A high-throughput, high-content drug screening microfluidic chip and its preparation method
A microfluidic chip, high-throughput technology, applied in the direction of chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve the problems of high cost, cumbersome operation process, complex optical path, etc., to reduce research and development costs , Improve the effect of efficiency and precision
Active Publication Date: 2021-06-11
TAIYUAN UNIV OF TECH
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Problems solved by technology
[0005] Generally, high-throughput and high-content drug screening is usually completed on two independent chips, which makes the drug screening process extremely cumbersome and the screening cost is relatively high. Some researchers have tried to integrate SPR and SERS together. , but its optical path is very complicated and the cost is high; therefore, realizing the functional integration of low-cost high-throughput and high-content drug screening technology has important theoretical and practical significance for promoting the development of innovative drugs
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[0070] like figure 1 As shown, it is a structural diagram of a drug screening microfluidic chip combined with electricity and optics. There are two different microelectrode arrays on this type of chip, one is a three-electrode array with an organic semiconductor film in the middle of the electrode, and the other is a three-electrode array. One is a two-electrode array containing noble metal dendrites in the middle of the electrodes; the electrode arrays are covered with a plurality of microchannels, and a plurality of pneumatic microvalves are arranged on the microchannels, and a row of microvalves are respectively arranged on the top and bottom of the chip Rectangular pads for leading out microelectrodes.
[0071] The microfluidic chip provided by the present invention mainly includes a substrate and a cover sheet, the rectangular pads and electrode arrays are located on the substrate, and the microfluidic channels and microvalves are located on the cover sheet.
[0072] lik...
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The invention belongs to the technical field of microfluidic chips, and specifically relates to a high-throughput, high-content drug screening microfluidic chip and a preparation method thereof; the technical problem to be solved is: to provide a high-throughput, high-content drug screening chip Microfluidic chip and its preparation method; the technical solution adopted to solve this technical problem is: the chip includes a cover sheet, the cover sheet is arranged on the substrate, and a plurality of microfluidic channels are arranged on the cover sheet, and the inside of each microfluidic channel Both are provided with a fluid transport channel and a microvalve gas control channel; the fluid transport channel includes a liquid inlet and a liquid outlet, the liquid inlet is evenly distributed laterally at one end of the cover, and the liquid outlet is evenly distributed laterally at the other end of the cover One end; the liquid inlet is connected to the input main channel, and the output end of the input main channel is divided into a plurality of input branch channels, and each input branch channel is further divided into two luan channels, and each luan channel passes through a miniature round pool; the application of the present invention Preparation of microfluidic chips for drug screening.
Description
technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a high-flux, high-content drug screening microfluidic chip and a preparation method thereof. Background technique [0002] In the past 10 years, on average, only 30 drugs have passed the new drug approval of the US Center for Drug Evaluation each year, and about 500 targets have been obtained from drug screening, accounting for only 5%-10% of the predicted results of the Human Genome Project. Serious challenges; [0003] High-throughput molecular-level drug screening relies on microfluidic chip technology with low reagent consumption and high detection sensitivity, often combined with surface plasmon resonance (SPR) technology as a detection method. [0004] The latest high-content drug screening detection can be realized through label-free technology. Using surface-enhanced Raman spectroscopy (SERS) technology, researchers have realized the type analysis ...
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IPC IPC(8): B01L3/00
Inventor 冀健龙付银鹏王靖宵江小宁桑胜波张文栋
Owner TAIYUAN UNIV OF TECH