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A quick cleaning method for entomopathogenic nematodes

An entomopathogenic nematode, rapid technology, applied in chemical instruments and methods, wet separation, solid separation, etc., can solve the problems of long nematode extraction time, no involvement of entomopathogenic nematode infectivity and survival rate, and extraction rate to be improved, etc. , to achieve the effect of shortening the cleaning recovery time, shortening the cleaning time, and shortening the time used

Active Publication Date: 2021-04-13
KINGENTA ECOLOGICAL ENG GRP +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cleaning method of entomopathogenic nematodes in this invention adopts the method of static soaking, the extraction time of nematodes is longer, and the extraction rate needs to be improved, and relevant data such as the infectivity and survival rate of entomopathogenic nematodes are not involved.

Method used

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  • A quick cleaning method for entomopathogenic nematodes
  • A quick cleaning method for entomopathogenic nematodes

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A quick cleaning method for entomopathogenic nematodes, comprising the steps of:

[0034] (1) Cleaning: Put the culture mixture obtained above into the washing bucket of the washing machine (the water injection volume is 40L), and the volume ratio of the water injection volume in the washing machine to the culture mixture is 2:1; Washing 8min-dehydration 1min-rinsing 4min-dehydration 1min-rinsing 4min-dehydration 1min, the total cleaning time is 19min, and the washing machine discharge liquid is collected; the speed of the single washing is 40rpm, and the speed of the rinsing is 40rpm, The rotational speed of the dehydration is 300rpm; about 96% of the nematodes are in the discharge liquid.

[0035](2) Concentration and separation: the effluent obtained in step (1) is left to settle for 20 minutes in an inclined tank; the tank is a cuboid tank, and the aspect ratio in the tank is 2:1, and the angle between the tank and the horizontal plane is 30°; along the inclined di...

Embodiment 2

[0038] A quick cleaning method for entomopathogenic nematodes, comprising the steps of:

[0039] (1) Cleaning: Put the culture mixture obtained above into the washing bucket of the washing machine (the water injection volume is 40L), and the volume ratio of the water injection volume in the washing machine to the culture mixture is 3:1; Washing 4min-dehydration 2min-rinsing 3min-dehydration 2min, the total cleaning time is 11min, and the discharge liquid of the washing machine is collected; the speed of the single washing is 40rpm, the speed of the rinsing is 40rpm, and the speed of the dehydration is 40rpm. 500rpm; about 95% of the nematodes are in the discharge liquid.

[0040] (2) Concentration and separation: the effluent obtained in step (1) is left to settle for 25 minutes in an inclined water tank; the water tank is a cuboid water tank, and the aspect ratio in the tank is 2.5:1, and the angle between the water tank and the horizontal plane is 10°; along the inclined di...

Embodiment 3

[0043] A quick cleaning method for entomopathogenic nematodes, comprising the steps of:

[0044] (1) Cleaning: Put the culture mixture obtained above into the washing bucket of the washing machine (water injection volume is 40L), the volume ratio of the water injection volume in the washing machine to the culture mixture is 4:1; Washing 12min-dehydration 2min-rinsing 5min-dehydration 2min-rinsing 5min-dehydration 2min, the total cleaning time is 28min, and the washing machine discharge liquid is collected; the speed of the single washing is 40rpm, and the speed of the rinsing is 40rpm, The rotation speed of the dehydration is 500rpm; about 97% of the nematodes are in the discharge liquid.

[0045] (2) Concentration and separation: the effluent obtained in step (1) is left to settle for 30 minutes in an inclined water tank; is 45°; along the inclined direction, the lower side of the water tank is provided with a clear liquid outlet, and the distance between the clear liquid ou...

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Abstract

The invention provides a quick cleaning method for entomopathogenic nematodes. The method comprises the steps of: putting the culture mixture obtained by the entomopathogenic nematode solid culture method into the washing bucket of the washing machine, and setting the laundry program of the washing machine to perform single washing-dehydration-rinsing-dehydration or single washing-dehydration-rinsing in sequence ‑dehydration‑rinsing‑dehydration, and collect the discharge liquid from the washing machine; let the obtained discharge liquid stand for sedimentation, discard the supernatant; then stir and wash the sediment with distilled water to obtain the concentrated solution of entomopathogenic nematodes; finally add benzisothiazoline‑3 ‑Ketone, absorbed by sponge, sealed package and stored at low temperature. The method of the invention is simple, the equipment used is easy to obtain, can effectively improve the recovery efficiency of nematodes, shorten recovery time, cause little damage to nematodes, improve the quality and quality of nematodes, and obtain nematodes with high survival rate and strong infecting power.

Description

technical field [0001] The invention relates to a quick cleaning method for entomopathogenic nematodes, belonging to the technical field of biological reproduction and cultivation. Background technique [0002] Entomopathogenic nematodes are nematodes that parasitize insects in nature, including Steinernematidae carpocapsae (Sc-2) and Herorhabditidaebacteriophora (Hb-1); such nematodes infect in the third instar The infective juvenile (IJ) enters the insect body from the insect's stomata, mouth, anus or intersegmental membrane, and then releases the genus Xenorhabdus (symbiosis with Nematode stinkii) or the genus Photorhabdus (symbiosis with Nematode elegans) that it carries. Symbiotic) Symbiotic bacteria, nematodes and symbiotic bacteria multiply and release toxic substances that cause insect death. Entomopathogenic nematodes can effectively control soil-dwelling and borer pests of agricultural, forestry and horticultural plants, such as chive maggots, grubs, peach borer, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B03B7/00B03B9/00
Inventor 胡红涛苏本玉万连步范玲超张新梅李曰鹏
Owner KINGENTA ECOLOGICAL ENG GRP
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