Micrornas as biomarkers for endometriosis

A technology for endometriosis and heterotopia, which is used in the determination/inspection of microorganisms, biological testing, biological material analysis, etc.

Pending Publication Date: 2019-06-14
YALE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, for a definitive diagnosis of endometriosis, direct visualization and histological confirmation of the lesion through surgical procedures is required, which requires general anesthesia, skilled surgical technique, surgical cost, and risk of potential complications

Method used

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  • Micrornas as biomarkers for endometriosis
  • Micrornas as biomarkers for endometriosis
  • Micrornas as biomarkers for endometriosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0222] Example 1: Serum microRNAs as diagnostic markers for endometriosis; comprehensive array-based analysis

[0223]Microarray studies of ectopic and ectopic endometrial tissue from women with and without endometriosis revealed differential expression of some miRNAs (Ohlsson et al., 2009, Mol Endocrinol, 23:265- 275, Petracco et al., 2011, J Clin Endocrinol Metab, 96:E1925-1933). They play an important role in the pathogenesis of endometriosis and related infertility by regulating gene expression (Teague et al., 2010, HumReprod Update, 16:142-165). Tissue miRNAs are shed from pathological tissues into the circulation, and a strong correlation has been shown between circulation and tissue levels (Resnick et al., 2009, Gynecol Oncol, 112:55-59). Some differentially expressed miRNAs have been identified in the serum of patients with endometriosis (jia et al., 2013, Hum Reprod, 28:322-330, Wang et al., 2013, J Clin Endocrinol Metab, 98: 281 -289). It has previously been sho...

Embodiment 2

[0256] Example 2: Relative expression of miRNAs in endometriotic lesions in baboons

[0257] Such as Figure 4 As shown, the relative expression of miRNAs in endometriotic lesions in baboons was determined by real-time quantitative PCR. The relative expression levels of miR-125b-5p, miR-150-5p and miR-3613-5p were compared to the relative expression levels of the respective miRNAs after statin treatment. Lesion shrinkage was observed in statin treated animals. Expression levels of miR-125b-5p in samples from untreated animals were shown to have higher relative expression levels than samples taken from treated animals. Furthermore, it was observed that the relative level of miR-3613-5p was lower in samples from control untreated animals compared to the level of miR-3613-5p in samples from treated animals. Finally, it was observed that the relative level of miR-150-5p was lower in samples from control untreated animals compared to the level of miR-150-5p in samples from tre...

Embodiment 3

[0258] Example 3: Salivary microRNA as a diagnostic marker for endometriosis

[0259] Step 1: Extraction of RNA from saliva

[0260]Saliva samples (200 μL) were collected and transferred to 1.5 mL tubes. RNase-free water was added to the samples in volumes less than 200 μL to bring the total sample volume to 200 μL. 1 mL of QIAzol Lysis Reagent (Qiagen) was added to the sample. The tubes were vortexed briefly and the samples were incubated at room temperature for five minutes. Then, 200 μL of chloroform was added to the lysate and vortexed for about 15 seconds. The sample mixture was then incubated at room temperature for 2 minutes and centrifuged at 12,000 xg for 15 minutes in a cryogenic chamber (approximately 4°C). Transfer approximately 560 µL of the aqueous phase to a new 1.5 mL tube. Add 840 μL of 100% ethanol to 560 μL of the aqueous phase to obtain a total volume of 1400 μL. Then, transfer 700 μL of the mixture to an RNeasyMiniElute spin column with a 2 mL co...

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Abstract

Disclosed herein are compositions and methods useful for the diagnosis, assessment, and characterization of endometriosis in a subject in need thereof, based upon the expression level of at least onemiRNA that is associated with endometriosis.

Description

[0001] References to related applications [0002] This patent application claims priority to U.S. Provisional Patent Application Serial No. 62 / 381,130, filed August 30, 2016, which is incorporated herein by reference in its entirety. Background technique [0003] MicroRNAs (miRNAs) are a class of highly conserved small endogenous noncoding, functional RNA molecules of 19-24 nucleotides; they control which RNAs they target through base pairing with complementary sites translation and stability of messenger RNA transcripts and cause repression or degradation of messenger RNA transcripts (Bartel et al., 2009, Cell, 136:215-233). They play key roles in the regulation of development and cellular homeostasis of a variety of biological processes (Hassan et al., 2015, Bone, 81:746-756). They are present intracellularly as well as in serum (Corcoran et al., 2011, Clin Chem, 57(1):18-32) and have specific features that make them promising biomarker candidates for the diagnosis of vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105C12Q1/6883G01N33/50C12N15/11
CPCG01N33/5308G01N33/689G01N2800/364C12Q1/6883C12Q2600/178A61P15/00C12Q2600/158C12Q2600/106C12Q2600/112
Inventor 休·泰勒
Owner YALE UNIV
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