90 results about "Pigment synthesis" patented technology

The invention provides a biological coloring and flavoring method for tobacco products. In the tobacco processing procedure, tobacco or tobacco extract liquid is used as a main raw material, the main raw material undergoes microbial fermentation to biosynthesize natural pigment, and the natural pigment is added back to the tobacco products to obtain colored and flavored tobacco products. The method includes the steps of tobacco extract liquid culture medium preparation, fermentation seed liquid preparation, natural pigment synthesis, pigment extraction and back adding of the pigment to the tobacco products. The method is suitable for coloring and flavoring of the tobacco products in the technology of flavoring of tobacco sheets, cut stems, flue-cured tobacco laminas and cigarettes. According to the method, tobacco waste is converted into the natural pigment in a biological mode, the natural pigment is used as a coloring adjunct to be used in the tobacco products and replaces chemical pigment, color and flavor are enhanced at the same time, and the two purposes are achieved; on-line digestion and utilization of the tobacco waste and liquid waste can be achieved, pollutant discharge is reduced, and good social benefits, environment benefits and economic benefits are generated.

The invention discloses a Yunnan red pear PyMYB gene through pigment synthesis regulation and trichome development regulatory protein as well as a prokaryotic expression vector thereof and an application of the prokaryotic expression vector. The PyMYB gene is cloned from red fruit peel of Yunnan red pear NO.1 by using a RT-PCR (reverse transcription-polymerase chain reaction) technology, and then the gene is expressed in escherichia coli Rosetta (DE3) by using the prokaryotic expression vector and is purified by a GST (Glutathione S-transferase) gel column purification method to obtain a Yunnan red pear PyMYB purified protein, wherein the Yunnan red pear PyMYB purified protein is used for the preparation of a PyMYB specific antibody and the application thereof in a PyMYB protein expression detection and immunoprecipitation, a chromatin co-immunoprecipitation and a fusion protein sedimentation experiment. The antibody prepared by the invention is strong in specificity, can accurately detect subcellular localization of the PyMYB protein, and is used for verifying protein interaction in the co-immunoprecipitation experiment, efficiently separating the protein and DNA (deoxyribonucleic acid) segment combined by the PyMYB protein and detecting the expression of the antibody in a transgenic plant.

The invention discloses a colored cotton ovule in vitro culture method, which comprises the steps of: collecting 0-2 days old young bolls of green cotton or brown cotton of the day, pulling off the corolla, stripping calyxes, and retaining flower stalks; washing the young bolls with distilled water twice, conducting disinfection with 75% alcohol for 5-15min under a sterile condition, then performing soaking with 0.1% mercury chloride for 5-15min, and conducting washing with sterile water 5-8 times, and stripping ovules; inoculating the ovules under a sterile condition into a BT medium containing hormone ZR, GA3 and IAA, inoculating 15-25 ovules of one ovary into each bottle, and carrying out dark culture for 30-40d at 30-32DEG C. Preferably, during inoculation of green cotton ovules, the hormone ratio wais GA31.0micromol / L+IAA5.0micromol / L+ZR2.5micromol / L, and during inoculation of brown cotton ovules, the hormone ratio is GA31.0micromol / L+IAA1.0micromol / L+ZR1.0micromol / L. The method provided by the invention can reduce the contamination rate, improve the survival rate, and promote cotton fiber growth and pigment synthesis.

The invention discloses derivatives of naftifine hydrochloride as well as a preparation method and application thereof. The derivatives of naftifine hydrochloride are as shown in the formula I. By inhibiting expression and / or function of key catalyzing enzyme CrtN in a golden yellow pigment synthesis pathway and powerfully inhibiting synthesis of golden yellow pigment, virulence of bacteria can be lowered. The key catalyzing enzyme CrtN in the golden yellow pigment synthesis pathway can be taken as a drug acting target; and compounds capable of inhibiting expression and / or function of key catalyzing enzyme CrtN can be used for preparing anti-bacterial drug. The naftifine hydrochloride and the derivatives thereof can be used as inhibitors for catalyzing enzyme CrtN to powerfully inhibit synthesis of the golden yellow pigment, so that the virulence of staphylococcus aureus can be lowered, and therefore, the derivatives can be used for preparing the anti-bacterial drug, especially drug for resisting staphylococcus aureus infection.

