Bacillus megatherium for promoting saline-alkaline-resistant growth of crops, special microbial fertilizer for saline-alkali land and application of bacillus megatherium in special microbial fertilizer

A technology of Bacillus megaterium and microbial fertilizer, which is applied to a strain of Bacillus megaterium that promotes the growth of crops resistant to salt and alkali, and special microbial fertilizers for saline-alkali land, can solve the problems of low crop yield and difficult land use, and improve utilization efficiency , soil improvement, low cost of raw materials

Active Publication Date: 2019-06-18
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] Another object of the present invention is to solve the practical problems of low yield of crops in saline-alkali land and difficult use of land in agricultural production, to develop a special functional microbial organic fertilizer for saline-alkali land, which can

Method used

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  • Bacillus megatherium for promoting saline-alkaline-resistant growth of crops, special microbial fertilizer for saline-alkali land and application of bacillus megatherium in special microbial fertilizer
  • Bacillus megatherium for promoting saline-alkaline-resistant growth of crops, special microbial fertilizer for saline-alkali land and application of bacillus megatherium in special microbial fertilizer
  • Bacillus megatherium for promoting saline-alkaline-resistant growth of crops, special microbial fertilizer for saline-alkali land and application of bacillus megatherium in special microbial fertilizer

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Embodiment Construction

[0040] 1. Soil sample collection and Bacillus isolation and screening

[0041] Saline-alkali soils from all over the country were collected and potted experiments were carried out. On these soils, the variety of Jingtian Zihuanuo was planted, watered normally, and the corn plants were harvested one month later. The soil suspension of rhizosphere soil was extracted separately, diluted and spread on LB plates after 90 °C water bath for 15 minutes, and cultured overnight at 37 °C. The bacteria on the plates were isolated and purified respectively, and a total of 124 strains of Bacillus were screened out, and the glycerol tubes were stored in -80°C refrigerator.

[0042] 2. Screening of strains to improve crop salt tolerance

[0043] Disinfect the surface of corn seeds and place them in a sterilized petri dish, place sterile filter paper moistened with sterile water at the bottom of the petri dish, and keep them in the dark for 3 days in a refrigerator at 4°C for vernalization; p...

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Abstract

The invention discloses a strain of bacillus megatherium for promoting the saline-alkaline-resistant growth of crops, a special microbial fertilizer for a saline-alkali land and application of the bacillus megatherium in the special microbial fertilizer. The strain T4-9 belongs to Bacillus Megatherium (the patent preservation number of the strain is CGMCC17334). Tests show that after the strain T4-9 microbial fertilizer is applied to saline-alkali soil of different degrees, the salt-resistant stress capacity of the crops can be improved, the yield of the crops is increased, the salt content ofsoil can be reduced, and the physicochemical property of the soil is improved. The application of the strain T4-9 microbial fertilizer in the saline-alkali land can relieve the damage of salt stressto the crops, increase the yield of the crops in the saline-alkali land, and provide a feasible scheme for the improvement and agricultural utilization of the saline-alkali land.

Description

technical field [0001] The invention belongs to the agricultural intensive production technology, and specifically relates to a plant rhizosphere growth-promoting bacterium T4-9 and its application. The saline-alkali soil special microorganism with the plant rhizosphere growth-promoting bacterium T4-9 that promotes the growth of corn saline-alkali resistance is applied in saline-alkali land Fertilizer can increase the yield of corn, reduce the salt content of saline-alkali soil, improve the physical and chemical properties of saline-alkali soil, and realize the agricultural utilization of saline-alkali land. Background technique [0002] Soil salinization is a global environmental problem. The total area of ​​saline-alkali land in my country is 99.13 million hectares, accounting for about 10% of the total area of ​​saline-alkali land in the world. Among them, coastal saline-alkali land is widely distributed, involving the coastlines of 11 provinces (regions) and cities in Ch...

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C05F11/08C12R1/11C05G3/80
CPCY02W30/40
Inventor 张瑞福陈小娟徐志辉荀卫兵邵佳慧张楠冯海超
Owner NANJING AGRICULTURAL UNIVERSITY
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