Supercharge Your Innovation With Domain-Expert AI Agents!

Improvement method of direct PCR trace gene identification technology

A direct, anchored technique applied in the field of direct PCR

Inactive Publication Date: 2019-06-18
中禾生物种业集团有限公司
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of direct PCR amplification in trace crude components

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Improvement method of direct PCR trace gene identification technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] 1 Assuming the processing of DNA from a microsample of corn as an example. The 10μg trace amount of DNA in corn also comes naturally from corn, with a small amount and a lot of impurities. The corn sample was firstly lysed with lysate for 2 minutes, and then digested with proteinase K for 2 minutes at the same time. The formula of the lysate is 50mM / L Tris, pH8, 25mM / L EDTA, 3% SDS, 12% PVP. According to our analysis, adding hydroxymethylcellulose sodium 0.5M / L containing cobalt chloride 1mM / L to the lysate for 2 minutes can significantly enhance the solution distribution efficiency of DNA and improve the polymerization of DNA polymerase in crude materials. role.

[0009] 2 Primer design online website Primer 3: http: / / bioinfo.ut.ee / primer3-0.4.0 / , browse and query, and test the specificity of the primer online through the specific test Blast primer tool. When designing primers, a specific PCR 3-primer method was used to amplify the maize genome. The design of speci...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an improved direct PCR method. 0.5 M / L of carboxymethyl cellulose sodium of 1 mM / L of cobalt chloride is added into a reaction system of heat-resisting DNA polymerase for treatment, during PCR amplification, an inner primer and an outer primer are designed for amplification of a target sequence, and the appropriate amplification conditions are groped as the reference. In this way, even if only a trace microgram-level coarse component genome DNA exists, effective direct amplification can also be obtained. The extraction process of the trace DNA is omitted, the working efficiency is improved, compared with a traditional PCR method, according to the improved direct PCR method, the amplification efficiency of the trace microgram-level coarse component genome DNA is greatly improved.

Description

technical field [0001] This experimental invention patent relates to a direct PCR technology, especially the direct PCR technology using the crude component trace genome. Background technique [0002] At present, PCR technology has developed into the mainstream of various identification technologies, and PCR technology has developed diversity, and has played an increasingly important role in all walks of life and various applications. In the identification process of crop germplasm resources, relying on traditional biochemical and genetic methods has been difficult to meet people's needs for variety identification. PCR technology can be used to identify various germplasm resources, and it has played an increasingly important role in modern molecular breeding. [0003] Among the various methods of identification, the identification of trace samples has attracted widespread public attention. In general, samples collected in the field are not like other samples. Planted samp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/686
Inventor 不公告发明人
Owner 中禾生物种业集团有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com