In-vitro leaf section identification method for resistance of wheat to stripe rust

Abstract

The invention discloses an in-vitro leaf section identification method for resistance of wheat to stripe rust. The method is as below: 8-12 days after wheat seeds to be detected are sown, when first leaves of wheat seedlings are completely unfolded and core leaves are just exposed, the first leaves of the wheat seedlings are cut into leaf sections with the length of 2-3 cm on an ultra-clean workbench, and the front faces of the leaves are flatly laid on the surface of a 0.5% water agar culture medium containing 60-80 mg / L benzimidazole upwards; the fresh summer spores of the puccinia striiformis and 0.05% Tween 20 aqueous solution are uniformly mixed, the cut leaf sections are sprayed and inoculated with the mixture; the inoculated wheat leaf segments are transferred into an illumination incubator, moisture is preserved for 24 hours under the conditions that the temperature is dark and the humidity is 100%, and then culturing is conducted for 14-16 days under the conditions that the illumination intensity is 6000 Lux-8000 Lux and the relative humidity is 60%-80%; and the reaction types of the wheat stripe rust are distinguished according to 0-4 levels of standards. Compared with the prior art, the wheat stripe rust resistance condition can be quickly identified according to the wheat stripe rust reaction types by inoculating and culturing the wheat in-vitro leaves with the wheat stripe rust, time and labor are saved, the influence of environmental conditions is avoided, experimental materials are saved, the space is saved, and the method is simple, convenient and quick.

Description

technical field [0001] The invention relates to the technical field of wheat stripe rust resistance detection, in particular to a method for identifying isolated leaf segments of wheat stripe rust resistance. Background technique [0002] Stripe rust is one of the most important diseases of wheat, which occurs all over the world, especially in my country. Wheat stripe rust (Puccnia striiformis f.sp.tritici), which causes wheat stripe rust, is a typical obligate parasite, which can only survive on living organisms and cannot be cultivated artificially. Therefore, at present, the monitoring of the physiological races of wheat stripe rust and the identification of wheat stripe rust resistance can only be carried out by cultivating healthy wheat plants in greenhouses and fields, through artificial inoculation or induced inoculation, moisturizing, light cultivation, etc., to make wheat plants disease. This method is not only time-consuming and labor-intensive, but also requires ...

AI Technical Summary

Problems solved by technology

[0004] However, at present, the monitoring of the physiological races of wheat stripe rust and the identification of wheat stripe rust resistance can only be carried out by cultivating healthy wheat plants in the greenhouse, then artificially inoculating them by smearing or dusting, and after moisturizing and low-temperature incubation. disease on wheat material

This identification method often needs to occupy a large space, not only time-consuming and labor-intensive, but also requires a large amount of bacteria and wheat materials, and the identification results are easily affected by environmental conditions.

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