The invention belongs to the field of pigment synthesis and provides a synthesis method of azoic coupling component AS-D series pigment for an aqueous application system and a product obtained by the synthesis method. The synthesis method comprises the steps of coupling azoic coupling component AS-D as a coupling component with substituted aromatic amine diazonium salt to obtain the azoic coupling component AS-D series pigment with a structure satisfying a general formula (I), wherein sulfonated succinic acid surfactant activated agent with the structure satisfying a formula (II) is used, andthe formulas (I) and (II) are as shown in the specification, in the formulas, R1 is selected from CH3, NO2, OCH3 or Cl, R2 is selected from NO2, H, Cl, CH3 or SO2NHCH3, R3 is selected from H, Cl, NO2or CONH2, and R is selected from C4H9, C5H11,C6H13, C8H17, C10H21, C12H25, C13H27 or C18H37. The invention also provides the product obtained by the synthesis method. By using the synthesis method, the problems that in the existing pigment synthesis, chemical oxygen demand (COD) in waste water is over high, environment is polluted, and the like are solved. The pigment product prepared by the synthesis method is easy to disperse in aqueous solution and has bright color and high tinting power.

The invention discloses a method for improving the color of chromium oxide green, and aims to solve the problem that the tone of the traditional chromium oxide green prepared by thermally decomposing chromic anhydride is dark. The method comprises the following steps of: A, adding an additive in an amount which is 2 to 5 percent of the mass of the chromic anhydride, uniformly mixing the additive and the chromic anhydride, putting into a rotary kiln, and performing thermal decomposition reaction, wherein the additive is one of ammonium chloride, potassium fluoride and sodium fluoride; B, controlling the temperature of a high-temperature zone of the rotary kiln to 1,050-1,100 DEG C, feeding into the rotary kiln, and roasting for 3 to 4 hours; and C, grinding the chromium oxide green obtained by roasting into 600-mesh powder with the passing rate of over 90 percent, packaging and delivering after the product is qualified through detection. In order to improve the color brightness and coloring power of the chromic anhydride chromium green, the additive is added in the process of preparing the chromium oxide green by thermally decomposing the chromic anhydride to promote pigment synthesis reaction, and reducing atmosphere when the chromic anhydride is subjected to thermal decomposition is enhanced, so the chromium oxide green obtained by thermal decomposition has bright color, and the brightness and coloring power of the chromium oxide green are increased.

There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

A modified organic pigment can be used to make a coating composition, such as a paint, a varnish, or a printing ink. The modified organic pigment is obtained by reacting the pigments during the pigment synthesis with at least one interface-active compound, where the interface-active compound is a compound within a three-dimensional Hansen space determined by three coordinates, Hansen solubility parameters (HSPs), D=18.21, P=8.44, H=13.16 with a radius of 6.7 units about a center of the three-dimensional Hansen space, determined with the aid of the HSPiP software, version 5.0.0.4.

The invention discloses a whole-spike bagging safe production method for tomato fruits, a special fruit bag which has warming and heat preservation functions consists of an outer bag made of a colorless transparent plastic film and an inner bag made of black paper, the bagging is carried out in the initial stage of tomato inflorescence fruit setting, and after the fruits are ripe, the whole spike is harvested and marketed. The method is used for greenhouse tomato production in winter and can promote fruit expanding obviously, improve pigment synthesis capability, ensure the fruit coloring to be uniform and the fruits to expand, shorten the fruit ripening period and obviously reduce pesticide residue pollution to the fruits, and the method can be directly popularized and applied to the greenhouse tomato production in winter.

An industrial production method for a red with small precipitation on a machine surface and a method for detecting precipitation on a machine surface and belong to the technical field of pigment synthesis and detection. Amino chlorotoluene sulfonic acid sodium salt is subjected to acidification to obtain a turbid liquid; a sodium nitrite water solution is added to achieve diazotization so as to obtain a diazotized solution; the diazotized solution and 3-Hydroxy-2-naphthoic acid are subjected to coupled reaction with existence of a dispersant, and the obtained product is laked to obtain a red. Before laking, the dispersant is added to coat the pigment so as to obstruct growth of the pigment crystal form, the dispersity is improved, the applicability of the machine surface precipitation during application of the blue-ray red to plastics is remarkably improved, and the economic benefits are improved; the reaction conditions are mild, the requirements on equipment are low, and the reaction time is short; the dispersant is relatively common, the application difficulty of a modifier is relatively low, the performance of the obtained blue-ray red is good, the heat resistance can reach 220-230 DEG C, and the transference resistance is of 4 grade.

