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Hizikia fusiforme juvenile sporophyte growth regulating method

A technology for young sporophytes and hijiki, which is applied in the field of regulation of the growth of young sporophytes of hijiri, can solve the problems of low seedling success rate and seedling contribution less than 10%, and achieves the improvement of photosynthetic rate and seedling success rate. , the effect of enhancing the ability

Inactive Publication Date: 2019-06-28
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two main ways to produce seedlings of Hijiki: one is to rely on rhizoid regeneration to cultivate rhizoid seedlings, which requires a large number of wild or farmed algae; the other is to use sexual reproduction to collect fertilized The method of ovum or young sporophyte, but the success rate of seedling cultivation of this method is still very low at present, and the contribution in the production seedling is less than 10%

Method used

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  • Hizikia fusiforme juvenile sporophyte growth regulating method
  • Hizikia fusiforme juvenile sporophyte growth regulating method
  • Hizikia fusiforme juvenile sporophyte growth regulating method

Examples

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Embodiment 1

[0040](1) 10 pieces of square cloth strips with uniform size, healthy growth and evenly distributed Sargassum juvenile sporophytes (each piece of cloth strip size is 20mm×20mm, with about 20 young sporophytes attached, each The initial length of strain young sporophyte is 2.5-3.5mm), puts into two 5000mL Erlenmeyer flasks (each Erlenmeyer flask is put into 10 cloth strips) of adding 5L natural seawater, in described natural seawater (contrast seawater) NO 3 - The concentration is 10 μmol L -1 , PO 4 3- The concentration is 0.5μmol L -1 , On this basis, add inorganic nitrogen salts to the two Erlenmeyer flasks, and do not add phosphorus salts. The amount of inorganic nitrogen salt added in the two Erlenmeyer flasks was 0 micromole and 200 micromole per liter of seawater, marked as N-P0 and N+P0 respectively, and the two groups were compared with each other.

[0041] (2) pump air into the Erlenmeyer flask for cultivating hijiki young sporophytes from the outside by an air ...

Embodiment 2

[0046] (1) 10 pieces of square cloth strips with uniform size, healthy growth and evenly distributed Sargassum juvenile sporophytes (each piece of cloth strip size is 20mm×20mm, with about 20 young sporophytes attached, each The initial length of strain young sporophyte is 2.5-3.5mm), puts into two 5000mL Erlenmeyer flasks (each Erlenmeyer flask is put into 10 cloth strips) of adding 5L natural seawater, in described natural seawater (contrast seawater) NO 3 - The concentration is 20μmol L -1 , PO 4 3- The concentration is 1 μmol L -1 , on this basis, add inorganic nitrogen salt and inorganic phosphorus salt to two Erlenmeyer flasks respectively. The amount of inorganic nitrogen salt added to the two Erlenmeyer flasks is 0 and 200 micromoles per liter of seawater, respectively, and the amount of inorganic phosphorus salt added to the two Erlenmeyer flasks is 15 micromoles per liter of seawater, respectively marked as N-P15 and N+P15 , the two groups are compared with eac...

Embodiment 3

[0052] (1) 10 pieces of square cloth strips with uniform size, healthy growth, and evenly distributed Sargassum juvenile sporophytes (each cloth strip size is 20mm×20mm, with about 20 young sporophytes attached, each The initial length of strain young sporophyte is 2.5-3.5mm), puts into two 5000mL Erlenmeyer flasks (each Erlenmeyer flask is put into 10 cloth strips) of adding 5L natural seawater, in described natural seawater (contrast seawater) NO 3 - The concentration is 16μmol·L -1 , PO 4 3- The concentration is 0.8μmol·L -1 , on this basis, add inorganic nitrogen salt and inorganic phosphorus salt to two Erlenmeyer flasks. The amount of inorganic nitrogen salt added to the two Erlenmeyer flasks is 0 and 200 micromoles per liter of seawater, respectively, and the amount of inorganic phosphorus salt added to the two Erlenmeyer flasks is 60 micromoles per liter of seawater, respectively marked as N-P60 and N+P60 , the two groups are compared with each other.

[0053] (...

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Abstract

The invention discloses a hizikia fusiforme juvenile sporophyte growth regulating method which includes the steps: co-culturing hizikia fusiforme male and female receptacles and collecting zygotes inMay to July; taking seawater as a culture environment for culturing the zygotes; adding inorganic nitrogen salt and inorganic phosphor salt into a culture container; continuously pumping air to stir the seawater, regulating the growth length of hizikia fusiforme juvenile sporophyte frond according to the content of the inorganic nitrogen salt and the inorganic phosphor salt in the enriched seawater, enhancing photosynthesis of hizikia fusiforme juvenile sporophyte and enabling the hizikia fusiforme juvenile sporophyte frond to be stronger. According to the method, the inorganic nitrogen salt and the inorganic phosphor salt are added into the culture seawater, a proper ecological environment condition is provided for sexual propagation of hizikia fusiforme, hizikia fusiforme fertilized ovaand survival, growth and development of the juvenile sporophyte are manually regulated, pigment synthesis of the frond, nutrient accumulation and growth and development of the hizikia fusiforme juvenile sporophyte are promoted, and seedling culture success rate is increased.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for regulating the growth of hijiki young sporophytes. Background technique [0002] Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta)] belongs to Phaeophyta, Round subclass, Fucusaceae, Sargasceae, and Sargassum genus. In Japan, Hizikia fusiformis is usually classified as Sargasaceae. New genus Hizikia. Hijiki is a perennial seaweed in the warm temperate zone. It is a unique species in the western North Pacific Ocean. It is distributed on the rocky reefs in the low tide zone along the coasts of Korea, Japan and my country. It is widely distributed in the north and south of my country, especially in Zhejiang, Fujian and Guangdong. The coastal resources are relatively rich. . [0003] Hijiki is dioecious, spends the winter on the remaining rhizomes, and regenerates plants from the rhizomes in the following spring. During the reproductive sea...

Claims

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Application Information

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IPC IPC(8): A01G33/00
Inventor 邹定辉温嘉怡蔡逸洵龚静雨
Owner SOUTH CHINA UNIV OF TECH
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