Gamma-aminobutyric acid (GABA) enrichment method based on mixotrophic haematococcus pluvialis

A technology of Haematococcus pluvialis and GABA, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as high cost, food safety problems, low content and yield, and achieve Simple operation, shorten the growth cycle, and improve the effect of biomass

Active Publication Date: 2019-06-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is costly and has certain food safety problems
The enrichment of γ-GABA in plants generally uses chemical inducers to induce plants to accumulate γ-GABA, but its content and yield are still low.

Method used

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  • Gamma-aminobutyric acid (GABA) enrichment method based on mixotrophic haematococcus pluvialis
  • Gamma-aminobutyric acid (GABA) enrichment method based on mixotrophic haematococcus pluvialis

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preparation example Construction

[0027] Step 1, preparation of induced algae liquid: at a temperature of 24-26°C and a light of 2800-3500lx, the basic BBM medium with 1.5-2.5g / L sodium acetate as a carbon source is also used to culture Haematococcus pluvialis, and wait for the rain Haematococcus grows to the late logarithmic phase to collect the algal cells, dilute and resuspend the algal cells with fresh BBM medium as the induced algal liquid, and the concentration of the algal liquid is 0.1-0.5g / L;

[0028] Step 2. Induce algal cells to accumulate γ-GABA: prepare 1g / L NaCl mother liquor with pure water, add appropriate amount of NaCl mother liquor to the induced algae liquor so that the NaCl concentration is 0.2-0.6g / L, and place it at 26°C-28 ℃, light intensity 12000-14000lx, cold light continuous light induction;

[0029]Step 3. Collect the algae cells the next day and detect the γ-GABA content: collect the algae liquid the next day, first enrich the algae liquid by centrifugation at 3500r / min for 10 minu...

Embodiment 1

[0031] This embodiment is based on the method for producing gamma-aminobutyric acid from Haematococcus pluvialis, the main steps of which are:

[0032] (1) When the temperature is 24°C and the light is 2800lx, the basic BBM medium with 1.5g / L sodium acetate as the carbon source is also cultured for Haematococcus pluvialis, and collected after the growth of Haematococcus pluvialis to the late logarithmic growth phase For algae cells, dilute and resuspend the algae cells with fresh BBM medium as the induced algae liquid, and the concentration of the algae liquid is 0.1g / L.

[0033] (2) Prepare 1g / L NaCl mother liquor with pure water, add an appropriate amount of NaCl mother liquor to the induced algae liquor to make the NaCl concentration 0.2g / L, and place it at 26°C under a light intensity of 12000 lx for induction culture;

[0034] (3) The algae cells were collected by centrifugation the next day, and the γ-GABA in the algae cells was extracted by using an organic solvent, and...

Embodiment 2

[0037] This embodiment is based on the method for producing gamma-aminobutyric acid from Haematococcus pluvialis, the main steps of which are:

[0038] (1) When the temperature is 25°C and the light is 3000lx, the basic BBM medium with 2g / L sodium acetate as the carbon source is used to culture Haematococcus pluvialis, and the algae are collected after the growth of Haematococcus pluvialis to the late logarithmic growth phase. Cells, dilute and resuspend algae cells with fresh BBM medium as the induced algae liquid, the concentration of the algae liquid is 0.3g / L.

[0039] (2) Prepare 1g / L NaCl mother liquor with pure water, add an appropriate amount of NaCl mother liquor to the induced algae liquor to make the NaCl concentration 0.4g / L, and place it at 27°C with a light intensity of 13000lx for induction culture;

[0040] (3) collect algae cells by centrifugation the next day, utilize organic solvent to extract gamma-GABA in the algae cells, and utilize liquid chromatography ...

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Abstract

The invention discloses a gamma-aminobutyric acid (GABA) enrichment method based on mixotrophic haematococcus pluvialis. The method mainly includes the steps that haematococcus pluvialis is cultured to the later period of a logarithmic culture period by using sodium acetate as an organic carbon source, and the cultured haematococcus pluvialis is used as an inducing culture solution after being diluted and resuspended by a fresh BBM culture medium; a 1 g / L NaCl mother solution is prepared through pure water, and then is added into a diluted inducing algae solution so as to be diluted, and the algae solution is cultured under high illumination; and the algae solution is collected next day, an organic solvent is used for extracting GABA in algae cells, and the content of the GABA is detected.According to the method, operation is easy and practicable, the algae cell growth period can be shortened, the biomass of the haematococcus pluvialis is increased, and the content of the GABA in thehaematococcus pluvialis is greatly increased.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for enriching gamma-aminobutyric acid based on Haematococcus pluvialis. Background technique [0002] Microalgae has attracted widespread attention due to its fast growth rate and vigorous vitality. Microalgae can be used to produce a variety of by-products, mainly including oil, protein, carbohydrates, natural pigments, antioxidants, amino acids, etc. γ-GABA has become the focus of extensive attention of scholars because of its anti-anxiety, anti-oxidation and reproductive physiological functions. However, at present, natural γ-GABA is basically extracted from plants. Compared with plants, microalgae have the advantage of a shorter growth cycle. Through detection, it is found that Haematococcus pluvialis also contains γ-GABA, but the content is not high. How to improve the γ-GABA content and γ-GABA yield of Haematococcus pluvialis is an urgent problem...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/89
Inventor 余旭亚赵永腾丁巍崔静
Owner KUNMING UNIV OF SCI & TECH
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