The invention discloses a preparation method of a blue inkjet ceramic pigment. The preparation method of the blue inkjet ceramic pigment comprises the following steps of: (1), preparing seed crystal, namely, uniformly mixing cobaltous hydroxide, aluminum oxide and aluminum hydroxide in a molar ratio of (0.4-0.5):(0.4-0.6):(0.01-0.06) to obtain a mixture, mixing the mixture with agate ball stone and water in a weight ratio of 100:(450-550):(54-66) and carrying out ball milling until the grain size of the ball-milled mixture reaches D90 not more than 5 microns, drying and screening the ball-milled mixture, and adding boride which is 3%-5% of total weight to the ball-milled mixture, uniformly mixing, roasting, carrying out ball milling and drying to obtain the seed crystal; (2), preparing the pigment, namely, uniformly mixing the cobaltous hydroxide, aluminum oxide and aluminium hydroxide in the molar ratio of (0.4-0.5):(0.4-0.6):(0.01-0.06) to obtain the mixture, adding the seed crystal which accounts for 1%-5% of the total weight and boride which accounts for 3%-5% of the total weight, uniformly mixing, roasting, carrying out ball milling and drying to obtain the finished product. The blue inkjet ceramic pigment has improved color development strength and improved product stability; and meanwhile, the preparation method disclosed by the invention is simple in process and low in pigment synthesis difficulty.

The present invention is directed to compositions containing a pigment synthesis inhibitor herbicide selected from the group consisting of a pyridazinone, isoxazolidinone, triketone, and isoxazole, and pyroxasulfone in a ratio of pigment synthesis inhibitor herbicide to pyroxasulfone of from about 1:0.3 to about 1:150. The present invention is further directed to methods of increasing the activity of a pigment synthesis inhibitor herbicide with the compositions of the present invention.

The invention belongs to the field of pigment synthesis, and particularly relates to a production system and a process method for continuously synthesizing a bisazo pigment. The method comprises the following steps: carrying out diazotization reaction on a hydrochloric acid solution of a diazo component and a sodium nitrite solution in a membrane dispersion microreactor to prepare a diazonium saltsolution A; preparing a coupling component solution B from a coupling component and sodium hydroxide; preparing acetic acid and sodium acetate into a buffer solution C with the pH value of 4.3; and simultaneously introducing the solution A, the solution B and the solution C into a double-sided microporous dispersion microreactor according to a certain flow ratio, carrying out a coupling reactionto generate a bisazo pigment crude product, and further treating the crude product to obtain the azo pigment product. The continuous diazotization reaction is realized by adopting the membrane dispersion microreactor, the continuous coupling reaction is realized by adopting the double-sided microporous dispersion reactor, and the continuous synthesis of the bisazo pigment is realized through the two reactions. The invention has the advantages of simple process, short reaction period, easiness in temperature control and easiness in pH adjustment.

The invention discloses an industrial production and detection method of excellent migration-resistant heat-resistant pigment red PR-48: 2 and belongs to the technical field of pigment synthesis and detection. The method comprises carrying out an acidification reaction process on sodium 2-amino-4-chloro-5-methylbenzenesulfonate to obtain a suspending liquid, adding a sodium nitrite aqueous solution into the suspending liquid, carrying out diazotization to obtain a diazotized solution, carrying out coupling on the diazotized solution and 3-hydroxy-2-naphthoic acid in the presence of a dispersant to obtain a product, carrying out lake treatment on the product to obtain pigment red, drying and crushing the pigment red to obtain toner particles, mixing the toner particles, PU resin and a solvent DMF according to a certain ratio, carrying out high-rate beating homogenization, coating a release paper with the slurry through a scraper, carrying out high temperature baking, tearing the coating to obtain a coloured release paper and comparing a migration degree through a color measurement instrument. The method has mild reaction conditions, low equipment requirements and short reaction time, is convenient for operation and utilizes a common dispersant. The product paratonere has good performances and migration resistance of 5 level and resists a temperature of 210-220 DEG C.

