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Method for extracting and separating effective components of rehmannia glutinosa Libosch and extracted catalpol and polysaccharides

A technology of an active ingredient and a separation method, which is applied to the extraction and separation method of Rehmannia glutinosa and the field of catalpol and polysaccharide extracted, can solve the problems of high extraction cost, waste of ingredients, low yield and the like, and achieves that the enzymatic hydrolysis yield is improved and the utilization rate is improved. efficiency, easy operation

Inactive Publication Date: 2019-06-07
北京国康本草物种生物科学技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The yield rate of the above several methods is low, and other effective medicinal ingredients in Rehmannia glutinosa are wasted seriously, and the extraction cost is high

Method used

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  • Method for extracting and separating effective components of rehmannia glutinosa Libosch and extracted catalpol and polysaccharides
  • Method for extracting and separating effective components of rehmannia glutinosa Libosch and extracted catalpol and polysaccharides
  • Method for extracting and separating effective components of rehmannia glutinosa Libosch and extracted catalpol and polysaccharides

Examples

Experimental program
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Effect test

Embodiment 1

[0054] The method for extracting and separating the effective components of Rehmannia glutinosa provided in this embodiment specifically includes the following steps:

[0055] 1) Take fresh Rehmannia glutinosa and chop its stems and leaves, add a mixture of pectinase and cellulase with 0.01% of the mass of Rehmannia glutinosa, add water 5 times the mass of Rehmannia glutinosa, control the temperature at 30°C, and perform enzymatic hydrolysis for 48 hours.

[0056] 2) Add 2.5 times the weight of Rehmannia glutinosa, extract at 30°C for 30 minutes, filter, add 5 times of 70% ethanol to the filter cake for 30 minutes, filter, continue to add 5 times of 90% ethanol to the filter cake for 30 minutes, filter, add 5 times of 90% ethanol to the filter cake, filter, add 5 times 90°C pure water extraction for 30min. Filtration to obtain filter residue and filtrate, the filter residue can be used as plant fertilizer after drying, and the extracts of all steps are mixed to obtain the fina...

Embodiment 2

[0061] The method for extracting and separating the effective components of Rehmannia glutinosa provided in this embodiment specifically includes the following steps:

[0062] 1) Chopped fresh rehmannia root and its stems and leaves, added pectinase, cellulase and hemicellulase with 0.1% of the mass of rehmannia glutinosa, added water 10 times the mass of rehmannia glutinosa, controlled the temperature at 50°C, and performed enzymatic hydrolysis for 24 hours.

[0063] 2) Add 5 times the weight of Rehmannia glutinosa, extract at 50°C for 60 minutes, filter, add 10 times of 70% ethanol to the filter cake for 60 minutes, filter, add 10 times of 90% ethanol to the filter cake for 60 minutes, filter, add 10 times of 90% ethanol to the filter cake, filter, add 10 times 100°C pure water extraction for 60min. Filtration to obtain filter residue and filtrate, the filter residue can be used as plant fertilizer after drying, and the extracts of all steps are mixed to obtain the final ext...

Embodiment 3

[0068] The method for extracting and separating the effective components of Rehmannia glutinosa provided in this embodiment specifically includes the following steps:

[0069] 1) Take fresh Rehmannia glutinosa and its stems and leaves, chop it up, add pectinase with 0.05% of the mass of Rehmannia glutinosa, add water 7 times the mass of Rehmannia glutinosa, control the temperature at 40°C, and perform enzymatic hydrolysis for 36 hours.

[0070] 2) Add 3 times the weight of Rehmannia glutinosa, extract at 40°C for 40 minutes, filter, add 7 times 70% ethanol to the filter cake for 40 minutes, filter, add 7 times 90% ethanol to the filter cake for 40 minutes, filter, add 70% ethanol to the filter cake 7 times pure water at 95°C, extract for 4 minutes. Filtration to obtain filter residue and filtrate, the filter residue can be used as plant fertilizer after drying, and the extracts of all steps are mixed to obtain the final extract.

[0071] 3) Concentrate the final extract to re...

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Abstract

The invention provides a method for extracting and separating effective components of rehmannia glutinosa Libosch and extracted catalpol and polysaccharides. A plurality of components of the rehmanniaglutinosa Libosch are extracted, which gets rid of the limitation of extraction and separation of a single target, greatly improves the utilization rate of the rehmannia glutinosa Libosch and finallyachieves multi-target co-production of the catalpol and the polysaccharides. The method is simple in operation and low in equipment requirements, improves the yield by 3-5 times by using enzymatic hydrolysis, saves important raw materials, and is easy in industrial mass production. The extraction and separation method obtains the catalpol monomer and the polysaccharide monomer, wherein the purityof the catalpol monomer is greater than or equal to 98%.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for extracting and separating effective components of Rehmannia glutinosa and extracted catalpol and polysaccharide. Background technique [0002] Rehmannia glutinosa Libosch is a plant of the Scrophulariaceae Rehmannia genus, also known as Dimeng, Baba, etc. The roots are used as medicine and artificially cultivated. The large-scale planting areas are distributed in Yuncheng, Linfen, Shanxi, Jiaozuo, Henan, and Weinan, Shaanxi. It is also planted in Beijing, Hebei, Shandong, and Gansu. Due to the different processing methods, the medicinal materials are divided into fresh rehmannia, raw rehmannia and rehmannia glutinosa, which are typical of the changes in the properties of traditional Chinese medicine after processing. Fresh rehmannia mainly treats injuries, expels blood stagnation, fills bone marrow, and grows muscles. The chemical composition of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08C08B37/00
Inventor 王代军陈松
Owner 北京国康本草物种生物科学技术研究院有限公司
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