effusion. However, because the content of rare cells is very low, counting and detecting them is facing great challenges. At present, rare cell detection techniques commonly used in clinical laboratories mainly include histochemical staining, immunohistochemistry, and flow cytometry. Expensive and other defects. [0003] Microfluidic technology can achieve precise control of microfluidics, and has the advantages of precise control, high efficiency and low cost, and is widely use...

AI Technical Summary

Problems solved by technology

However, after the sorted cells enter the new flow channel, the original focusing state of the cells may be affected, thereby affecting the detection accuracy; and when the cells are counted and detected, it is difficult for the cells to maintain a single focus, and cells will appear side by side, multiple Several cells pass through the counting detection area at the same time, which reduces the accuracy of cell counting detection

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