The invention discloses a Yunnan red pear pigment synthesis and trichome development regulatory protein PyTTG1 gene delta PyTTG1 and a prokaryotic expression vector thereof. The Yunnan red pear delta PyTTG1 purified protein is obtained by cloning the specific segment delta PyTTG1 of the PyTTG1 gene from the Yunnan red pear, constructing the prokaryotic expression vector of the gene and expressing the gene in the Escherichia coli. The Yunnan red pear delta PyTTG1 purified protein is applied to preparation of a PyTTG1 specific antibody, detection on PyTTG1 protein expression, immunoprecipitation, and chromatin immunoprecipitation. The antibody has high specificity and can accurately detect the subcellular localization of the PyTTG1 protein, efficiently purify the PtTTG1 protein, efficiently separate the protein bound with the PyTTG1 protein from the DNA segment, and detect the expression condition of the the PtTTG1 protein in transgenic plants.

The invention belongs to the technical field of pigment synthesis and discloses a low-hexavalent-chromium precipitated copper and chromium black pigment as well as a preparation method and applicationthereof. The method comprises the following steps: (1) preparing a nitrate solution, preparing raw materials into the nitrate solution containing Cu<2+>, Cr<3+> and Fe<3+>; (2) preparing a combustionsolution, mixing the nitrate solution and auxiliary fuel to obtain the combustion solution; (3) carrying out low-temperature self-propagating combustion: after evaporating and concentrating the combustion solution, heating the solution in a heating device and initiating the self-propagating combustion; collecting a combustion product; (4) mixing and carrying out ball milling: carrying out the ball mixing on the combustion product and inorganic salt and mixing to obtain a mixture; (5) carrying out high-temperature calcining: putting the mixture into the heating device and carrying out the high-temperature calcining; (6) carrying out post-treatment: carrying out water washing on a product obtained by the high-temperature calcining and drying to obtain the copper and chromium black pigment.The copper and chromium black pigment prepared by the preparation method has the advantages of high purity, complete crystalline grains, good dispersity and low hexavalent chromium content; the pigment has a small average grain diameter which can reach 0.5mu m.

The invention belongs to the technical field of biology engineering and particularly relates to an NtWRKY69 gene affecting tobacco pigment content and an application of the NtWRKY69 gene. The gene comprises a 918bp base with the base sequence shown in SEQ ID NO.1, wherein 80th-600th nucleotides are specific core fragments. Through cloning of the NtWRKY69 gene of tobacco and analysis of corresponding protein, the gene is found to play important roles in regulating tobacco pigment synthesis ways and be highly correlated with the content of pigments in plant leaves. Further, by the aid of a virus mediated gene silencing technology, after the NtWRKY69 gene is silent, the content of the pigments in new transgenic plants is obviously reduced. By means of the characteristic, a new strategy and path can be provided for gene engineering breeding of the tobacco or breeding of other new plant varieties.

The invention discloses a method for preparing a rosin derivative for the pigment industry and relates to the technical field of deep processing of rosin. The method comprises the following steps of: adding alkylphenol disulfide which accounts for 0.1-1.0% of the total mass of rosin into the rosin and carrying out disproportionation reaction, wherein the reaction temperature is 260-280 DEG C and the reaction time is 2-6 hours; cooling down after the reaction is accomplished; adding tannic acid which accounts for 0.3-1.0% of the total mass of the rosin at 160-200 DEG C; stirring and reacting for 1-3 hours; and taking out the product which is the rosin derivative. Compared with the prior art, the rosin derivative can improve the tinting strength of the pigment, is high in transparency and is nature and environment-friendly. The rosin derivative is mainly applied to industries such as pigment synthesis and is particularly applicable to preparation of benzidine yellow pigment with high additive amount.

The invention provides an N-benzyl benzamide compound as well as a preparation method and application thereof. The N-benzyl benzamide compound is an N-benzyl benzamide compound with a general formulaI or II or a salt thereof. The invention discloses a weeding composition or a mixed preparation taking the N-benzyl benzamide compound or a salt thereof as a component. The dosage of the N-benzyl benzamide compound or a salt thereof is 10 grams to 5 kilograms for each hectare, preferably, the N-benzyl benzamide compound or a salt thereof is applied to soil or weeds by adopting a pre-emergence soiltreatment method or a post-emergence stem and leaf spraying method, and the N-benzyl benzamide compound or a salt thereof can be used as a pigment synthesis inhibitor for preventing and removing weeds in field crop growth places and non-farming crop places.

