Method for extracting amniotic stem cells

An extraction method and stem cell technology, applied in the direction of cell dissociation method, embryonic cells, animal cells, etc., can solve the problems of taking a long time, not easy to collect cells, affecting the cells to climb out, etc., and achieve the effect of uniform drying degree
CN112280733APending Publication Date: 2021-01-29上海坤爱生物科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海坤爱生物科技股份有限公司
Publication Date
2021-01-29

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Abstract

The invention discloses a method for extracting amniotic stem cells. The method comprises the following steps: A1, mechanically separating amniotic epidermis from a healthy placenta, and performing washing; a2, placing a first culture dish in a second culture dish, wherein a plurality of holes are formed in the bottom surface of the first culture dish; a3, cutting the cleaned amnion into pieces, putting the amnion pieces into a first culture dish, adding a buffer solution, then taking out the first culture dish, and paving cut amnion tissue blocks at the bottom of the first culture dish; and A4, putting the first culture dish with the amnion tissue block into another second culture dish again, adding the culture solution for culture, allowing standing for 15-18 days, adding pancreatin fordigestion, then adding the culture solution to stop digestion, taking out the first culture dish, and then extracting stem cells from the culture solution mixed with the cells. The method can effectively solve the problems of high cost and low efficiency in the prior art and the operation complexity caused by a manual inoculation mode, can quickly achieve extraction operation, reduces the cost andimproves the working efficiency.
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Description

technical field

[0001] The invention belongs to the technical field of stem cell extraction and culture, and in particular relates to a method for extracting amniotic membrane stem cells. Background technique

[0002] Amniotic stem cells are stem cells isolated from the amniotic membrane of newborn fetuses, including amniotic epithelial stem cells and amniotic mesenchymal stem cells. In addition, a large number of stem cells can also be isolated from amniotic fluid. Since amniotic fluid is not easy to collect and is easily polluted, the application of amniotic fluid-derived stem cells is also limited.

[0003] Advantages of amniotic stem cells

[0004] (1) Amniotic membrane stem cells (including amniotic membrane epithelial stem cells and amniotic membrane mesenchymal stem cells) have abundant sources, simple isolation process, strong multi-differentiation potential, no ethical barriers, strong proliferation ability, no tumorigenic effect in vivo and in vitro, and low immun...

Claims

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