The invention discloses a process for synthesizing an isoindolinone pigment under mild conditions. The process comprises the following steps of: (a) cyclizing: enabling 3,4,5,6-tetra-substituted ortho-cyano methyl benzoate and a fatty alcohol alkali metal salt to react at room temperature in a solvent to get an alkali metal salt of 3,3-dimethoxy-4,5,6,7-tetra-substituted isoindoline-1-ketone; (b) condensing: performing condensation reaction on the product obtained in the step (a) and ortho, meta or para-phenylene diamine with or without a substituent under reflux conditions in the solvent; and (c) hydrolyzing: adding water into the product obtained in the step (b), hydrolyzing and then collecting the isoindolinone pigment from the reaction product. The process disclosed by the invention has the advantages that an alcoholic solvent is taken as the reaction solvent to get the isoindolinone pigment with high reaction yield and excellent quality, the use of the toxic solvent with high boiling point for reaction at a high temperature can be avoided, the operation is simple, the reaction conditions are mild, and the process is suitable for industrial production.

The invention belongs to the field of pigment synthesis, and particularly relates to a production system and a process method for continuously preparing high-transparency and high-strength monoazo pigment in a micro-reactor. The method comprises the following steps: carrying out diazotization reaction on a hydrochloric acid solution of a diazo component and a sodium nitrite solution in a membranedispersion microreactor to generate a diazonium salt solution, adding a certain amount of acetic acid into the diazonium salt solution, and uniformly mixing to obtain a solution A; preparing a solution B from the coupling component, sodium hydroxide and vulcanized castor oil, carrying out coupling reaction on the solution B and the solution A in a microporous dispersion microreactor to generate apigment crude product, and further treating the pigment crude product to obtain a high-transparency and high-strength azo pigment product. According to the invention, continuous preparation of the monoazo pigment is realized by adopting the membrane dispersion microreactor and the microporous dispersion microreactor, the process is simple, the reaction period is short, and the reaction process iseasy to monitor and control; compared with other types of microreactors, the reactor provided by the invention has the advantages that the reaction yield is improved, the product purity is improved, the particle size of a pigment product generated through the reaction of the microreactor is smaller than that of a commercial standard substance, the particle size distribution is narrower, and the transparency and strength of the product are greatly improved.

The invention relates to the field of synthesis of organic pigments and discloses a preparation method for quinacridone and derivatives of the quinacridone. The preparation method comprises the steps that dimethyl succinate generates sodium dimethyl succinylsuccinate (DMSS) through cyclic condensation under an effect of sodium methoxide, and then being acidized into DMSS; condensation reaction occurs between the DMSS and aniline or substituted aniline, an intermediate product generated during condensation is directly oxidized and dehydrogenated by sodium 3-nitrobenzene sulfonate to obtain an oxidative product; and finally coarse products of the quinacridone or the derivatives of the quinacridone are prepared from the oxidative product in a ring-closing mode under an effect of polyphosphoric acid, and finally finished products of the quinacridone or the derivatives of the quinacridone are obtained through pigmentation treatment.

The invention belongs to the technical field of tobacco gene engineering, and particularly relates to an NtOEP1 gene influencing tobacco pigment content and application thereof. The gene comprises 999bp bases, 153-522 nucleotides are specific core fragments, and the base sequence of the nucleotides is shown as SEQ ID NO.1; 332 amino acids are encoded, and the amino acid sequence is shown as SEQ ID NO. 2. According to the application, by cloning and analyzing the tobacco NtOEP1 gene, the gene has important application in regulation and control of a tobacco pigment synthesis route and is highlyrelated to the content of pigment substances in plant leaves. Furthermore, through a virus-mediated gene silencing technology, the inventor finds that after the NtOEP1 gene is silenced, the content of pigment substances in a new transgenic plant is obviously reduced. By means of the characteristic, a new strategy and path can be provided for tobacco genetic engineering breeding or cultivation ofother plant new varieties.

The present invention belongs to a brown porous inorganic solid pigment synthesis method, which comprises: uniformly mixing an iron salt, a nickel salt, and water glass, adjusting the pH value to 8.5-10.5, stirring vigorously, transferring into to a stainless steel reaction kettle, reacting for 20-130 h, washing, filtering, and drying to obtain brown soapstone; and adding the obtained iron-nickel soapstone to a prepared pillared solution containing alumina sol or trideca polyhydroxy aluminum, aging for 0.5-2.5 h at a temperature of 50-80 DEG C, carrying out filtering, washing and drying on the obtained mixture, and heating for 1.5-5 h at a temperature of 150-310 DEG C to obtain the brown porous inorganic solid compound pigment. The brown porous inorganic solid pigment of the present invention does not contain toxic substances, has characteristics of stability, light sunning resistance, washing resistance, and large specific surface area, can be used for decoration products, cosmetics, paper packing and other fields, and can further be used as the solid sorbent, the decorative pigment, the ink additive, the drying agent, and the like.

The invention contains two aspects, namely, the development of a specific fruit bag for cluster trimming and bagging of tomato fruits and the application technology thereof. The warming fruit bag takes a colorless and transparent plastic film as an outer bag and black paper as an inner bag; and the plastic film of the outer bag has millipores formed by mechanical stamping. After the fruit setting of tomatoes, the whole fruit of a tomato is totally bagged in the fruit bag, thus playing the role of regulating temperature and moisture, promoting the growth and early maturity of tomato fruits, and enhancing pigment synthesis. Through bagging treatment on overwinter tomatoes, ambient temperature for fruit development is increased by more than 3 DEG C, the growth speed is increased by 22.7% compared with contrasts, the mature period is 5-8d early than being expected, the weight of single tomato is increased by more than 15.3%, the lycopene of fruits is increased by 49.72%, and the pesticide residues are also significantly reduced compared with the contrasts.

The invention discloses a hizikia fusiforme juvenile sporophyte growth regulating method which includes the steps: co-culturing hizikia fusiforme male and female receptacles and collecting zygotes inMay to July; taking seawater as a culture environment for culturing the zygotes; adding inorganic nitrogen salt and inorganic phosphor salt into a culture container; continuously pumping air to stir the seawater, regulating the growth length of hizikia fusiforme juvenile sporophyte frond according to the content of the inorganic nitrogen salt and the inorganic phosphor salt in the enriched seawater, enhancing photosynthesis of hizikia fusiforme juvenile sporophyte and enabling the hizikia fusiforme juvenile sporophyte frond to be stronger. According to the method, the inorganic nitrogen salt and the inorganic phosphor salt are added into the culture seawater, a proper ecological environment condition is provided for sexual propagation of hizikia fusiforme, hizikia fusiforme fertilized ovaand survival, growth and development of the juvenile sporophyte are manually regulated, pigment synthesis of the frond, nutrient accumulation and growth and development of the hizikia fusiforme juvenile sporophyte are promoted, and seedling culture success rate is increased.

The invention relates to a novel method for treating chrome-containing wastewater and recycling chrome of chrome pigments. The novel method comprises two parts, namely treating the chrome-containing wastewater and recycling the chrome of the chrome pigments. The treatment to the chrome-containing wastewater of the chrome pigments refers to carrying out pressure filtration on the chrome pigments, carrying out physical impurity removal on the chrome-containing wastewater generated in a washing process, adjusting a pH value and then carrying out exchange adsorption in an ion exchange system to ensure that effluent reaches the emission standards of China; after adsorption saturation by ion exchange resin and fiber, a regenerant is used for carrying out regeneration and transformation to obtain a high-concentration sodium dichromate concentrated solution. The recycling of the chrome refers to taking the sodium dichromate concentrated solution as a raw material for pigment production, and adding the solution to a pigment synthesis system to prepare pigment; the prepared pigment is subjected to pressure filtering, washed, dried, ground and packaged to obtain the finished product. According to the method disclosed by the invention, chrome in the wastewater can be resourcefully recycled while ensuring that hexavalent chrome in the chrome-containing wastewater in the chrome pigments is discharged after reaching the standards; the novel method is can either be big or small in scale, simple in process route, low in energy consumption, high in recovery rate of chrome and free of secondary pollution and has favorable economic benefit and social benefit.

There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.